Abstract
To assay cell cycle progression in synchronized culture of yeast we have applied dielectric spectroscopy to its real-time monitoring. The dielectric monitoring is based on the electromagnetic induction method, regarded as a nonelectrode method, which has resolved the problems encountered in measurements with metal electrodes, namely electrode polarization and bubble formation on electrodes. In the synchronized culture with temperature-sensitive cell division cycle mutants, the permittivity of the culture broth showed cyclic changes at frequencies below 300 kHz. The increase and decrease in the cyclic changes of the relative permittivity correspond to the increase in cell length and bud size and to the septum formation between mother and daughter cells, respectively.
Full Text
The Full Text of this article is available as a PDF (100.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Asami K., Gheorghiu E., Yonezawa T. Dielectric behavior of budding yeast in cell separation. Biochim Biophys Acta. 1998 Jul 23;1381(2):234–240. doi: 10.1016/s0304-4165(98)00033-6. [DOI] [PubMed] [Google Scholar]
- Asami K., Yonezawa T. Dielectric analysis of yeast cell growth. Biochim Biophys Acta. 1995 Aug 17;1245(1):99–105. doi: 10.1016/0304-4165(95)00074-l. [DOI] [PubMed] [Google Scholar]
- Asami K., Yonezawa T. Dielectric behavior of non-spherical cells in culture. Biochim Biophys Acta. 1995 Dec 14;1245(3):317–324. doi: 10.1016/0304-4165(95)00116-6. [DOI] [PubMed] [Google Scholar]
- Haase S. B., Lew D. J. Flow cytometric analysis of DNA content in budding yeast. Methods Enzymol. 1997;283:322–332. doi: 10.1016/s0076-6879(97)83026-1. [DOI] [PubMed] [Google Scholar]