Fig. 3.

PAQR5 suppresses cell proliferation of ccRCC in vitro. A The efficiency of PAQR5 knockdown in the OS-RC-2 cell line and overexpression in the 786-O cell line was verified by western blotting analysis. The OS-RC-2 cell line was transfected with si-PAQR5-1 or si-PAQR5-2 or si-NC, and the 786-O cell line was transfected with the PAQR5 overexpression vector or negative control vector. β-actin was used as a loading control. B Cell viability was detected by an MTT assay in PAQR5 overexpressing or PAQR5-downregulated ccRCC cells. C An EdU incorporation assay for staining proliferating cells (red indicates EdU-incorporated cells; blue indicates nuclear staining with Hoechst 33342) was conducted in OS-RC-2 and 786-O cells with treatment as indicated. Quantification analysis is shown on the right. Scale bars, 100 μm. D The cell cycle distribution of OS-RC-2 and 786-O cells treated as indicated was analyzed by flow cytometry. The quantitative analysis of the results is shown on the corresponding right. *P < 0.05, **P < 0.01, ***P < 0.001