Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1999 Dec;77(6):3328–3340. doi: 10.1016/S0006-3495(99)77164-X

Spectroscopic properties of the CP43 core antenna protein of photosystem II.

M L Groot 1, R N Frese 1, F L de Weerd 1, K Bromek 1, A Pettersson 1, E J Peterman 1, I H van Stokkum 1, R van Grondelle 1, J P Dekker 1
PMCID: PMC1300604  PMID: 10585955

Abstract

CP43 is a chlorophyll-protein complex that funnels excitation energy from the main light-harvesting system of photosystem II to the photochemical reaction center. We purified CP43 from spinach photosystem II membranes in the presence of the nonionic detergent n-dodecyl-beta,D-maltoside and recorded its spectroscopic properties at various temperatures between 4 and 293 K by a number of polarized absorption and fluorescence techniques, fluorescence line narrowing, and Stark spectroscopy. The results indicate two "red" states in the Q(y) absorption region of the chlorophylls. The first peaks at 682.5 nm at 4 K, has an extremely narrow bandwidth with a full width at half-maximum of approximately 2.7 nm (58 cm(-1)) at 4 K, and has the oscillator strength of a single chlorophyll. The second peaks at approximately 679 nm, has a much broader bandshape, is caused by several excitonically interacting chlorophylls, and is responsible for all 4 K absorption at wavelengths longer than 685 nm. The Stark spectrum of CP43 resembles the first derivative of the absorption spectrum and has an exceptionally small overall size, which we attribute to opposing orientations of the monomer dipole moments of the excitonically coupled pigments.

Full Text

The Full Text of this article is available as a PDF (159.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alfonso M., Montoya G., Cases R., Rodríguez R., Picorel R. Core antenna complexes, CP43 and CP47, of higher plant photosystem II. Spectral properties, pigment stoichiometry, and amino acid composition. Biochemistry. 1994 Aug 30;33(34):10494–10500. doi: 10.1021/bi00200a034. [DOI] [PubMed] [Google Scholar]
  2. Boekema E. J., Hankamer B., Bald D., Kruip J., Nield J., Boonstra A. F., Barber J., Rögner M. Supramolecular structure of the photosystem II complex from green plants and cyanobacteria. Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):175–179. doi: 10.1073/pnas.92.1.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boekema E. J., van Roon H., Calkoen F., Bassi R., Dekker J. P. Multiple types of association of photosystem II and its light-harvesting antenna in partially solubilized photosystem II membranes. Biochemistry. 1999 Feb 23;38(8):2233–2239. doi: 10.1021/bi9827161. [DOI] [PubMed] [Google Scholar]
  4. Boekema E. J., van Roon H., Dekker J. P. Specific association of photosystem II and light-harvesting complex II in partially solubilized photosystem II membranes. FEBS Lett. 1998 Mar 6;424(1-2):95–99. doi: 10.1016/s0014-5793(98)00147-1. [DOI] [PubMed] [Google Scholar]
  5. Eijckelhoff C., van Roon H., Groot M. L., van Grondelle R., Dekker J. P. Purification and spectroscopic characterization of photosystem II reaction center complexes isolated with or without Triton X-100. Biochemistry. 1996 Oct 1;35(39):12864–12872. doi: 10.1021/bi960991m. [DOI] [PubMed] [Google Scholar]
  6. Groot M. L., Peterman E. J., van Kan P. J., van Stokkum I. H., Dekker J. P., van Grondelle R. Temperature-dependent triplet and fluorescence quantum yields of the photosystem II reaction center described in a thermodynamic model. Biophys J. 1994 Jul;67(1):318–330. doi: 10.1016/S0006-3495(94)80483-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Groot M. L., Peterman E. J., van Stokkum I. H., Dekker J. P., van Grondelle R. Triplet and fluorescing states of the CP47 antenna complex of photosystem II studied as a function of temperature. Biophys J. 1995 Jan;68(1):281–290. doi: 10.1016/S0006-3495(95)80186-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hankamer B., Morris E. P., Barber J. Revealing the structure of the oxygen-evolving core dimer of photosystem II by cryoelectron crystallography. Nat Struct Biol. 1999 Jun;6(6):560–564. doi: 10.1038/9341. [DOI] [PubMed] [Google Scholar]
  9. Hankamer Ben, Barber James, Boekema Egbert J. STRUCTURE AND MEMBRANE ORGANIZATION OF PHOTOSYSTEM II IN GREEN PLANTS. Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48(NaN):641–671. doi: 10.1146/annurev.arplant.48.1.641. [DOI] [PubMed] [Google Scholar]
  10. Hasler L, Ghanotakis D, Fedtke B, Spyridaki A, Miller M, Müller SA, Engel A, Tsiotis G. Structural Analysis of Photosystem II: Comparative Study of Cyanobacterial and Higher Plant Photosystem II Complexes. J Struct Biol. 1997 Aug;119(3):273–283. doi: 10.1006/jsbi.1997.3889. [DOI] [PubMed] [Google Scholar]
  11. Peterman E. J., Wenk S. O., Pullerits T., Pâlsson L. O., van Grondelle R., Dekker J. P., Rögner M., van Amerongen H. Fluorescence and absorption spectroscopy of the weakly fluorescent chlorophyll a in cytochrome b6f of Synechocystis PCC6803. Biophys J. 1998 Jul;75(1):389–398. doi: 10.1016/S0006-3495(98)77523-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peterman E. J., van Amerongen H, van Grondelle R, Dekker J. P. The nature of the excited state of the reaction center of photosystem II of green plants: a high-resolution fluorescence spectroscopy study. Proc Natl Acad Sci U S A. 1998 May 26;95(11):6128–6133. doi: 10.1073/pnas.95.11.6128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rhee K. H., Morris E. P., Barber J., Kühlbrandt W. Three-dimensional structure of the plant photosystem II reaction centre at 8 A resolution. Nature. 1998 Nov 19;396(6708):283–286. doi: 10.1038/24421. [DOI] [PubMed] [Google Scholar]
  14. Schubert W. D., Klukas O., Saenger W., Witt H. T., Fromme P., Krauss N. A common ancestor for oxygenic and anoxygenic photosynthetic systems: a comparison based on the structural model of photosystem I. J Mol Biol. 1998 Jul 10;280(2):297–314. doi: 10.1006/jmbi.1998.1824. [DOI] [PubMed] [Google Scholar]
  15. Somsen O. J., van Grondelle R., van Amerongen H. Spectral broadening of interacting pigments: polarized absorption by photosynthetic proteins. Biophys J. 1996 Oct;71(4):1934–1951. doi: 10.1016/S0006-3495(96)79392-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Visschers R. W., Chang M. C., van Mourik F., Parkes-Loach P. S., Heller B. A., Loach P. A., van Grondelle R. Fluorescence polarization and low-temperature absorption spectroscopy of a subunit form of light-harvesting complex I from purple photosynthetic bacteria. Biochemistry. 1991 Jun 11;30(23):5734–5742. doi: 10.1021/bi00237a015. [DOI] [PubMed] [Google Scholar]
  17. van Amerongen H., Vasmel H., van Grondelle R. Linear dichroism of chlorosomes from chloroflexus aurantiacus in compressed gels and electric fields. Biophys J. 1988 Jul;54(1):65–76. doi: 10.1016/S0006-3495(88)82931-X. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES