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. 2000 Aug;79(2):1107–1118. doi: 10.1016/S0006-3495(00)76364-8

Atomic force microscope image contrast mechanisms on supported lipid bilayers.

J Schneider 1, Y F Dufrêne 1, W R Barger Jr 1, G U Lee 1
PMCID: PMC1301006  PMID: 10920040

Abstract

This work presents a methodology to measure and quantitatively interpret force curves on supported lipid bilayers in water. We then use this method to correlate topographic imaging contrast in atomic force microscopy (AFM) images of phase-separated Langmuir-Blodgett bilayers with imaging load. Force curves collected on pure monolayers of both distearoylphosphatidylethanolamine (DSPE) and monogalactosylethanolamine (MGDG) and dioleoylethanolamine (DOPE) deposited at similar surface pressures onto a monolayer of DSPE show an abrupt breakthrough event at a repeatable, material-dependent force. The breakthrough force for DSPE and MGDG is sizable, whereas the breakthrough force for DOPE is too small to measure accurately. Contact-mode AFM images on 1:1 mixed monolayers of DSPE/DOPE and MGDG/DOPE have a high topographic contrast at loads between the breakthrough force of each phase, and a low topographic contrast at loads above the breakthrough force of both phases. Frictional contrast is inverted and magnified at loads above the breakthrough force of both phases. These results emphasize the important role that surface forces and mechanics can play in imaging multicomponent biomembranes with AFM.

