Table 1.
Primer sequences of targeted genes and GAPDH
| Gene | GenBank accession number | Primer sequences (5’–3’) | Product size (bp) |
|
|---|---|---|---|---|
| TNF-α | NM_173966.3 | CCAGAGGGAAGAGCAGTCCC | 114 | |
| TCGGCTACAACGTGGGCTAC | ||||
| IL-1β | NM_174093 | ATTCTCTCCAGCCAACCTTCATT | 100 | |
| TTCTCGTCACTGTAGTAAGCCATCA | ||||
| IL-6 | NM_173923 | ATGACTTCTGCTTTCCCTACCC | 180 | |
| GCTGCTTTCACACTCATCATTC | ||||
| IL-10 | NM_174088.1 | ACAGGCTGAGAACCACGGGC | 175 | |
| GACACCCCTCTCTTGGAGCTCACT | ||||
| CXCL-1 | NM_175700.2 | CATCCAGAGCGTGAAGGTGA | 100 | |
| GGTGGGGTTGAGACACACTT | ||||
| CXCL-6 | NM_174300.2 | TGAGAGAGCTGCGTTGTGTG | 119 | |
| GGTGGCTATCACTTCCACCT | ||||
| COX-2 | AF031698 | ATCTACCCGCCTCATGTTCCT | 187 | |
| GGATTAGCCTGCTTGTCTGGA | ||||
| iNOS | XM_024979646.1 | CAGGATGACCCCAAACGTCA | 190 | |
| CCTTCTGGTGAAGCGTGTCT | ||||
| p65 | NM_001080242.2 | CTCACCCCATCTTTGACAACC | 152 | |
| TCCCGTGAAATACACCTCGAT | ||||
| IκBα | NM_001045868.1 | TGTGATCCTGAGCTGCGAGAC | 190 | |
| TGCCCAGGTAGCCATGAATAG | ||||
| Akt | NM_173986.2 | AAAAGGAAGTGGTGTACAGG | 80 | |
| GAAGTCGGTGATCTTGATGT | ||||
| PI3K | NM_174575.1 | ACACAGCTGACGGGACCTTT | 127 | |
| CCATATTTCCCATCTCGGTGA | ||||
| GAPDH | NM_001034034.2 | CCCAGAATATCATCCCTGCT | 185 | |
| CTGCTTCACCACCTTCTTGA | ||||