Abstract
Red blood cells (RBCs) in the presence of plasma proteins or other macromolecules may form aggregates, normally in rouleaux formations, which are dispersed with increasing blood flow. Experimental observations have suggested that the spontaneous aggregation process involves the formation of linear rouleaux (FLR) followed by formation of branched rouleaux networks. Theoretical models for the spontaneous rouleaux formation were formulated, taking into consideration that FLR may involve both "polymerization," i.e., interaction between two single RBCs (e + e) and the addition of a single RBC to the end of an existing rouleau (e + r), as well as "condensation" between two rouleaux by end-to-end addition (r + r). The present study was undertaken to experimentally examine the theoretical models and their assumptions, by visual monitoring of the spontaneous FLR (from singly dispersed RBC) in plasma, in a narrow gap flow chamber. The results validate the theoretical model, showing that FLR involves both polymerization and condensation, and that the kinetic constants for the above three types of intercellular interactions are the same, i.e., k(ee) = k(er) = k(rr) = k, and for all tested hematocrits (0.625-6%) k < 0.13 +/- 0.03 s(-1).
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- Alonso C., Pries A. R., Gaehtgens P. Time-dependent rheological behavior of blood at low shear in narrow vertical tubes. Am J Physiol. 1993 Aug;265(2 Pt 2):H553–H561. doi: 10.1152/ajpheart.1993.265.2.H553. [DOI] [PubMed] [Google Scholar]
- Barshtein G., Tamir I., Yedgar S. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Eur Biophys J. 1998;27(2):177–181. doi: 10.1007/s002490050124. [DOI] [PubMed] [Google Scholar]
- Cabel M., Meiselman H. J., Popel A. S., Johnson P. C. Contribution of red blood cell aggregation to venous vascular resistance in skeletal muscle. Am J Physiol. 1997 Feb;272(2 Pt 2):H1020–H1032. doi: 10.1152/ajpheart.1997.272.2.H1020. [DOI] [PubMed] [Google Scholar]
- Chen S., Gavish B., Zhang S., Mahler Y., Yedgar S. Monitoring of erythrocyte aggregate morphology under flow by computerized image analysis. Biorheology. 1995 Jul-Aug;32(4):487–496. doi: 10.1016/0006-355X(95)00025-5. [DOI] [PubMed] [Google Scholar]
- Das B., Enden G., Popel A. S. Stratified multiphase model for blood flow in a venular bifurcation. Ann Biomed Eng. 1997 Jan-Feb;25(1):135–153. doi: 10.1007/BF02738545. [DOI] [PubMed] [Google Scholar]
- Kernick D., Jay A. W., Rowlands S., Skibo L. Experiments on Rouleu formation. Can J Physiol Pharmacol. 1973 Sep;51(9):690–699. doi: 10.1139/y73-105. [DOI] [PubMed] [Google Scholar]
- Knisely M. H., Bloch E. H., Eliot T. S., Warner L. Sludged Blood. Science. 1947 Nov 7;106(2758):431–440. doi: 10.1126/science.106.2758.431. [DOI] [PubMed] [Google Scholar]
- Samsel R. W., Perelson A. S. Kinetics of rouleau formation. I. A mass action approach with geometric features. Biophys J. 1982 Feb;37(2):493–514. doi: 10.1016/S0006-3495(82)84696-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shiga T., Imaizumi K., Harada N., Sekiya M. Kinetics of rouleaux formation using TV image analyzer. I. Human erythrocytes. Am J Physiol. 1983 Aug;245(2):H252–H258. doi: 10.1152/ajpheart.1983.245.2.H252. [DOI] [PubMed] [Google Scholar]