Abstract
The activity of embedded proteins is known to vary with lipid characteristics. Indeed, it has been shown that some cell-membrane proteins cannot function unless certain non-bilayer-forming lipids (i.e., nonzero spontaneous curvature) are present. In this paper we show that membranes exert a line tension on transmembrane proteins. The line tension, on the order of 1-100 kT/protein, varies with the lipid properties and the protein configuration. Thus, membranes composed of different lipids favor different protein conformations. Model predictions are in excellent agreement with the data of Keller et al. (Biophys. J. 1993, 65:23-27) regarding the conductance of alamethicin channels.
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Selected References
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- Aranda-Espinoza H., Berman A., Dan N., Pincus P., Safran S. Interaction between inclusions embedded in membranes. Biophys J. 1996 Aug;71(2):648–656. doi: 10.1016/S0006-3495(96)79265-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bogdanov M., Sun J., Kaback H. R., Dowhan W. A phospholipid acts as a chaperone in assembly of a membrane transport protein. J Biol Chem. 1996 May 17;271(20):11615–11618. doi: 10.1074/jbc.271.20.11615. [DOI] [PubMed] [Google Scholar]
- Cantor R. S. The lateral pressure profile in membranes: a physical mechanism of general anesthesia. Biochemistry. 1997 Mar 4;36(9):2339–2344. doi: 10.1021/bi9627323. [DOI] [PubMed] [Google Scholar]
- Cullis P. R., de Kruijff B. Lipid polymorphism and the functional roles of lipids in biological membranes. Biochim Biophys Acta. 1979 Dec 20;559(4):399–420. doi: 10.1016/0304-4157(79)90012-1. [DOI] [PubMed] [Google Scholar]
- Evans E, Rawicz W. Entropy-driven tension and bending elasticity in condensed-fluid membranes. Phys Rev Lett. 1990 Apr 23;64(17):2094–2097. doi: 10.1103/PhysRevLett.64.2094. [DOI] [PubMed] [Google Scholar]
- Goulian M., Mesquita O. N., Fygenson D. K., Nielsen C., Andersen O. S., Libchaber A. Gramicidin channel kinetics under tension. Biophys J. 1998 Jan;74(1):328–337. doi: 10.1016/S0006-3495(98)77790-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gruner S. M. Intrinsic curvature hypothesis for biomembrane lipid composition: a role for nonbilayer lipids. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3665–3669. doi: 10.1073/pnas.82.11.3665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keller S. L., Bezrukov S. M., Gruner S. M., Tate M. W., Vodyanoy I., Parsegian V. A. Probability of alamethicin conductance states varies with nonlamellar tendency of bilayer phospholipids. Biophys J. 1993 Jul;65(1):23–27. doi: 10.1016/S0006-3495(93)81040-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lundbaek J. A., Maer A. M., Andersen O. S. Lipid bilayer electrostatic energy, curvature stress, and assembly of gramicidin channels. Biochemistry. 1997 May 13;36(19):5695–5701. doi: 10.1021/bi9619841. [DOI] [PubMed] [Google Scholar]
- Rietveld A. G., Koorengevel M. C., de Kruijff B. Non-bilayer lipids are required for efficient protein transport across the plasma membrane of Escherichia coli. EMBO J. 1995 Nov 15;14(22):5506–5513. doi: 10.1002/j.1460-2075.1995.tb00237.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Kruijff B. Biomembranes. Lipids beyond the bilayer. Nature. 1997 Mar 13;386(6621):129–130. doi: 10.1038/386129a0. [DOI] [PubMed] [Google Scholar]