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. 1999 Mar;76(3):1659–1667. doi: 10.1016/S0006-3495(99)77325-X

Electrically excitable normal rat kidney fibroblasts: A new model system for cell-semiconductor hybrids.

W J Parak 1, J Domke 1, M George 1, A Kardinal 1, M Radmacher 1, H E Gaub 1, A D de Roos 1, A P Theuvenet 1, G Wiegand 1, E Sackmann 1, J C Behrends 1
PMCID: PMC1300142  PMID: 10049346

Abstract

In testing various designs of cell-semiconductor hybrids, the choice of a suitable type of electrically excitable cell is crucial. Here normal rat kidney (NRK) fibroblasts are presented as a cell line, easily maintained in culture, that may substitute for heart or nerve cells in many experiments. Like heart muscle cells, NRK fibroblasts form electrically coupled confluent cell layers, in which propagating action potentials are spontaneously generated. These, however, are not associated with mechanical disturbances. Here we compare heart muscle cells and NRK fibroblasts with respect to action potential waveform, morphology, and substrate adhesion profile, using the whole-cell variant of the patch-clamp technique, atomic force microscopy (AFM), and reflection interference contrast microscopy (RICM), respectively. Our results clearly demonstrate that NRK fibroblasts should provide a highly suitable test system for investigating the signal transfer between electrically excitable cells and extracellular detectors, available at a minimum cost and effort for the experimenters.

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Selected References

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