Fig. 1.

PAQR5 is commonly expressed at low levels in ccRCC cells and tissues. A Quantitative real-time PCR (qRT‒PCR) analysis of the mRNA expression levels of PAQR5 in human renal carcinoma cell lines and the normal renal tubular epithelial cell line HK-2. 18S was applied as the endogenous control. Data are presented as the mean ± SEM, n = 3. B Western blot analysis of PAQR5 expression levels in human renal carcinoma cell lines and the normal renal tubular epithelial cell line HK-2. C PAQR5 protein expression levels were analyzed in six cases of fresh human ccRCC tissues and corresponding normal tissues by western blotting assay. β-actin was used as an internal loading control. D Quantification of the western blotting results. E Representative images of PAQR5 protein expression in ccRCC tissues and paired normal kidney tissues in ccRCC tissue chip detected by IHC. The scales bars are 500 μm and 100 μm, respectively. Scatter plots and heatmap showing the IHC scores of PAQR5 in 30 pairs of ccRCC tissues and normal renal tissues. *P < 0.05, **P < 0.01, ***P < 0.001