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. 2002 May;82(5):2487–2498. doi: 10.1016/s0006-3495(02)75591-4

SANS study on the effect of lanthanide ions and charged lipids on the morphology of phospholipid mixtures. Small-angle neutron scattering.

Mu-Ping Nieh 1, Charles J Glinka 1, Susan Krueger 1, R Scott Prosser 1, John Katsaras 1
PMCID: PMC1302038  PMID: 11964236

Abstract

The structural phase behavior of phospholipid mixtures consisting of short-chain (dihexanoyl phosphatidylcholine) and long-chain lipids (dimyristoyl phosphatidylcholine and dimyristoyl phosphatidylglycerol), with and without lanthanide ions was investigated by small-angle neutron scattering (SANS). SANS profiles were obtained from 10 degrees C to 55 degrees C using lipid concentrations ranging from 0.0025 g/ml to 0.25 g/ml. The results reveal a wealth of distinct morphologies, including lamellae, multi-lamellar vesicles, unilamellar vesicles, and bicellar disks.

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Selected References

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  1. Bax A., Tjandra N. High-resolution heteronuclear NMR of human ubiquitin in an aqueous liquid crystalline medium. J Biomol NMR. 1997 Oct;10(3):289–292. doi: 10.1023/a:1018308717741. [DOI] [PubMed] [Google Scholar]
  2. Brunner E., Arnold M. R., Kremer W., Kalbitzer H. R. Pressure-stability of phospholipid bicelles: measurement of residual dipolar couplings under extreme conditions. J Biomol NMR. 2001 Oct;21(2):173–176. doi: 10.1023/a:1012420618417. [DOI] [PubMed] [Google Scholar]
  3. Caffrey M., Hogan J., Mencke A. Kinetics of the barotropic ripple (P beta')/lamellar liquid crystal (L alpha) phase transition in fully hydrated dimyristoylphosphatidylcholine (DMPC) monitored by time-resolved x-ray diffraction. Biophys J. 1991 Aug;60(2):456–466. doi: 10.1016/S0006-3495(91)82072-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glover K. J., Whiles J. A., Wu G., Yu N., Deems R., Struppe J. O., Stark R. E., Komives E. A., Vold R. R. Structural evaluation of phospholipid bicelles for solution-state studies of membrane-associated biomolecules. Biophys J. 2001 Oct;81(4):2163–2171. doi: 10.1016/s0006-3495(01)75864-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hare B. J., Prestegard J. H., Engelman D. M. Small angle x-ray scattering studies of magnetically oriented lipid bilayers. Biophys J. 1995 Nov;69(5):1891–1896. doi: 10.1016/S0006-3495(95)80059-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laggner P. X-ray diffraction on biomembranes with emphasis on lipid moiety. Subcell Biochem. 1994;23:451–491. doi: 10.1007/978-1-4615-1863-1_11. [DOI] [PubMed] [Google Scholar]
  7. Lohner K., Staudegger E., Prenner E. J., Lewis R. N., Kriechbaum M., Degovics G., McElhaney R. N. Effect of staphylococcal delta-lysin on the thermotropic phase behavior and vesicle morphology of dimyristoylphosphatidylcholine lipid bilayer model membranes. Differential scanning calorimetric, 31P nuclear magnetic resonance and Fourier transform infrared spectroscopic, and X-ray diffraction studies. Biochemistry. 1999 Dec 14;38(50):16514–16528. doi: 10.1021/bi9913101. [DOI] [PubMed] [Google Scholar]
  8. Losonczi J. A., Prestegard J. H. Improved dilute bicelle solutions for high-resolution NMR of biological macromolecules. J Biomol NMR. 1998 Oct;12(3):447–451. doi: 10.1023/a:1008302110884. [DOI] [PubMed] [Google Scholar]
