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. 1999 Aug;77(2):888–902. doi: 10.1016/S0006-3495(99)76940-7

31P NMR first spectral moment study of the partial magnetic orientation of phospholipid membranes.

F Picard 1, M J Paquet 1, J Levesque 1, A Bélanger 1, M Auger 1
PMCID: PMC1300380  PMID: 10423434

Abstract

Structural data can be obtained on proteins inserted in magnetically oriented phospholipid membranes such as bicelles, which are most often made of a mixture of long and short chain phosphatidylcholine. Possible shapes for these magnetically oriented membranes have been postulated in the literature, such as discoidal structures with a thickness of one bilayer and with the short acyl chain phosphatidylcholine on the edges. In the present paper, a geometrical study of these oriented structures is done to determine the validity of this model. The method used is based on the determination of the first spectral moment of solid-state (31)P nuclear magnetic resonance spectra. From this first moment, an order parameter is defined that allows a quantitative analysis of partially oriented spectra. The validity of this method is demonstrated in the present study for oriented samples made of DMPC, DMPC:DHPC, DMPC:DHPC:gramicidin A and adriamycin:cardiolipin.

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Selected References

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