Skip to main content
PLOS One logoLink to PLOS One
. 2026 Apr 3;21(4):e0328143. doi: 10.1371/journal.pone.0328143

The test-retest reliability and agreement between a fixed frame and belt-stabilised handheld dynamometer for isometric hip flexion and extension peak force measurement in recreational cyclists

Dion D’Mello 1, Benn Digweed 1,2, Tom Hughes 1,3,*
Editor: Sohel Ahmed4
PMCID: PMC13048430  PMID: 41931523

Abstract

Introduction

Cycling performance is influenced by hip flexor and extensor muscle strength. While belt stabilised handheld dynamometers (B-HHD) are valid for measuring isometric hip muscle strength, fixed frame dynamometers are becoming popular, offering potentially better stability and reliability. However, the reliability of both devices has not been examined in cyclists. This study evaluated the test-retest reliability and agreement between a B-HHD (MicroFET2, Hoggan Scientific) and a fixed-frame dynamometer (ForceFrame (FF) Max, Vald Performance) for hip flexion and extension peak force measurement in cyclists.

Methods

A test-retest design was used. Twenty-five recreational cyclists (age ± SD: 36.64 (±12.34) years; 22 males) were tested twice, approximately 72 hours apart. Three unilateral maximal voluntary isometric contractions (MVIC) of the hip flexors and extensors of each limb were performed, using the B-HHD and FF in a random order. Within and between session reliability was determined using intraclass correlation coefficients (ICCs) 3.1 & 3.k. Standard error of measurements (SEM) and minimal detectable changes (MDC) were calculated. Agreement was assessed using 95% limits of agreement (LOA).

Results

For hip flexion, within and between session reliability was good to excellent, and SEMs were similar (B-HHD ICCs = 0.77–0.93, SEMs = 14.25–22.71N (7.19–10.38%); FF ICCs = 0.77–0.95, SEMs = 7.80N–18.98N (3.47%−8.54%)). FF MDCs were lower within-session (21.61–39.48N (9.60–17.97%)) than B-HHD MDCs (39.50–62.95N (19.94–28.78%)), but similar between-sessions (FF MDCs = 41.25–52.61N (19.42–23.66%); B-HHD MDCs = 41.21N–48.95N (18.53–23.77%)).

For hip extension, both devices demonstrated good to excellent reliability and SEMs were similar (B-HHD ICCs = 0.90–0.95, SEMs = 15.77–21.53N (7.38–9.96%); FF ICCs = 0.85–0.95, SEMs = 19.21–29.05N (7.82–11.78%) within and between sessions). All LOA exceeded a 20N acceptability threshold.

Conclusion

Both devices are reliable in recreational cyclists, but large MDCs suggest that caution is needed when interpreting repeated measurements. Due to poor agreement, the devices are not interchangeable so should be considered device specific. In practice, our preliminary results suggest FF data cannot be compared with B-HHD data and vice versa, so the same device should be used for repeated measurements in this population.

Introduction

Cycling places physiological stress on the metabolic, cardiovascular and musculoskeletal systems, which can improve cardiovascular fitness [1], cognitive function and overall wellbeing [2]. It is associated with a reduced risk of cardiovascular disease (CVD) and related risk factors (including obesity, hypertension and elevated trigylcerides) [3]. Cycling is linked to a 17% reduction in CVD mortality [3] and a 20% reduction in all-cause mortality [1], making it a highly recommended activity for public health improvement [4]. Additionally, cycling has become increasingly popular in the United States (US) [5,6] and other European countries [7,8] for transportation, recreational [5] and competitive purposes [6].

Approximately 85% of cycling power is generated during the pedal downstroke [9] by the hip extensor, knee extensor, and ankle plantarflexor muscles [10]. During pedal upstroke, power is generated by the hip and knee flexors, with biarticular muscles maintaining joint stability [11]. To enhance muscular strength and cycling performance, recreational and competitive cyclists commonly combine resistance training with cycling endurance training [12]. Indeed, resistance training has been shown to improve peak power output in competitive cyclists [13,14], cycling economy and VO2 Max in moderately trained cyclists [15,16] and pedalling cadence efficiency in recreational athletes [14,16,17]. However, these latter benefits are less clear in highly trained cyclists [12,14–16].

Given the importance of hip strength in cycling performance and the risk of injury associated with cycling participation [18], regular muscle strength assessments may provide valuable data for cyclists, coaches and health professionals. Indeed, regular physical testing can be useful for performance tracking, training evaluation and planning [19–21], setting rehabilitation targets [19] and assessing treatment effectiveness following injury [22].

Isokinetic dynamometers (IKD) are considered the gold standard for muscle strength assessment due to their reliability and ability to measure various strength components (including peak force, work, power, endurance and force curves) [22]. However, IKDs are expensive, non-portable and require expert training, limiting their use to clinical [23,24] and research settings. In contrast, portable and simple hand-held dynamometers (HHDs) (such as the MicroFET2, Hoggan Scientific, Salt Lake City, USA) are commonly used to measure isometric strength, due to their cost-effectiveness, mass-testing capabilities [25], accessibility, and ease of use without specialist training [26]. HHDs can quantify maximal isometric voluntary contractions (MVICs), which are valid assessments of maximal strength [21].

HHDs show acceptable intra and inter-rater reliability for measuring isometric hip flexion and extension MVICs (weighted correlation coefficient (WCC) ranges: 0.79–0.91 and 0.81–0.87, respectively) [27]. However, measurements can be influenced by the examiner’s strength, potentially causing inter-rater bias [28], and the lack of device stabilisation can introduce errors [29]. External fixation methods, such as belt stabilisation, can eliminate examiner-provided resistance and improve test-retest reliability (WCC ranges = 0.91–0.93, ICC 2.1 = 0.91–0.95) [27,30]. Importantly, population characteristics influence device reliability [31,32], but to date, the reliability of external, belt-stabilised HHDs (B-HHDs) has not been investigated in cyclists, so it is unclear if these estimates apply to this population.

While belt stabilisation may improve reliability, newer fixed frame dynamometers (such as the Vald ForceFrame Max (Vald Performance, Brisbane, Australia)), may offer superior stabilisation because sensors are mounted directly to a frame and base. Fixed frame devices are also highly adjustable, which allows testing of most muscle groups. A different iteration of the ForceFrame (i.e., ForceFrame Fold) has shown good to excellent test-retest reliability for hip adduction (ICC2.1 = 0.81–0.90) and moderate to excellent test-retest reliability for hip abduction MVICs (ICC 2.1 = 0.72–0.92) in field sport athletes [33]. However, to the best of our knowledge, no previous studies have investigated the reliability of any ForceFrame version for measuring hip flexion and extension MVICs in any population.

Given the growing preference to replace HHDs with fixed frame devices, [34] it is essential to determine whether measurements from both devices can be interchanged.a Establishing this would help ensure consistent and accurate data collection in settings where both devices are used, where longitudinal B‑HHD data already exist and a fixed‑frame device is being introduced, or when practitioners are deciding which measurement method to use.

The aim of this study was to evaluate the test-retest reliability and agreement between a belt-stabilised MicroFET2 HHD and the ForceFrame Max, for measurement of hip flexion and extension MVIC peak force in recreational cyclists.

Materials and methods

This study was reported according to The Guidelines for Reporting of Reliability and Agreement Studies [32] and the Consensus-based Standards for the Selection of health Measurement INstruments (COSMIN) Reporting Guideline for Studies on Measurement Properties of Patient Reported Outcomes Measures [35]. The methodology has been detailed a priori, in a protocol available on Open Science Framework [36].

Study design and setting

Because both devices were stabilised, examiner effects were considered negligible; therefore, a test–retest design was employed. Data were collected between 04/03/2024 and 22/03/2024 in a laboratory at Manchester Metropolitan University. The examiner was a Physiotherapy MSc student [36], with 3 years of experience using HHDs but no prior experience with the ForceFrame Max. Ten hours of pilot testing was completed for protocol refinement and familiarisation.

Participants

Recreational and competitive cyclists were recruited using purposive sampling. Posters were placed in cycle shelters at Manchester Metropolitan University and distributed to three cycling clubs in the Greater Manchester area. Potential participants voluntarily contacted the lead researcher for eligibility confirmation and study details.

Participants were included if they were aged >18 years and completed > 150 minutes cycling per week at ‘vigorous’ intensity (> 10 miles per hour as defined by the Centre for Disease Control and Prevention [37]).

Participants were excluded if they: had an active neurological, cardiovascular or respiratory disease; were pregnant; had any lacerations, abrasions, or contusions at the distal femur affecting the area of HHD and FF load cell sensor application during testing; reported any musculoskeletal injury or orthopaedic surgery to the lumbar spine, pelvis or lower limbs or suffered any systemic illness within 3 months of the first assessment day.

Ethical approval was granted by the Faculty of Health and Education Research Ethics and Governance Committee at Manchester Metropolitan University (no.59674) and the study was conducted in accordance with the 2013 Declaration of Helsinki. All participants provided written informed consent prior to participation.

Sample size

Sample size was calculated using the confidence interval (CI) width procedure of the ICC & SEM Power Sample Size Decision Assistant (https://iriseekhout.shinyapps.io/ICCpower) [38]. Previously reported intraclass correlation coefficents (ICCs) for hip flexion and extension strength measurements were 0.91 and 0.90 for HHDs with external stabilisation [27] and 0.77–0.86 for the Groinbar (Vald Performance, Brisbane, Australia) [23], a similar fixed frame device to the Forceframe Max. For a two-way mixed effects ICC model that is appropriate for a test-retest design [39] as used in this study, our calculation assumed a conservative expected correlation of 0.80, a moderate variance of 10 for between-participant test scores with no expected systematic differences, and aimed for a target CI width of 0.30. Our calculation indicated that recruitment of 25 participants would enable ICC estimation with CI precision of 0.26 for within-session analyses (three trials per testing session), and 0.33 for between-session analyses (mean of three trials compared over two testing sessions) (see S1 Appendix for full calculation).

Instrumentation and measurement parameters

For all participants, self-reported weekly cycling activity level (minutes) and the usual mode of cycling participation were collected. Baseline measurements of standing height (centimetres) and body mass (kilograms) were recorded on the first testing day using the Leicester Height Measure (Seca Ltd., Birmingham, UK) and the Personal Floor Scale MPE 250K100HM (Kern & Sohn, Germany).

A belt-stabilised Micro-FET2 HHD (B-HHD) and ForceFrame Max Strength Testing System (FF) were used to assess hip flexion and hip extension peak force during MVICs, recorded directly into a laptop computer. FF measurements were transferred to an offline version of the Vald Uploader Manager (Vald Performance, Brisbane, Australia) using a wired connection. B-HHD measurements were manually entered into an Excel (Microsoft, Washington, USA) spreadsheet by the examiner. The B-HHD was calibrated at the start of each testing day. The FF was zeroed between each test. Peak force from all MVICs were recorded in newtons (N) and retained on their continuous scale for analyses.

Experimental procedure

All data were collected by the primary researcher, who was unblinded. Before each testing session, participants completed a standardised 5-minute warm-up on a stationary bicycle at a self-determined moderate intensity. Across both testing days, participants completed unliateral hip strength assessments with the B-HHD and FF, for both limbs. To eliminate order effects, tests were randomised by muscle group, devices and limb, using the RAND function in Excel, conducted by an independent person.

