Fig. 4.
PAQR5 inhibits the migration and invasiveness of ccRCC cells in vitro. A Representative images of wound healing assays in PAQR5 knockdown (KD) OS-RC-2 sublines and PAQR5 overexpressing (OE) 786-O sublines. Quantitative data is shown on the right. B Representative images of transwell migration and invasion in PAQR5-KD OS-RC-2 sublines and PAQR5-OE 786-O sublines. Quantitative data is shown on the corresponding below. Scale bars, 100 μm. C Western blot analysis of p21, Cyclin E1, Cyclin D1, CDK2, E-cadherin, N-cadherin, MMP2, and MMP9 in PAQR5-KD OS-RC-2 sublines and PAQR5-OE 786-O sublines. β-actin was used as an internal loading control. **P < 0.01; ***P < 0.001. KD: knockdown; OE: overexpression
