Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1998 Feb;74(2 Pt 1):857–868. doi: 10.1016/S0006-3495(98)74009-3

Measurement of the lateral diffusion of dipalmitoylphosphatidylcholine adsorbed on silica beads in the absence and presence of melittin: a 31P two-dimensional exchange solid-state NMR study.

F Picard 1, M J Paquet 1, E J Dufourc 1, M Auger 1
PMCID: PMC1302565  PMID: 9533697

Abstract

31P two-dimensional exchange solid-state NMR spectroscopy was used to measure the lateral diffusion, D(L), in the fluid phase of dipalmitoylphosphatidylcholine (DPPC) in the presence and absence of melittin. The use of a spherical solid support with a radius of 320 +/- 20 nm, on which lipids and peptides are adsorbed together, and a novel way of analyzing the two-dimensional exchange patterns afforded a narrow distribution of D(L) centered at a value of (8.8 +/- 0.5) x 10(-8) cm2/s for the pure lipid system and a large distribution of D(L) spanning 1 x 10(-8) to 10 x 10(-8) cm2/s for the lipids in the presence of melittin. In addition, the determination of D(L) for nonsupported DPPC multilamellar vesicles (MLVs) suggests that the support does not slow down the lipid diffusion and that the radii of the bilayers vary from 300 to 800 nm. Finally, the DPPC-melittin complex is stabilized at the surface of the silica beads in the gel phase, opening the way to further study of the interaction between melittin and DPPC.

Full Text

The Full Text of this article is available as a PDF (157.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auger M., Smith I. C., Jarrell H. C. Slow motions in lipid bilayers. Direct detection by two-dimensional solid-state deuterium nuclear magnetic resonance. Biophys J. 1991 Jan;59(1):31–38. doi: 10.1016/S0006-3495(91)82195-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bayerl T. M., Bloom M. Physical properties of single phospholipid bilayers adsorbed to micro glass beads. A new vesicular model system studied by 2H-nuclear magnetic resonance. Biophys J. 1990 Aug;58(2):357–362. doi: 10.1016/S0006-3495(90)82382-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brumm T., Möps A., Dolainsky C., Brückner S., Bayerl T. M. Macroscopic orientation effects in broadline NMR-spectra of model membranes at high magnetic field strength: A method preventing such effects. Biophys J. 1992 Apr;61(4):1018–1024. doi: 10.1016/S0006-3495(92)81909-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cullis P. R. Lateral diffusion rates of phosphatidylcholine in vesicle membranes: effects of cholesterol and hydrocarbon phase transitions. FEBS Lett. 1976 Nov;70(1):223–228. doi: 10.1016/0014-5793(76)80762-4. [DOI] [PubMed] [Google Scholar]
  5. Dasseux J. L., Faucon J. F., Lafleur M., Pezolet M., Dufourcq J. A restatement of melittin-induced effects on the thermotropism of zwitterionic phospholipids. Biochim Biophys Acta. 1984 Aug 8;775(1):37–50. doi: 10.1016/0005-2736(84)90232-3. [DOI] [PubMed] [Google Scholar]
  6. Davis J. H. The description of membrane lipid conformation, order and dynamics by 2H-NMR. Biochim Biophys Acta. 1983 Mar 21;737(1):117–171. doi: 10.1016/0304-4157(83)90015-1. [DOI] [PubMed] [Google Scholar]
  7. Dempsey C. E. The actions of melittin on membranes. Biochim Biophys Acta. 1990 May 7;1031(2):143–161. doi: 10.1016/0304-4157(90)90006-x. [DOI] [PubMed] [Google Scholar]
  8. Dolainsky C, Unger M, Bloom M, Bayerl TM. Two-dimensional exchange 2H NMR experiments of phospholipid bilayers on a spherical solid support. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1995 May;51(5):4743–4750. doi: 10.1103/physreve.51.4743. [DOI] [PubMed] [Google Scholar]
  9. Dufourc E. J., Bonmatin J. M., Dufourcq J. Membrane structure and dynamics by 2H- and 31P-NMR. Effects of amphipatic peptidic toxins on phospholipid and biological membranes. Biochimie. 1989 Jan;71(1):117–123. doi: 10.1016/0300-9084(89)90141-7. [DOI] [PubMed] [Google Scholar]
  10. Dufourc E. J., Smith I. C., Dufourcq J. Molecular details of melittin-induced lysis of phospholipid membranes as revealed by deuterium and phosphorus NMR. Biochemistry. 1986 Oct 21;25(21):6448–6455. doi: 10.1021/bi00369a016. [DOI] [PubMed] [Google Scholar]
  11. Dufourcq J., Faucon J. F., Fourche G., Dasseux J. L., Le Maire M., Gulik-Krzywicki T. Morphological changes of phosphatidylcholine bilayers induced by melittin: vesicularization, fusion, discoidal particles. Biochim Biophys Acta. 1986 Jul 10;859(1):33–48. doi: 10.1016/0005-2736(86)90315-9. [DOI] [PubMed] [Google Scholar]
  12. Désormeaux A., Laroche G., Bougis P. E., Pézolet M. Characterization by infrared spectroscopy of the interaction of a cardiotoxin with phosphatidic acid and with binary mixtures of phosphatidic acid and phosphatidylcholine. Biochemistry. 1992 Dec 8;31(48):12173–12182. doi: 10.1021/bi00163a029. [DOI] [PubMed] [Google Scholar]
  13. Faucon J. F., Bonmatin J. M., Dufourcq J., Dufourc E. J. Acyl chain length dependence in the stability of melittin-phosphatidylcholine complexes. A light scattering and 31P-NMR study. Biochim Biophys Acta. 1995 Mar 22;1234(2):235–243. doi: 10.1016/0005-2736(94)00298-4. [DOI] [PubMed] [Google Scholar]
  14. Fenske D. B., Cullis P. R. Chemical exchange between lamellar and non-lamellar lipid phases. A one- and two-dimensional 31P-NMR study. Biochim Biophys Acta. 1992 Jul 27;1108(2):201–209. doi: 10.1016/0005-2736(92)90026-i. [DOI] [PubMed] [Google Scholar]
  15. Fenske D. B., Jarrell H. C. Phosphorus-31 two-dimensional solid-state exchange NMR. Application to model membrane and biological systems. Biophys J. 1991 Jan;59(1):55–69. doi: 10.1016/S0006-3495(91)82198-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Fenske D. B., Letellier M., Roy R., Smith I. C., Jarrell H. C. Effect of calcium on the dynamic behavior of sialylglycerolipids and phospholipids in mixed model membranes. A 2H and 31P NMR study. Biochemistry. 1991 Oct 29;30(43):10542–10550. doi: 10.1021/bi00107a025. [DOI] [PubMed] [Google Scholar]
  17. Fringeli U. P., Günthard H. H. Infrared membrane spectroscopy. Mol Biol Biochem Biophys. 1981;31:270–332. doi: 10.1007/978-3-642-81537-9_6. [DOI] [PubMed] [Google Scholar]
  18. Griffin R. G. Solid state nuclear magnetic resonance of lipid bilayers. Methods Enzymol. 1981;72:108–174. doi: 10.1016/s0076-6879(81)72010-x. [DOI] [PubMed] [Google Scholar]
  19. Johnson S. J., Bayerl T. M., McDermott D. C., Adam G. W., Rennie A. R., Thomas R. K., Sackmann E. Structure of an adsorbed dimyristoylphosphatidylcholine bilayer measured with specular reflection of neutrons. Biophys J. 1991 Feb;59(2):289–294. doi: 10.1016/S0006-3495(91)82222-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kapitza H. G., Rüppel D. A., Galla H. J., Sackmann E. Lateral diffusion of lipids and glycophorin in solid phosphatidylcholine bilayers. The role of structural defects. Biophys J. 1984 Mar;45(3):577–587. doi: 10.1016/S0006-3495(84)84195-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Karakatsanis P, Bayerl TM. Diffusion measurements in oriented phospholipid bilayers by 1H-NMR in a static fringe field gradient. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1996 Aug;54(2):1785–1790. doi: 10.1103/physreve.54.1785. [DOI] [PubMed] [Google Scholar]
