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. 2002 Jan;82(1 Pt 1):481–492. doi: 10.1016/S0006-3495(02)75413-1

Functional imaging of plants: a nuclear magnetic resonance study of a cucumber plant.

Tom Scheenen 1, Anneriet Heemskerk 1, Andrie de Jager 1, Frank Vergeldt 1, Henk Van As 1
PMCID: PMC1302488  PMID: 11751335

Abstract

Functional magnetic resonance imaging was used to study transients of biophysical parameters in a cucumber plant in response to environmental changes. Detailed flow imaging experiments showed the location of xylem and phloem in the stem and the response of the following flow characteristics to the imposed environmental changes: the total amount of water, the amount of stationary and flowing water, the linear velocity of the flowing water, and the volume flow. The total measured volume flow through the plant stem was in good agreement with the independently measured water uptake by the roots. A separate analysis of the flow characteristics for two vascular bundles revealed that changes in volume flow of the xylem sap were accounted for by a change in linear-flow velocities in the xylem vessels. Multiple-spin echo experiments revealed two water fractions for different tissues in the plant stem; the spin-spin relaxation time of the larger fraction of parenchyma tissue in the center of the stem and the vascular tissue was down by 17% in the period after cooling the roots of the plant. This could point to an increased water permeability of the tonoplast membrane of the observed cells in this period of quick recovery from severe water loss.

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Selected References

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  1. Callaghan P. T., Clark C. J., Forde L. C. Use of static and dynamic NMR microscopy to investigate the origins of contrast in images of biological tissues. Biophys Chem. 1994 May;50(1-2):225–235. doi: 10.1016/0301-4622(94)85034-8. [DOI] [PubMed] [Google Scholar]
  2. Donker H. C., Van As H., Snijder H. J., Edzes H. T. Quantitative 1H-NMR imaging of water in white button mushrooms (Agaricus bisporus). Magn Reson Imaging. 1997;15(1):113–121. doi: 10.1016/s0730-725x(96)00328-1. [DOI] [PubMed] [Google Scholar]
  3. Edzes H. T., van Dusschoten D., Van As H. Quantitative T2 imaging of plant tissues by means of multi-echo MRI microscopy. Magn Reson Imaging. 1998;16(2):185–196. doi: 10.1016/s0730-725x(97)00274-9. [DOI] [PubMed] [Google Scholar]
  4. Hennig J., Nauerth A., Friedburg H. RARE imaging: a fast imaging method for clinical MR. Magn Reson Med. 1986 Dec;3(6):823–833. doi: 10.1002/mrm.1910030602. [DOI] [PubMed] [Google Scholar]
  5. Hills B. P., Duce S. L. The influence of chemical and diffusive exchange on water proton transverse relaxation in plant tissues. Magn Reson Imaging. 1990;8(3):321–331. doi: 10.1016/0730-725x(90)90106-c. [DOI] [PubMed] [Google Scholar]
  6. Kuchenbrod E., Kahler E., Thürmer F., Deichmann R., Zimmermann U., Haase A. Functional magnetic resonance imaging in intact plants--quantitative observation of flow in plant vessels. Magn Reson Imaging. 1998 Apr;16(3):331–338. doi: 10.1016/s0730-725x(97)00307-x. [DOI] [PubMed] [Google Scholar]
  7. Rokitta M., Zimmermann U., Haase A. Fast NMR flow measurements in plants using FLASH imaging. J Magn Reson. 1999 Mar;137(1):29–32. doi: 10.1006/jmre.1998.1611. [DOI] [PubMed] [Google Scholar]
  8. Schaafsma T. J., Van As H., Palstra W. D., Snaar J. E., de Jager P. A. Quantitative measurement and imaging of transport processes in plants and porous media by 1H NMR. Magn Reson Imaging. 1992;10(5):827–836. doi: 10.1016/0730-725x(92)90418-y. [DOI] [PubMed] [Google Scholar]
  9. Scheenen T. W., Vergeldt F. J., Windt C. W., de Jager P. A., Van As H. Microscopic imaging of slow flow and diffusion: a pulsed field gradient stimulated echo sequence combined with turbo spin echo imaging. J Magn Reson. 2001 Jul;151(1):94–100. doi: 10.1006/jmre.2001.2362. [DOI] [PubMed] [Google Scholar]
  10. Scheenen T. W., van Dusschoten D., de Jager P. A., Van As H. Microscopic displacement imaging with pulsed field gradient turbo spin-echo NMR. J Magn Reson. 2000 Feb;142(2):207–215. doi: 10.1006/jmre.1999.1916. [DOI] [PubMed] [Google Scholar]
  11. Scheenen T. W., van Dusschoten D., de Jager P. A., Van As H. Quantification of water transport in plants with NMR imaging. J Exp Bot. 2000 Oct;51(351):1751–1759. doi: 10.1093/jexbot/51.351.1751. [DOI] [PubMed] [Google Scholar]
  12. Snaar J. E., Van As H. Probing water compartments and membrane permeability in plant cells by H NMR relaxation measurements. Biophys J. 1992 Dec;63(6):1654–1658. doi: 10.1016/S0006-3495(92)81741-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. van As H., Schaafsma T. J. Noninvasive measurement of plant water flow by nuclear magnetic resonance. Biophys J. 1984 Feb;45(2):469–472. doi: 10.1016/S0006-3495(84)84170-3. [DOI] [PMC free article] [PubMed] [Google Scholar]

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