Fig. 5.

PAQR5 represses the IL6-JAK-STAT3 signaling pathway in ccRCC cells. A GSEA results showed that “HALLMARK_IL6_JAK_STAT3_SIGNALING” was enriched in the PAQR5-low expression group. B JAK2, P-JAK2, STAT3, and P-STAT3 were evaluated by western blotting assay in PAQR5-KD OS-RC-2 and PAQR5-OE 786-O sublines. β-actin was used as a loading control. C The phosphorylation level of STAT3 (Tyr705) in nuclear fractions and cytoplasmic fractions was analyzed by western blotting after treatment as indicated. Histone H3 and β-actin were used as the loading controls for the nucleus and cytoplasm, respectively. D Immunofluorescence indicated the nuclear translocation of phosphorylated STAT3 (P-STAT3) induced by PAQR5 in the PAQR5-KD OS-RC-2 and PAQR5-OE 786-O sublines. Scale bars, 50 μm