Table 1.

Primers, PCR conditions, restriction enzyme and digested condition for PCR-RFLP

SNPs	Primers (5'−3')	PCR Conditions	RE	Expected Fragments (bp)
ERCC4(rs6498486)	FP: TTTTTGGCAGCTTGAGGCTA RP: CTCAGAAAGCCGAAGAGAGC	94oC for 5 m 30s 60oC for 30s 72oC for 5m 30s	MnlI (MnLI)	NH (AA): 204 HZ (AC): 51, 153, 204 VH (CC): 51, 153

Here, FP Forward primer, RP Reverse primer; RE Restriction enzyme, NH Normal homozygote, HZ Heterozygote, VH Variant homozygote