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. 1998 Apr;74(4):2080–2088. doi: 10.1016/S0006-3495(98)77915-9

Micropipet-based pico force transducer: in depth analysis and experimental verification.

D A Simson 1, F Ziemann 1, M Strigl 1, R Merkel 1
PMCID: PMC1299549  PMID: 9545067

Abstract

Measurements of forces in the piconewton range are very important for the study of molecular adhesion and mechanics. Recently, a micropipet-based force transducer for this type of experiment was presented (E. Evans, K. Ritchie, and R. Merkel, 1995, Biophys. J., 68:2580-2587). In the present article we give a detailed mechanical analysis of this transducer, including nonlinear effects. An analytical expression for the transducer stiffness at small elongations is given. Using magnetic tweezers (F. Ziemann, J. Rädler, and E. Sackmann, 1994, Biophys. J., 66:2210-2216), we were able to determine the force displacement relation of this transducer experimentally. Forces from approximately 10 pN to 500 pN were applied. Theoretical predictions and experimental results coincide remarkably well.

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Selected References

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  1. Alon R., Hammer D. A., Springer T. A. Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow. Nature. 1995 Apr 6;374(6522):539–542. doi: 10.1038/374539a0. [DOI] [PubMed] [Google Scholar]
  2. Ashkin A., Schütze K., Dziedzic J. M., Euteneuer U., Schliwa M. Force generation of organelle transport measured in vivo by an infrared laser trap. Nature. 1990 Nov 22;348(6299):346–348. doi: 10.1038/348346a0. [DOI] [PubMed] [Google Scholar]
  3. Dammer U., Hegner M., Anselmetti D., Wagner P., Dreier M., Huber W., Güntherodt H. J. Specific antigen/antibody interactions measured by force microscopy. Biophys J. 1996 May;70(5):2437–2441. doi: 10.1016/S0006-3495(96)79814-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Discher D. E., Mohandas N. Kinematics of red cell aspiration by fluorescence-imaged microdeformation. Biophys J. 1996 Oct;71(4):1680–1694. doi: 10.1016/S0006-3495(96)79424-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans E. A. Analysis of adhesion of large vesicles to surfaces. Biophys J. 1980 Sep;31(3):425–431. doi: 10.1016/S0006-3495(80)85069-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Evans E. A. New membrane concept applied to the analysis of fluid shear- and micropipette-deformed red blood cells. Biophys J. 1973 Sep;13(9):941–954. doi: 10.1016/S0006-3495(73)86036-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Evans E. A. Structure and deformation properties of red blood cells: concepts and quantitative methods. Methods Enzymol. 1989;173:3–35. doi: 10.1016/s0076-6879(89)73003-2. [DOI] [PubMed] [Google Scholar]
  8. Evans E. A., Waugh R., Melnik L. Elastic area compressibility modulus of red cell membrane. Biophys J. 1976 Jun;16(6):585–595. doi: 10.1016/S0006-3495(76)85713-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Evans E. A., Waugh R. Osmotic correction to elastic area compressibility measurements on red cell membrane. Biophys J. 1977 Dec;20(3):307–313. doi: 10.1016/S0006-3495(77)85551-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Evans E., Berk D., Leung A. Detachment of agglutinin-bonded red blood cells. I. Forces to rupture molecular-point attachments. Biophys J. 1991 Apr;59(4):838–848. doi: 10.1016/S0006-3495(91)82296-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Evans E., Ritchie K., Merkel R. Sensitive force technique to probe molecular adhesion and structural linkages at biological interfaces. Biophys J. 1995 Jun;68(6):2580–2587. doi: 10.1016/S0006-3495(95)80441-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Evans E, Rawicz W. Entropy-driven tension and bending elasticity in condensed-fluid membranes. Phys Rev Lett. 1990 Apr 23;64(17):2094–2097. doi: 10.1103/PhysRevLett.64.2094. [DOI] [PubMed] [Google Scholar]
  13. Finer J. T., Simmons R. M., Spudich J. A. Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature. 1994 Mar 10;368(6467):113–119. doi: 10.1038/368113a0. [DOI] [PubMed] [Google Scholar]
  14. Florin E. L., Moy V. T., Gaub H. E. Adhesion forces between individual ligand-receptor pairs. Science. 1994 Apr 15;264(5157):415–417. doi: 10.1126/science.8153628. [DOI] [PubMed] [Google Scholar]
  15. Heinrich V., Waugh R. E. A piconewton force transducer and its application to measurement of the bending stiffness of phospholipid membranes. Ann Biomed Eng. 1996 Sep-Oct;24(5):595–605. doi: 10.1007/BF02684228. [DOI] [PubMed] [Google Scholar]
  16. Hochmuth F. M., Shao J. Y., Dai J., Sheetz M. P. Deformation and flow of membrane into tethers extracted from neuronal growth cones. Biophys J. 1996 Jan;70(1):358–369. doi: 10.1016/S0006-3495(96)79577-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Hochmuth R. M., Mohandas N., Blackshear P. L., Jr Measurement of the elastic modulus for red cell membrane using a fluid mechanical technique. Biophys J. 1973 Aug;13(8):747–762. doi: 10.1016/S0006-3495(73)86021-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kishino A., Yanagida T. Force measurements by micromanipulation of a single actin filament by glass needles. Nature. 1988 Jul 7;334(6177):74–76. doi: 10.1038/334074a0. [DOI] [PubMed] [Google Scholar]
  19. Kuo S. C., Lauffenburger D. A. Relationship between receptor/ligand binding affinity and adhesion strength. Biophys J. 1993 Nov;65(5):2191–2200. doi: 10.1016/S0006-3495(93)81277-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Needham D. Measurement of interbilayer adhesion energies. Methods Enzymol. 1993;220:111–129. doi: 10.1016/0076-6879(93)20078-h. [DOI] [PubMed] [Google Scholar]
  21. Pierres A., Benoliel A. M., Bongrand P. Measuring the lifetime of bonds made between surface-linked molecules. J Biol Chem. 1995 Nov 3;270(44):26586–26592. doi: 10.1074/jbc.270.44.26586. [DOI] [PubMed] [Google Scholar]
  22. Shao J. Y., Hochmuth R. M. Micropipette suction for measuring piconewton forces of adhesion and tether formation from neutrophil membranes. Biophys J. 1996 Nov;71(5):2892–2901. doi: 10.1016/S0006-3495(96)79486-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Simmons R. Molecular motors: single-molecule mechanics. Curr Biol. 1996 Apr 1;6(4):392–394. [PubMed] [Google Scholar]
  24. Svoboda K., Schmidt C. F., Schnapp B. J., Block S. M. Direct observation of kinesin stepping by optical trapping interferometry. Nature. 1993 Oct 21;365(6448):721–727. doi: 10.1038/365721a0. [DOI] [PubMed] [Google Scholar]
  25. Tees D. F., Goldsmith H. L. Kinetics and locus of failure of receptor-ligand-mediated adhesion between latex spheres. I. Protein-carbohydrate bond. Biophys J. 1996 Aug;71(2):1102–1114. doi: 10.1016/S0006-3495(96)79312-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ziemann F., Rädler J., Sackmann E. Local measurements of viscoelastic moduli of entangled actin networks using an oscillating magnetic bead micro-rheometer. Biophys J. 1994 Jun;66(6):2210–2216. doi: 10.1016/S0006-3495(94)81017-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zilker A, Ziegler M, Sackmann E. Spectral analysis of erythrocyte flickering in the 0.3-4- microm-1 regime by microinterferometry combined with fast image processing. Phys Rev A. 1992 Dec 15;46(12):7998–8001. doi: 10.1103/physreva.46.7998. [DOI] [PubMed] [Google Scholar]

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