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Selected References

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  1. Berridge M. J., Irvine R. F. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984 Nov 22;312(5992):315–321. doi: 10.1038/312315a0. [DOI] [PubMed] [Google Scholar]
  2. Blackman GS, Mate CM, Philpott MR. Interaction forces of a sharp tungsten tip with molecular films on silicon surfaces. Phys Rev Lett. 1990 Oct 29;65(18):2270–2273. doi: 10.1103/PhysRevLett.65.2270. [DOI] [PubMed] [Google Scholar]
  3. Butt H. J. Electrostatic interaction in atomic force microscopy. Biophys J. 1991 Oct;60(4):777–785. doi: 10.1016/S0006-3495(91)82112-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Butt H. J. Measuring electrostatic, van der Waals, and hydration forces in electrolyte solutions with an atomic force microscope. Biophys J. 1991 Dec;60(6):1438–1444. doi: 10.1016/S0006-3495(91)82180-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chi L. F., Anders M., Fuchs H., Johnston R. R., Ringsdorf H. Domain structures in langmuir-blodgett films investigated by atomic force microscopy. Science. 1993 Jan 8;259(5092):213–216. doi: 10.1126/science.259.5092.213. [DOI] [PubMed] [Google Scholar]
  6. Cornell B. A., Braach-Maksvytis V. L., King L. G., Osman P. D., Raguse B., Wieczorek L., Pace R. J. A biosensor that uses ion-channel switches. Nature. 1997 Jun 5;387(6633):580–583. doi: 10.1038/42432. [DOI] [PubMed] [Google Scholar]
  7. Discher B. M., Maloney K. M., Schief W. R., Jr, Grainger D. W., Vogel V., Hall S. B. Lateral phase separation in interfacial films of pulmonary surfactant. Biophys J. 1996 Nov;71(5):2583–2590. doi: 10.1016/S0006-3495(96)79450-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dufrêne Y. F., Boland T., Schneider J. W., Barger W. R., Lee G. U. Characterization of the physical properties of model biomembranes at the nanometer scale with the atomic force microscope. Faraday Discuss. 1998;(111):79–157. doi: 10.1039/a807637e. [DOI] [PubMed] [Google Scholar]
  9. Helm C. A., Israelachvili J. N., McGuiggan P. M. Molecular mechanisms and forces involved in the adhesion and fusion of amphiphilic bilayers. Science. 1989 Nov 17;246(4932):919–922. doi: 10.1126/science.2814514. [DOI] [PubMed] [Google Scholar]
  10. Helm C. A., Israelachvili J. N., McGuiggan P. M. Role of hydrophobic forces in bilayer adhesion and fusion. Biochemistry. 1992 Feb 18;31(6):1794–1805. doi: 10.1021/bi00121a030. [DOI] [PubMed] [Google Scholar]
  11. Ho R., Yuan J. Y., Shao Z. Hydration force in the atomic force microscope: A computational study. Biophys J. 1998 Aug;75(2):1076–1083. doi: 10.1016/S0006-3495(98)77597-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hui S. W., Viswanathan R., Zasadzinski J. A., Israelachvili J. N. The structure and stability of phospholipid bilayers by atomic force microscopy. Biophys J. 1995 Jan;68(1):171–178. doi: 10.1016/S0006-3495(95)80172-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lipp M. M., Lee K. Y., Waring A., Zasadzinski J. A. Fluorescence, polarized fluorescence, and Brewster angle microscopy of palmitic acid and lung surfactant protein B monolayers. Biophys J. 1997 Jun;72(6):2783–2804. doi: 10.1016/S0006-3495(97)78921-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marra J., Israelachvili J. Direct measurements of forces between phosphatidylcholine and phosphatidylethanolamine bilayers in aqueous electrolyte solutions. Biochemistry. 1985 Aug 13;24(17):4608–4618. doi: 10.1021/bi00338a020. [DOI] [PubMed] [Google Scholar]
  15. McConnell H. M., Watts T. H., Weis R. M., Brian A. A. Supported planar membranes in studies of cell-cell recognition in the immune system. Biochim Biophys Acta. 1986 Jun 12;864(1):95–106. doi: 10.1016/0304-4157(86)90016-x. [DOI] [PubMed] [Google Scholar]
  16. Radmacher M., Fritz M., Hansma P. K. Imaging soft samples with the atomic force microscope: gelatin in water and propanol. Biophys J. 1995 Jul;69(1):264–270. doi: 10.1016/S0006-3495(95)79897-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sackmann E. Supported membranes: scientific and practical applications. Science. 1996 Jan 5;271(5245):43–48. doi: 10.1126/science.271.5245.43. [DOI] [PubMed] [Google Scholar]
  18. Schwartz D. K., Garnaes J., Viswanathan R., Zasadzinski J. A. Surface order and stability of langmuir-blodgett films. Science. 1992 Jul 24;257(5069):508–511. doi: 10.1126/science.257.5069.508. [DOI] [PubMed] [Google Scholar]
  19. Seddon J. M., Cevc G., Kaye R. D., Marsh D. X-ray diffraction study of the polymorphism of hydrated diacyl- and dialkylphosphatidylethanolamines. Biochemistry. 1984 Jun 5;23(12):2634–2644. doi: 10.1021/bi00307a015. [DOI] [PubMed] [Google Scholar]
  20. Sheiko SS, Möller M, Reuvekamp EM, Zandbergen HW. Calibration and evaluation of scanning-force-microscopy probes. Phys Rev B Condens Matter. 1993 Aug 15;48(8):5675–5678. doi: 10.1103/physrevb.48.5675. [DOI] [PubMed] [Google Scholar]
  21. Yu Y. C., Pakalns T., Dori Y., McCarthy J. B., Tirrell M., Fields G. B. Construction of biologically active protein molecular architecture using self-assembling peptide-amphiphiles. Methods Enzymol. 1997;289:571–587. doi: 10.1016/s0076-6879(97)89065-9. [DOI] [PubMed] [Google Scholar]
  22. Zasadzinski J. A., Helm C. A., Longo M. L., Weisenhorn A. L., Gould S. A., Hansma P. K. Atomic force microscopy of hydrated phosphatidylethanolamine bilayers. Biophys J. 1991 Mar;59(3):755–760. doi: 10.1016/S0006-3495(91)82288-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. ten Grotenhuis E., Demel R. A., Ponec M., Boer D. R., van Miltenburg J. C., Bouwstra J. A. Phase behavior of stratum corneum lipids in mixed Langmuir-Blodgett monolayers. Biophys J. 1996 Sep;71(3):1389–1399. doi: 10.1016/S0006-3495(96)79341-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. von Nahmen A., Schenk M., Sieber M., Amrein M. The structure of a model pulmonary surfactant as revealed by scanning force microscopy. Biophys J. 1997 Jan;72(1):463–469. doi: 10.1016/S0006-3495(97)78687-9. [DOI] [PMC free article] [PubMed] [Google Scholar]

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