  9. Luchette P. A., Vetman T. N., Prosser R. S., Hancock R. E., Nieh M. P., Glinka C. J., Krueger S., Katsaras J. Morphology of fast-tumbling bicelles: a small angle neutron scattering and NMR study. Biochim Biophys Acta. 2001 Aug 6;1513(2):83–94. doi: 10.1016/s0005-2736(01)00358-3. [DOI] [PubMed] [Google Scholar]
  10. Meuse C. W., Krueger S., Majkrzak C. F., Dura J. A., Fu J., Connor J. T., Plant A. L. Hybrid bilayer membranes in air and water: infrared spectroscopy and neutron reflectivity studies. Biophys J. 1998 Mar;74(3):1388–1398. doi: 10.1016/S0006-3495(98)77851-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ottiger M., Bax A. Characterization of magnetically oriented phospholipid micelles for measurement of dipolar couplings in macromolecules. J Biomol NMR. 1998 Oct;12(3):361–372. doi: 10.1023/a:1008366116644. [DOI] [PubMed] [Google Scholar]
  12. Prosser R. S., Bryant H., Bryant R. G., Vold R. R. Lanthanide chelates as bilayer alignment tools in NMR studies of membrane-associated peptides. J Magn Reson. 1999 Dec;141(2):256–260. doi: 10.1006/jmre.1999.1855. [DOI] [PubMed] [Google Scholar]
  13. Prosser R. S., Hwang J. S., Vold R. R. Magnetically aligned phospholipid bilayers with positive ordering: a new model membrane system. Biophys J. 1998 May;74(5):2405–2418. doi: 10.1016/S0006-3495(98)77949-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Prosser R. S., Volkov V. B., Shiyanovskaya I. V. Novel chelate-induced magnetic alignment of biological membranes. Biophys J. 1998 Nov;75(5):2163–2169. doi: 10.1016/S0006-3495(98)77659-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Prosser R. S., Volkov V. B., Shiyanovskaya I. V. Solid-state NMR studies of magnetically aligned phospholipid membranes: taming lanthanides for membrane protein studies. Biochem Cell Biol. 1998;76(2-3):443–451. doi: 10.1139/bcb-76-2-3-443. [DOI] [PubMed] [Google Scholar]
  16. Sanders C. R., 2nd, Landis G. C. Reconstitution of membrane proteins into lipid-rich bilayered mixed micelles for NMR studies. Biochemistry. 1995 Mar 28;34(12):4030–4040. doi: 10.1021/bi00012a022. [DOI] [PubMed] [Google Scholar]
  17. Sanders C. R., 2nd, Schwonek J. P. Characterization of magnetically orientable bilayers in mixtures of dihexanoylphosphatidylcholine and dimyristoylphosphatidylcholine by solid-state NMR. Biochemistry. 1992 Sep 22;31(37):8898–8905. doi: 10.1021/bi00152a029. [DOI] [PubMed] [Google Scholar]
  18. Sanders C. R., Prosser R. S. Bicelles: a model membrane system for all seasons? Structure. 1998 Oct 15;6(10):1227–1234. doi: 10.1016/s0969-2126(98)00123-3. [DOI] [PubMed] [Google Scholar]
  19. Struppe J., Vold R. R. Dilute bicellar solutions for structural NMR work. J Magn Reson. 1998 Dec;135(2):541–546. doi: 10.1006/jmre.1998.1605. [DOI] [PubMed] [Google Scholar]
  20. Ulrich A. S., Watts A. 2H NMR lineshapes of immobilized uniaxially oriented membrane proteins. Solid State Nucl Magn Reson. 1993 Apr;2(1-2):21–36. doi: 10.1016/0926-2040(93)90060-z. [DOI] [PubMed] [Google Scholar]
  21. Vold R. R., Prosser R. S., Deese A. J. Isotropic solutions of phospholipid bicelles: a new membrane mimetic for high-resolution NMR studies of polypeptides. J Biomol NMR. 1997 Apr;9(3):329–335. doi: 10.1023/a:1018643312309. [DOI] [PubMed] [Google Scholar]

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