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. 2016 Apr 13;16(4):559–568. doi: 10.3233/CBM-160598

New miRNA expression abnormalities in laryngeal squamous cell carcinoma

Magdalena Cybula a,*, Ƚukasz Wieteska a, Magdalena Józefowicz-Korczyńska b, Michaƚ Seweryn Karbownik c, Weronika Lucas Grzelczyk b, Janusz Szemraj a
PMCID: PMC13016522  PMID: 27002759

Abstract

BACKGROUND:

Although the development of novel diagnostic and treatment strategies concerning laryngeal cancer is highly intensive, the survival rate remains virtually unchanged. Small non-coding RNAs appear to be very promising biomarkers - and so remain the focus of extensive investigation in laryngeal cancer.

OBJECTIVE:

We examined the expression of five miRNA and five genes related to cancer whether they could be potential laryngeal cancer biomarkers.

METHODS:

We performed an analysis in 47 patients diagnosed with laryngeal cancer. The qPCR technique was used to investigate the expression profile.

RESULTS:

While miR-21-3p and miR-525-5p were found to be significantly up-regulated, miR-139-3p and miR-885-5p expression is lower in laryngeal cancer. Moreover, PIK3R1 and HACE1 were found to be also down-regulated.

CONCLUSIONS:

The change in miRNA expression is frequent than the expression of other tested genes. The expression of passenger strands such as miR-21-3p and miR-139-3p, which are rarely investigated, is also significantly affected in laryngeal cancer. While PIK3R1, HACE1, miR-139-3p, and miR-885-5p may act as tumor suppressor genes in the studied tumour type, miR-21-3p and miR-525-5p seem to have oncogenic properties. Our findings suggest that miR-885-5p and PIK3R1 are the best indicators for the classification of laryngeal cancer tissue and normal mucosa.

Keywords: Laryngeal squamous cell carcinoma, miRNA, expression

Full Text

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