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Zal, Tomasz, and Nicholas R. J. Gascoigne. Photobleaching-corrected FRET efficiency imaging of live cells. Biophys. J. 86: 3923–3939.
Due to a typesetting error, Eq. 15 did not print correctly.
The correct equation is below:
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The parameter G is the ratio of the decrease of cross-talk corrected sensitized fluorescence in the IDA filter set to the increase of the donor fluorescence in the IDD filter set after photobleaching of acceptor.
