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. 1998 Apr;74(4):2076–2079. doi: 10.1016/S0006-3495(98)77914-7

Optical chemical imaging of tobacco mosaic virus in solution at 60-nm resolution.

T H Keller 1, T Rayment 1, D Klenerman 1
PMCID: PMC1299548  PMID: 9545066

Abstract

Scanning near-field optical microscopy can provide images with a resolution less than the wavelength of light, and therefore ought in principle to be of great value in studies of biological structures. In this work we show how for the first time images have been obtained of tobacco mosaic virus particles at 60-nm resolution, combined with chemical imaging using monoclonal antibodies under in vitro conditions.

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Selected References

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  1. Dore I., Weiss E., Altschuh D., Van Regenmortel M. H. Visualization by electron microscopy of the location of tobacco mosaic virus epitopes reacting with monoclonal antibodies in enzyme immunoassay. Virology. 1988 Feb;162(2):279–289. doi: 10.1016/0042-6822(88)90467-9. [DOI] [PubMed] [Google Scholar]
  2. Namba K., Stubbs G. Structure of tobacco mosaic virus at 3.6 A resolution: implications for assembly. Science. 1986 Mar 21;231(4744):1401–1406. doi: 10.1126/science.3952490. [DOI] [PubMed] [Google Scholar]
  3. Putman C. A., van der Werf K. O., de Grooth B. G., van Hulst N. F., Greve J. Viscoelasticity of living cells allows high resolution imaging by tapping mode atomic force microscopy. Biophys J. 1994 Oct;67(4):1749–1753. doi: 10.1016/S0006-3495(94)80649-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Saunal H., Van Regenmortel M. H. Kinetic and functional mapping of viral epitopes using biosensor technology. Virology. 1995 Nov 10;213(2):462–471. doi: 10.1006/viro.1995.0019. [DOI] [PubMed] [Google Scholar]

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