For B-HHD hip flexion, participants were seated upright on an examination table with their arms crossed against their chest. For FF hip flexion, due to device constraints, testing was conducted with participants in supine with arms crossed against their chest. The examiner used a goniometer to ensure the hip was positioned to 90° flexion for both devices. The sensor for each device was positioned along the distal femur, 1 inch superior to the patellar base.

For B-HHD and FF extension, participants lay prone with their hands under their forehead. For both devices, the examiner used a goniometer to ensure the knee of the tested limb was flexed to 90°. The sensor of each device was positioned along the distal femur, 1 inch superior to the popliteal crease. All testing positions are presented in S2 Appendix. The individuals pictured in S2 Appendix have provided written informed consent (as outlined in PLOS consent form) to publish their image.

For the FF tests, the crossbar height was reproduced on the second testing day. For all B-HHD tests, the fixation belt was secured to the dynamometer and examination table. The examiner manually stabilised the B-HHD to eliminate slipping but did not exert any downward pressure on the sensor.

For each muscle group, device and limb, participants completed three practice trials at 50%, 70% and 90% of their self-perceived maximal efforts. Following these practice attempts, participants performed three 5-second MVICs. For each trial, the examiner provided a standardised instruction of “Begin now – 3, 2, 1 - GO - 1, 2, 3, 4, 5 - relax”. A 10-second rest period was given between trials and 2-minute rest periods were provided upon test completion to minimise fatigue and allow for repositioning.

Trials were considered invalid and repeated if: participants altered their test position; the B-HHD slipped; an error was detected on the VALD interface. Participants were withdrawn if they experienced pain in their lower limbs or lumbar spine during testing. Upon completion, data collection was repeated 72 hours later, or as close as possible to 72 hours if participants could not attend at that time.

Statistical analyses

Descriptive statistics (mean ± standard deviation (SD)) were calculated for height, body mass, cycling volume and age. Proportions were calculated for biological sex and cycling modes. The distribution of raw data was visually inspected using quantile quantile (QQ) plots.

For within-session analyses, peak force (N) was compared across all three trials for each device, muscle group and limb on each testing day. For between-session analyses, the mean of peak force measurements was calculated using all three trials for each participant according to device, muscle group, limb and testing day. Mean values were then compared across testing days.

Two-way mixed effects ICC models are required for test-retest designs [39], so within session ICCs3.1 and between session ICCs3.k were calculated with corresponding 95% CIs. ICCs were interpreted as poor < 0.40; fair = 0.40–0.70; good = 0.70–0.90; excellent > 0.90 [40]. Standard error of measurements (SEM) were calculated using the formula SEM=SD1−ICC. After ICC model fitting, all residuals were also inspected for normality using histograms. Minimal detectable changes (MDC) were calculated using the formula: MDC=SEM×1.96×2 [41]. SEM and MDC values were reported in newtons (N) and as percentages of mean values.

To determine within-session agreement, the distributions of between device differences were assessed using QQ plots according to muscle group tested, limb and testing day. Bland-Altman plots with 95% limits of agreement (LOA) were constructed [42]. Previously, the MicroFET2 HHD has been shown to have concurrent validity with a similar fixed frame dynamometer system (KangaTech KT360, KangaTech, Melbourne, Australia), with a reported absolute mean difference of 7.74N (95%CI = 1.86–16.40N) [43]. Therefore, agreement was considered clinically acceptable if the 95%LOA were within 20N. All statistical analyses were initially conducted by DD using SPSS 29 (IBM, New York, USA), and repeated by TH using STATA 18 (StataCorp LLC, Texas, USA) for accuracy.

Results

Study Participation and missing data

Of twenty-six participants recruited, one withdrew due to pain during testing and was excluded from the analysis. In the remaining dataset, there were no instances of missing data. The remaining participants included 22 males and 3 females with the following characteristics (mean ± SD): age 36.64 (±12.34) years; height 180.36 (±7.76) cm; mass 80.25 (± 9.86) kg; cycling activity 294 (± 183.24) minutes per week. Ten participants (40%) primarily cycled for travel, 10 (40%) were recreational road cyclists, 3 (12%) were mountain bikers and 2 (8%) used a stationary cycle at the gym.

Within-session Reliability

For the within-session analyses, descriptive statistics are presented in Table 1. Peak force data and model residuals were approximately normally distributed across devices, limbs and testing days (S3 Appendix). ICCs, 95% CIs, SEMs and MDCs for each limb, device and testing day are presented in Table 2.

Table 1. Within-session descriptive statistics for peak force measurements, per device and testing day.

Within- session
Descriptive Statistics
Session 1 Session 2
Left limb Right limb Left Limb Right limb
Measure System Mean SD Mean SD Mean SD Mean SD
Hip Flexion B-HHD 198.12 47.13 218.75 61.96 213.81 36.24 225.96 53.27
FF 206.33 39.09 219.67 45.92 218.47 35.10 225.08 35.17
Hip Extension B-HHD 206.19 69.35 214.86 66.79 213.67 70.67 217.28 73.03
FF 251.57 85.00 243.87 70.70 241.71 66.23 247.56 76.15

Key: B-HHD = belt stabilised handheld dynamometer; FF = ForceFrame; SD = standard deviation. Please note that all measurements are in newtons (N).

Table 2. Within-session ICCs3.1, 95%CIs, SEM and MDC estimates for both devices and limbs, according to testing day.

Within- session Reliability Session 1 Session 2
Left limb Right limb Left Limb Right limb
Muscle group System ICC 95% CI SEM
(%)
MDC
(%)
ICC 95% CI SEM
(%)
MDC
(%)
ICC 95% CI SEM
(%)
MDC
(%)
ICC 95% CI SEM
(%)
MDC
(%)
Hip Flexion B-HHD 0.91 0.83–0.96 14.25
(7.19)
39.50
(19.94)
0.87 0.76–0.93 22.71
(10.38)
62.95
(28.78)
0.77 0.60–0.88 17.55
(8.21)
48.65
(22.75)
0.84 0.71–0.92 21.51
(9.52)
59.63
(26.39)
FF 0.94 0.88- 0.97 9.77
(4.74)
27.07
(13.12)
0.90 0.82–0.95 14.24
(6.48)
39.48
(17.97)
0.89 0.79–0.95 11.75
(5.38)
32.58
(14.91)
0.95 0.91–0.98 7.80
(3.47)
21.61
(9.60)
Hip Extension B-HHD 0.93 0.87–0.97 18.66
(9.05)
51.73
(25.09)
0.90 0.82–0.95 20.98
(9.76)
58.16
(27.07)
0.95 0.91–0.98 15.77
(7.38)
43.71
(20.46)
0.95 0.90–0.97 16.86
(7.78)
46.74
(21.51)
FF 0.95 0.90–0.98 19.35
(7.69)
53.63
(21.32)
0.93 0.87–0.97 18.71
(7.67)
51.88
(21.27)
0.87 0.76–0.93 24.22
(10.02)
67.12
(27.77)
0.93 0.87–0.97 19.86
(8.02)
55.05
(22.23)

Note: SEM and MDC measures are presented in newtons (N), whilst (%) values present the estimates as a proportion relative to mean values. Key: B-HHD = belt stabilised handheld dynamometer; CI = confidence interval; FF = ForceFrame; ICC – intraclass correlation coefficient; MDC = minimal detectable change; SEM = standard error of measurement.

For hip flexion, both devices generally showed comparable good to excellent reliability and similar SEMs. Across both days and limbs, FF ICCs3.1 ranged between 0.89–0.95. B-HHD reliability of the left limb on day 2 was slightly lower (ICC3.1 = 0.77), but all other values were similar to the FF (ICC3.1= 0.84–0.91). FF SEMs were between 7.80–14.24N (or 3.47–6.48%). Similarly, B-HHD SEMs were between 14.25–22.71N (or 7.19–10.38%). However, FF MDCs were consistently lower (21.61–39.48N (9.60–17.97%)) than B-HHD MDCs (39.50–62.95N (19.94–28.78%)).

For hip extension, both devices showed good to excellent reliability with similar SEM and MDC estimates. Across both days and limbs, FF ICCs3.1 were 0.87–0.95 and B-HHD ICCs3.1 were 0.90–0.95. FF SEMs were between 18.71–24.22N (7.67–10.02%), and B-HHD SEMs were 15.77–20.98N (7.38–9.76%). FF MDCs were between 51.88–67.12N (21.27–27.77%) and B-HHD MDCs were 43.71–58.16N (20.46–27.07%).

Between-session reliability

Descriptive statistics, ICCs3.k, 95% CIs, SEMs and MDCs are presented in Table 3.

Table 3. Between-session descriptive statistics, ICCs, 95%CIs, SEM and MDC estimates for both devices, according to limb.

Between- session Reliability Left limb Right limb
Measure System Mean SD ICC 95% CI SEM
(%)
MDC
(%)
Mean SD ICC 95% CI SEM
(%)
MDC
(%)
Hip Flexion B-HHD 205.96 40.80 0.81 0.53–0.92 17.66
(8.57)
48.95
(23.77)
222.35 55.08 0.93 0.84 – 0.97 14.87
(6.69)
41.21
(18.53)
FF 212.40 36.68 0.84 0.61–0.93 14.88
(7.01)
41.25
(19.42)
222.37 40.00 0.77 0.49–0.90 18.98
(8.54)
52.61
(23.66)
Hip Extension B-HHD 209.93 68.85 0.92 0.82–0.97 19.48
(9.28)
54.01
(25.73)
216.07 68.41 0.90 0.78–0.96 21.53
(9.96)
59.67
(27.62)
FF 246.64 74.39 0.85 0.66–0.93 29.05
(11.78)
80.51
(32.64)
245.71 71.97 0.93 0.84 – 0.97 19.21
(7.82)
53.26
(21.68)

Note: SEM and MDC measures are presented in newtons (N), whilst (%) values present the estimates as a proportion relative to mean values. Key: B-HHD = belt stabilised handheld dynamometer; CI = confidence interval; FF = ForceFrame; ICC – intraclass correlation coefficient; MDC = minimal detetcable change; SEM = standard error of measurement.

For hip flexion, FF reliability was consistently good (ICC3.k. range = 0.77–0.84). B-HHD reliability was good on the left limb (ICC3.k= 0.81) and excellent on the right (ICC3.k = 0.93). For both devices, SEMs and MDCs were similar (SEMs: FF = 14.88–18.98N (7.01–8.54%), B-HHD = 14.87–17.66N (6.69–8.57%); MDCs: FF = 41.25–52.61N (19.42–23.66%), B-HHD 41.21N–48.95N (18.53–23.77%)).

For hip extension, both devices had good to excellent reliability across limbs (FF ICC3.k = 0.85–0.93, B-HHD ICC3.k = 0.90–0.92). SEMs and MDCs were also comparable (SEM: FF = 19.21–29.05N (7.82–11.78%), B-HHD = 19.48–21.53N (9.28–9.96%); MDCs: FF = 53.26–80.51N (21.68–32.64%), B-HHD 54.01–59.67N (25.73–27.62%)).

Between- device agreement

The distribution of between-device differences was approximately normal across limbs, testing days and muscle groups (S4 Appendix). Bland and Altman plots are presented for hip flexion (Fig 1) and extension (Fig 2). All estimates for mean differences and LOA are presented in S5 Appendix.

Fig 1. Within-session agreement between devices for hip flexion peak force measurement (both limbs and testing days).

Fig 1

Note: The Y axis (difference between devices) at 0 indicates perfect agreement; positive values show the ForceFrame recorded greater values, whereas negative values show the Belt-stabilised handheld dynamometer recorded greater values. Thick black lines correspond to 95% limits of agreement, dashed black lines represent observed mean agreement. Key: B-HHD = Belt-stabilised handheld dynamometer; diff = difference, FF = ForceFrame; LOA = limits of agreement; n = newtons.