  22. Katsu T., Kuroko M., Morikawa T., Sanchika K., Fujita Y., Yamamura H., Uda M. Mechanism of membrane damage induced by the amphipathic peptides gramicidin S and melittin. Biochim Biophys Acta. 1989 Aug 7;983(2):135–141. doi: 10.1016/0005-2736(89)90226-5. [DOI] [PubMed] [Google Scholar]
  23. Katsu T., Ninomiya C., Kuroko M., Kobayashi H., Hirota T., Fujita Y. Action mechanism of amphipathic peptides gramicidin S and melittin on erythrocyte membrane. Biochim Biophys Acta. 1988 Mar 22;939(1):57–63. doi: 10.1016/0005-2736(88)90047-8. [DOI] [PubMed] [Google Scholar]
  24. Köchy T, Bayerl TM. Lateral diffusion coefficients of phospholipids in spherical bilayers on a solid support measured by 2H-nuclear-magnetic-resonance relaxation. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1993 Mar;47(3):2109–2116. doi: 10.1103/physreve.47.2109. [DOI] [PubMed] [Google Scholar]
  25. Lee B. S., Mabry S. A., Jonas A., Jonas J. High-pressure proton NMR study of lateral self-diffusion of phosphatidylcholines in sonicated unilamellar vesicles. Chem Phys Lipids. 1995 Nov 17;78(2):103–117. doi: 10.1016/0009-3084(95)02493-3. [DOI] [PubMed] [Google Scholar]
  26. Lis L. J., McAlister M., Fuller N., Rand R. P., Parsegian V. A. Interactions between neutral phospholipid bilayer membranes. Biophys J. 1982 Mar;37(3):657–665. [PMC free article] [PubMed] [Google Scholar]
  27. Macquaire F, Bloom M. Membrane curvature studied using two-dimensional NMR in fluid lipid bilayers. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1995 May;51(5):4735–4742. doi: 10.1103/physreve.51.4735. [DOI] [PubMed] [Google Scholar]
  28. Nabet A., Boggs J. M., Pézolet M. Study by infrared spectroscopy of the interaction of bovine myelin basic protein with phosphatidic acid. Biochemistry. 1994 Dec 13;33(49):14792–14799. doi: 10.1021/bi00253a018. [DOI] [PubMed] [Google Scholar]
  29. Naumann C., Brumm T., Bayerl T. M. Phase transition behavior of single phosphatidylcholine bilayers on a solid spherical support studied by DSC, NMR and FT-IR. Biophys J. 1992 Nov;63(5):1314–1319. doi: 10.1016/S0006-3495(92)81708-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Pott T., Dufourc E. J. Action of melittin on the DPPC-cholesterol liquid-ordered phase: a solid state 2H-and 31P-NMR study. Biophys J. 1995 Mar;68(3):965–977. doi: 10.1016/S0006-3495(95)80272-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Reinl H. M., Bayerl T. M. Interaction of myelin basic protein with single bilayers on a solid support: an NMR, DSC and polarized infrared ATR study. Biochim Biophys Acta. 1993 Sep 19;1151(2):127–136. doi: 10.1016/0005-2736(93)90095-h. [DOI] [PubMed] [Google Scholar]
  32. Seelig J. 31P nuclear magnetic resonance and the head group structure of phospholipids in membranes. Biochim Biophys Acta. 1978 Jul 31;515(2):105–140. doi: 10.1016/0304-4157(78)90001-1. [DOI] [PubMed] [Google Scholar]
  33. Seelig J. Deuterium magnetic resonance: theory and application to lipid membranes. Q Rev Biophys. 1977 Aug;10(3):353–418. doi: 10.1017/s0033583500002948. [DOI] [PubMed] [Google Scholar]
  34. Singer S. J., Nicolson G. L. The fluid mosaic model of the structure of cell membranes. Science. 1972 Feb 18;175(4023):720–731. doi: 10.1126/science.175.4023.720. [DOI] [PubMed] [Google Scholar]
  35. Tabony J., Perly B. Quasielastic neutron scattering measurements of fast local translational diffusion of lipid molecules in phospholipid bilayers. Biochim Biophys Acta. 1991 Mar 18;1063(1):67–72. doi: 10.1016/0005-2736(91)90354-b. [DOI] [PubMed] [Google Scholar]
  36. Tamm L. K., McConnell H. M. Supported phospholipid bilayers. Biophys J. 1985 Jan;47(1):105–113. doi: 10.1016/S0006-3495(85)83882-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Tocanne J. F., Dupou-Cézanne L., Lopez A., Tournier J. F. Lipid lateral diffusion and membrane organization. FEBS Lett. 1989 Oct 23;257(1):10–16. doi: 10.1016/0014-5793(89)81774-0. [DOI] [PubMed] [Google Scholar]
  38. Vaz W. L., Almeida P. F. Microscopic versus macroscopic diffusion in one-component fluid phase lipid bilayer membranes. Biophys J. 1991 Dec;60(6):1553–1554. doi: 10.1016/S0006-3495(91)82190-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Vaz W. L., Clegg R. M., Hallmann D. Translational diffusion of lipids in liquid crystalline phase phosphatidylcholine multibilayers. A comparison of experiment with theory. Biochemistry. 1985 Jan 29;24(3):781–786. doi: 10.1021/bi00324a037. [DOI] [PubMed] [Google Scholar]
  40. Wennerström H., Lindblom G. Biological and model membranes studied by nuclear magnetic resonance of spin one half nuclei. Q Rev Biophys. 1977 Feb;10(1):67–96. doi: 10.1017/s0033583500000147. [DOI] [PubMed] [Google Scholar]
  41. de Wolf F. A., Maliepaard M., van Dorsten F., Berghuis I., Nicolay K., de Kruijff B. Comparable interaction of doxorubicin with various acidic phospholipids results in changes of lipid order and dynamics. Biochim Biophys Acta. 1990 Nov 14;1096(1):67–80. doi: 10.1016/0925-4439(90)90014-g. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES