Fig. 6.

PAQR5 inhibits the growth, migration, and invasion of ccRCC cells by curbing the JAK-STAT3 pathway. A The OS-RC-2 cell line was transfected with si-PAQR5 or si-STAT3 alone or with both si-PAQR5 and si-STAT3, while the 786-O cell line was transfected with PAQR5 overexpression plasmids or STAT3 overexpression plasmids alone or with both overexpression plasmids simultaneously. The efficiency of PAQR5 and STAT3 knockdown in the OS-RC-2 cell line and overexpression in the 786-O cell line was verified by detecting the expression of PAQR5 and STAT3 proteins using western blotting analysis. The expression of P-STAT3 (Tyr705), Cyclin E1, Cyclin D1, CDK2, MMP2, and MMP9 were also examined after treatment as indicated. β-actin was used as a loading control. B An MTT assay was conducted to evaluate the viability of OS-RC-2 and 786-O cells after treatment as indicated. C Colony formation assays were also performed in OS-RC-2 and 786-O cells treated as described above. Quantification analysis is shown on the right. D The migration and invasion abilities were analyzed by a transwell Boyden assay without or with Matrigel in OS-RC-2 and 786-O cells after treatment as indicated. Quantification analysis is shown below. Scale bars, 100 μm. **P < 0.01, ***P < 0.001 versus the scrambled si-NC group or control group, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus the si-STAT3 group or PAQR5 group