Fig 2. Within-session agreement between devices for hip extension peak force measurement (both limbs and testing days).

Fig 2

Note: The Y axis (difference between devices) at 0 indicates perfect agreement; positive values show the Forceframe recorded greater values, whereas negative values show the Belt-stabilised handheld dynamometer recorded greater values. Thick black lines correspond to 95% limits of agreement, dashed black lines represent observed mean agreement. Key: B-HHD = Belt-stabilised handheld dynamometer; diff = difference, FF = ForceFrame; LOA = limits of agreement; n = newtons.

For hip flexion, mean agreement ranged from −0.88 to 8.22 N across limbs and testing days. For hip extension, the FF consistently produced higher peak force measures (mean agreement range = 28.04 to 45.38 N) across both limbs and days. All 95% LOAs were very wide, exceeding the 20N acceptable threshold, ranging from 126.98N (Fig 1, plot C) to 289.09N (Fig 2, plot D).

Discussion

This is the first study to evaluate test-retest reliability and agreement between a belt-stabilised, handheld MicroFET2 dynamometer and the Forceframe Max system for measuring peak hip flexion and extension force in cyclists, offering novel data. Although both devices initially appear reliable, the observed MDC and agreement values carry potentially important practical and clinical implications.

Reliability and measurement error

For hip flexion, both devices demonstrated good to excellent within and between-session reliability, and measurement error. Specifically, B-HHD ICCs ranged between 0.77–0.93, with SEMs of 14.25N–22.71N (7.19%−10.38%). These values are generally comparable to a recent meta-analysis of portable, externally stabilised dynamometers for seated hip flexion MVIC assessments, which reported intra-rater WCCs of 0.91 (95%CI = 0.84–0.99) and average SEMs of 15.00N. This similarity was expected, as most devices included in the meta-analysis used comparable B-HHDs, seated testing positions and protocols to those used in our study.

We also found that between-session B-HHD estimates (ICC 3.k = 0.81–0.93) were comparable to those recently reported for peak torque by McNabb et al (ICC2.1 = 0.91) [30]. However, our B-HHD SEMs (6.69%−8.57%) indicated greater precision than the 14% reported by McNabb et al [30]. While both studies used similar protocols and equipment, this subtle difference may be explained by the distinct constructs measured between studies. While related, peak force (used in our study) is the maximal linear force produced during a task, whereas peak torque (used by McNabb et al [30]) is the maximal rotational force produced during a task [44]. Population differences may have also contributed, as we studied recreational cyclists, rather than a general cohort of healthy adults.

As this is the first study to evaluate the Forceframe Max for hip flexion, direct comparisons to other studies are not possible. A previous meta-analysis reported that externally stabilised dynamometers in supine (as tested in our study) had excellent intra-tester reliability for (WCC = 0.93, 95%CI = 0.89–0.98; average SEMs = 35.50N) [27]. We found that within session FF reliability was consistent with these findings (ICC3.1 = 0.89–0.95), but between-session reliability was slightly lower (ICCs3.k = 0.77–0.84). However, the FF offered greater precision both within and between-sessions (SEM = 7.80 – 18.98N (3.47–8.54%)). This may reflect differences in the devices included in the meta-analysis, which consisted of various B-HHDs and other dynamometers that may not be directly comparable with the FF.

Our results closely aligned with those reported for hip adduction (test-retest ICC2.1 = 0.81–0.90; SEM = 6.4–8.6%)) and adduction (test-retest ICC 2.1 = 0.72–0.92 SEM = 4.3–11.9%) peak force measurment, using a different iteration of the FF in field sport athletes [33]. These findings tentatively suggest that different FF models may perform consistently across populations and hip muscle groups, though further studies are needed to confirm this.

For hip extension, both devices demonstrated good to excellent reliability and similar measurement error (B-HHD ICCs = 0.90–0.95, SEMs = 15.77−21.53N (or 7.38–9.96%); FF ICCs = 0.85–0.95, SEMs = 19.21−29.05N (7.82–11.78%) within and between sessions). These were comparable with values previously reported in a previous meta-analysis of externally stabilised dynamometers used in prone positions (WCC = 0.90; 95%CI = 0.66–0.99; average SEM = 16.90N) [27]. Similarly to hip flexion, we also found B-HHD reliability was consistent with values reported by McNabb et al. [30] for peak torque (ICC2.1 = 0.95; SEM = 12.00%), although SEM were marginally less in our study [30].

As before, the FF cannot be compared with previous hip extension data, though our observed values were similar to those previously discussed, from a different ForceFrame iteration for hip abduction and abduction peak force [33].

Collectively, these findings suggest that while both devices in our study are reliable for hip extension MVIC peak force measurement, the close consistency with other dynamometers, muscle groups and measurement parameters may be a function of the stable prone test position for hip extension, but this should be also be confirmed in future studies.

Minimal detectable change and clinical implications

Our observed MDC values for both devices suggest potential practical limitations for both devices. For hip flexion, the FF demonstrated consistently lower within-session MDCs (FF = 21.61N–39.48N (9.6–18.00%); B-HHD = 39.50-62.98N (20.0–28.8%)). However, between-session MDCs were similar (MDC range = 41.21–52.61N) and generally consistent with those reported for all externally stabilised portable dynamometers (MDC = 46.7N–49.3N) [27], but slightly less than 32% observed for peak torque measurement by McNabb et al [30] using a B-HHD. For hip extension, the MDCs observed for both devices (MDCs = 43.71–67.12N (20.50–27.80%) were also broadly comparable with these previous reports [27,30].

MDC values are crucial for distinguishing true performance changes from measurement error [41]. Considering that strength changes of less than 15% are clinically and practically important [26], all within-session B-HHD MDCs, and three quarters of within session FF MDCs were greater than 15% of mean values. Additionally, all between-session MDCs were greater than 15% of mean values for both devices (range = 18.50% to 32.60%). This raises some concerns about the sensitivity of both devices to detect subtle but meaningful changes in performance both within a session (e.g., following physiotherapy interventions such as manual therapy or proprioceptive taping) or over time as a response to rehabilitation or training.

For illustration, isometric training may result in strength improvements of 4.34% per week [45], and 21.5% after 6–12 weeks [46] in healthy adults. Dynamic resistance training can result in strength improvements of 1.77% [47] to 5.20% [48] per week, increasing to between 27.00% and 32.00% after 6 weeks [46,47,49].

Because these weekly adaptations are considerably less than all observed MDC values, it is unlikely that either device could detect true peak force changes if measured on a week-to-week basis. Furthermore, the similarity between six-week adaptations and between-session MDC estimates suggests that that any longer term (i.e., > 6 week) changes should also be cautiously interpreted as these may still be influenced by measurement error.

Between system agreement

Despite established concurrent validity between the MicroFET2 and Kangatech KT360 [43] (a similar device to the FF), the poor agreement between for hip flexion and extension was surprising, particularly given how far the 95% LOA were exceeded. Possible contributing factors include differences in device resolution (FF = 1.00N; MicroFET B-HHD = 0.44N) [50,51] and the potentially greater stability offered by the FF’s rigid aluminium frame and base, compared to the B-HHD’s fabric belt. Specifically for hip flexion, differences in testing positions may have introduced additional variation. Theoretically, the seated position used with the B-HHD may be less stable than the supine position used with the FF. However, this did not appear to systematically bias the measurements, as the mean agreement was low (range = −0.88–8.22 N). In contrast, there was consistent, larger bias for hip extension, with the FF recording higher peak force (28.04 to 45.38 N). Importantly, across all measurement scenarios presented in Figs 1 and 2, even adjusting values by the largest recorded mean difference (45.38N), the LOA would still exceed the 20N acceptability threshold, making this an unhelpful transformation.

Due to the observed poor agreement, hip flexion and extension peak force data cannot be interchanged between devices in this population. In practice, this means that measurements should be considered as device-specific; we cautiously recommend against comparing FF data with B-HHD data (and vice versa), and suggest the same device should be used for repeated measurements.

Limitations

Our study has some limitations. The marginal increase in peak force between days suggests a minor learning effect despite familiarisation. While no evidence of fatigue was observed, the validated Rating of Fatigue Scale [52], or other subjective wellbeing scores [53] could have quantified fatigue effects. The second test session was conducted approximately 72 hours after the first, but there was some variability around this due to participant’s external commitments, so could be more robustly controlled for in future studies.

Finally, since we used two-way mixed effects (ICC 3.1 and 3.k) models, our findings are not generalisable [38]. Future studies should replicate and confirm our data across different populations. This includes elite cylists, who are likely to utilise regular strength assessments.

Conclusion

This study found that that the MicroFET2 and Forceframe Max have good to excellent reliability and comparable precision for assessing hip flexor and extensor peak force in recreational cyclists. However, their MDC values mean that these devices have potentially limited clinical and practical usefulness in this population. The poor observed agreement means that interchangeability between devices cannot currently be recommended. Given the two-way mixed effects ICC models used, further studies are recommended in various populations to confirm these findings.

Supporting information

S1 Appendix. Sample size calculation.

(DOCX)

pone.0328143.s001.docx (240KB, docx)
S2 Appendix. Testing positions.

(DOCX)

S3 Appendix. Distribution of raw scores and residuals.

(DOCX)

pone.0328143.s003.docx (12.8MB, docx)
S4 Appendix. Distribution of between device differences.

(DOCX)

pone.0328143.s004.docx (3.2MB, docx)
S5 Appendix. Limits of agreement estimates.

(DOCX)

Acknowledgments

The authors would like to thank all staff within the Manchester Metropolitan University Sport team, British Cycling Clubs and Community Groups for their help and support with participant recruitment (without whom this study would not be possible). The authors also thank: Katie Flatters and Heather Stephens at British Cycling for support with study conception; Prof. Michael Callaghan at Manchester Metropolitan University for methodology advice; Dr. Jamie Sergeant at University of Manchester for statistical advice and Liam Parkinson from VALD Performance for providing the VALD ForceFrame Max.

Data Availability

An anonymised version of the dataset and Stata code is accessible via Manchester Metropolitan University’s data repository (E-space), and is freely available at https://doi.org/10.23634/MMU.00643463.

Funding Statement

Neither the authors nor Manchester Metropolitan University received funding for this work. No funding or incentives were provided by Vald Performance to conduct this work and the ForceFrame Max used in this study was loaned to Manchester Metropolitan University at no cost.

References

  • 1.Oja P, Titze S, Bauman A, de Geus B, Krenn P, Reger-Nash B, et al. Health benefits of cycling: a systematic review. Scand J Med Sci Sports. 2011;21(4):496–509. doi: 10.1111/j.1600-0838.2011.01299.x [DOI] [PubMed] [Google Scholar]
  • 2.Leyland L-A, Spencer B, Beale N, Jones T, van Reekum CM. The effect of cycling on cognitive function and well-being in older adults. PLoS One. 2019;14(2):e0211779. doi: 10.1371/journal.pone.0211779 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Nordengen S, Andersen LB, Solbraa AK, Riiser A. Cycling is associated with a lower incidence of cardiovascular diseases and death: Part 1 - systematic review of cohort studies with meta-analysis. Br J Sports Med. 2019;53(14):870–8. doi: 10.1136/bjsports-2018-099099 [DOI] [PubMed] [Google Scholar]
  • 4.National Institute of Health and Care Excellence (NICE). Walking and cycling: local measures to promote walking and cycling as forms of travel or recreation. 2012. https://www.nice.org.uk/guidance/ph41 [Google Scholar]
  • 5.Pucher J, Buehler R, Seinen M. Bicycling renaissance in North America? An update and re-appraisal of cycling trends and policies. Transportation Research Part A: Policy and Practice. 2011;45(6):451–75. doi: 10.1016/j.tra.2011.03.001 [DOI] [Google Scholar]
  • 6.Jancaitis G, Snyder Valier AR, Bay C. A descriptive and comparative analysis of injuries reported in USA Cycling-sanctioned competitive road cycling events. Inj Epidemiol. 2022;9(1):22. doi: 10.1186/s40621-022-00385-7 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Buehler R, Pucher J, Merom D, Bauman A. Active travel in Germany and the U.S. Contributions of daily walking and cycling to physical activity. Am J Prev Med. 2011;41(3):241–50. doi: 10.1016/j.amepre.2011.04.012 [DOI] [PubMed] [Google Scholar]
  • 8.Department of Transport. Road Traffic Estimates: Great Britain 2021. London: Department for Transport. 2021. [Google Scholar]
  • 9.Dorel S, Couturier A, Lacour J-R, Vandewalle H, Hautier C, Hug F. Force-velocity relationship in cycling revisited: benefit of two-dimensional pedal forces analysis. Med Sci Sports Exerc. 2010;42(6):1174–83. doi: 10.1249/MSS.0b013e3181c91f35 [DOI] [PubMed] [Google Scholar]
  • 10.Martin-Rodriguez S, Gonzalez-Henriquez JJ, Bautista IJ, Calbet JAL, Sanchis-Moysi J. Interplay of Muscle Architecture, Morphology, and Quality in Influencing Human Sprint Cycling Performance: A Systematic Review. Sports Med Open. 2024;10(1):81. doi: 10.1186/s40798-024-00752-2 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.So RC, Ng JK-F, Ng GY. Muscle recruitment pattern in cycling: a review. Physical Therapy in Sport. 2005;6(2):89–96. [Google Scholar]
  • 12.Yamamoto LM, Klau JF, Casa DJ, Kraemer WJ, Armstrong LE, Maresh CM. The effects of resistance training on road cycling performance among highly trained cyclists: a systematic review. J Strength Cond Res. 2010;24(2):560–6. doi: 10.1519/JSC.0b013e3181c86583 [DOI] [PubMed] [Google Scholar]
  • 13.Bastiaans JJ, van Diemen AB, Veneberg T, Jeukendrup AE. The effects of replacing a portion of endurance training by explosive strength training on performance in trained cyclists. Eur J Appl Physiol. 2001;86(1):79–84. doi: 10.1007/s004210100507 [DOI] [PubMed] [Google Scholar]
  • 14.Rønnestad BR, Hansen EA, Raastad T. Effect of heavy strength training on thigh muscle cross-sectional area, performance determinants, and performance in well-trained cyclists. Eur J Appl Physiol. 2010;108(5):965–75. doi: 10.1007/s00421-009-1307-z [DOI] [PubMed] [Google Scholar]
  • 15.Vikmoen O, Rønnestad BR. A Comparison of the Effect of Strength Training on Cycling Performance between Men and Women. J Funct Morphol Kinesiol. 2021;6(1):29. doi: 10.3390/jfmk6010029 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Rønnestad BR, Hansen J, Hollan I, Ellefsen S. Strength training improves performance and pedaling characteristics in elite cyclists. Scand J Med Sci Sports. 2015;25(1):e89-98. doi: 10.1111/sms.12257 [DOI] [PubMed] [Google Scholar]
  • 17.Hansen EA, Raastad T, Hallén J. Strength training reduces freely chosen pedal rate during submaximal cycling. Eur J Appl Physiol. 2007;101(4):419–26. doi: 10.1007/s00421-007-0515-7 [DOI] [PubMed] [Google Scholar]
  • 18.Clarsen B, Pluim BM, Moreno-Pérez V, Bigard X, Blauwet C, Del Coso J, et al. Methods for epidemiological studies in competitive cycling: an extension of the IOC consensus statement on methods for recording and reporting of epidemiological data on injury and illness in sport 2020. Br J Sports Med. 2021;55(22):1262–9. doi: 10.1136/bjsports-2020-103906 [DOI] [PubMed] [Google Scholar]
  • 19.Hughes T, Sergeant JC, van der Windt DA, Riley R, Callaghan MJ. Periodic Health Examination and Injury Prediction in Professional Football (Soccer): Theoretically, the Prognosis is Good. Sports Med. 2018;48(11):2443–8. doi: 10.1007/s40279-018-0928-y [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Jamnick NA, Pettitt RW, Granata C, Pyne DB, Bishop DJ. An Examination and Critique of Current Methods to Determine Exercise Intensity. Sports Med. 2020;50(10):1729–56. doi: 10.1007/s40279-020-01322-8 [DOI] [PubMed] [Google Scholar]
  • 21.Suchomel TJ, Nimphius S, Stone MH. The Importance of Muscular Strength in Athletic Performance. Sports Med. 2016;46(10):1419–49. doi: 10.1007/s40279-016-0486-0 [DOI] [PubMed] [Google Scholar]
  • 22.Stark T, Walker B, Phillips JK, Fejer R, Beck R. Hand-held dynamometry correlation with the gold standard isokinetic dynamometry: a systematic review. PM R. 2011;3(5):472–9. doi: 10.1016/j.pmrj.2010.10.025 [DOI] [PubMed] [Google Scholar]
  • 23.Desmyttere G, Gaudet S, Begon M. Test-retest reliability of a hip strength assessment system in varsity soccer players. Phys Ther Sport. 2019;37:138–43. doi: 10.1016/j.ptsp.2019.03.013 [DOI] [PubMed] [Google Scholar]
  • 24.Martins J, da Silva JR, da Silva MRB, Bevilaqua-Grossi D. Reliability and Validity of the Belt-Stabilized Handheld Dynamometer in Hip- and Knee-Strength Tests. J Athl Train. 2017;52(9):809–19. doi: 10.4085/1062-6050-52.6.04 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Jackson SM, Cheng MS, Smith AR Jr, Kolber MJ. Intrarater reliability of hand held dynamometry in measuring lower extremity isometric strength using a portable stabilization device. Musculoskelet Sci Pract. 2017;27:137–41. doi: 10.1016/j.math.2016.07.010 [DOI] [PubMed] [Google Scholar]
  • 26.Chamorro C, Armijo-Olivo S, De la Fuente C, Fuentes J, Javier Chirosa L. Absolute Reliability and Concurrent Validity of Hand Held Dynamometry and Isokinetic Dynamometry in the Hip, Knee and Ankle Joint: Systematic Review and Meta-analysis. Open Med (Wars). 2017;12:359–75. doi: 10.1515/med-2017-0052 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Waiteman MC, Garcia MC, Briani RV, Norte G, Glaviano NR, De Azevedo FM, et al. Can Clinicians Trust Objective Measures of Hip Muscle Strength From Portable Dynamometers? A Systematic Review With Meta-analysis and Evidence Gap Map of 107 Studies of Reliability and Criterion Validity Using the COSMIN Methodology. J Orthop Sports Phys Ther. 2023;53(11):655–72. doi: 10.2519/jospt.2023.12045 [DOI] [PubMed] [Google Scholar]
  • 28.Thorborg K, Bandholm T, Schick M, Jensen J, Hölmich P. Hip strength assessment using handheld dynamometry is subject to intertester bias when testers are of different sex and strength. Scand J Med Sci Sports. 2013;23(4):487–93. doi: 10.1111/j.1600-0838.2011.01405.x [DOI] [PubMed] [Google Scholar]
  • 29.Kolber MJ, Cleland JA. Strength testing using hand-held dynamometry. Physical Therapy Reviews. 2005;10(2):99–112. doi: 10.1179/108331905x55730 [DOI] [Google Scholar]
  • 30.McNabb K, Sánchez MB, Selfe J, Reeves ND, Callaghan M. Handheld dynamometry: Validity and reliability of measuring hip joint rate of torque development and peak torque. PLoS One. 2024;19(8):e0308956. doi: 10.1371/journal.pone.0308956 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Mokkink LB, Terwee CB, Patrick DL, Alonso J, Stratford PW, Knol DL, et al. The COSMIN checklist for assessing the methodological quality of studies on measurement properties of health status measurement instruments: an international Delphi study. Qual Life Res. 2010;19(4):539–49. doi: 10.1007/s11136-010-9606-8 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Kottner J, Audige L, Brorson S, Donner A, Gajewski BJ, Hróbjartsson A, et al. Guidelines for Reporting Reliability and Agreement Studies (GRRAS) were proposed. Int J Nurs Stud. 2011;48(6):661–71. doi: 10.1016/j.ijnurstu.2011.01.016 [DOI] [PubMed] [Google Scholar]
  • 33.O’ Connor C, McIntyre M, Delahunt E, Thorborg K. Reliability and validity of common hip adduction strength measures: The ForceFrame strength testing system versus the sphygmomanometer. Phys Ther Sport. 2023;59:162–7. doi: 10.1016/j.ptsp.2022.12.010 [DOI] [PubMed] [Google Scholar]
  • 34.Ransom M, Saunders S, Gallo T, Segal J, Jones D, Jones M, et al. Reliability of a portable fixed frame dynamometry system used to test lower limb strength in elite Australian Football League players. J Sci Med Sport. 2020;23(9):826–30. doi: 10.1016/j.jsams.2020.03.006 [DOI] [PubMed] [Google Scholar]
  • 35.Gagnier JJ, Lai J, Mokkink LB, Terwee CB. COSMIN reporting guideline for studies on measurement properties of patient-reported outcome measures. Qual Life Res. 2021;30(8):2197–218. doi: 10.1007/s11136-021-02822-4 [DOI] [PubMed] [Google Scholar]
  • 36.D’Mello D, Digweed B, Hughes T. Test-retest reliability and agreement of a belt-stabilized handheld dynamometer and the ForceFrame strength testing system in hip strength testing of recreational cyclists (protocol). 2024. 10.17605/OSF.IO/8HB7M [DOI]
  • 37.Brown W, Naughton G, Oldenburg B, Owen N, Wright MC. Promoting physical activity. Atlanta: Centers for Disease Control and Prevention. 2010. [Google Scholar]
  • 38.Mokkink LB, de Vet H, Diemeer S, Eekhout I. Sample size recommendations for studies on reliability and measurement error: an online application based on simulation studies. Health Serv Outcomes Res Method. 2022;23(3):241–65. doi: 10.1007/s10742-022-00293-9 [DOI] [Google Scholar]
  • 39.Koo TK, Li MY. A Guideline of Selecting and Reporting Intraclass Correlation Coefficients for Reliability Research. J Chiropr Med. 2016;15(2):155–63. doi: 10.1016/j.jcm.2016.02.012 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 40.Coppieters M, Stappaerts K, Janssens K, Jull G. Reliability of detecting “onset of pain” and “submaximal pain” during neural provocation testing of the upper quadrant. Physiother Res Int. 2002;7(3):146–56. doi: 10.1002/pri.251 [DOI] [PubMed] [Google Scholar]
  • 41.Weir JP. Quantifying test-retest reliability using the intraclass correlation coefficient and the SEM. J Strength Cond Res. 2005;19(1):231–40. doi: 10.1519/15184.1 [DOI] [PubMed] [Google Scholar]
  • 42.Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;1(8476):307–10. doi: 10.1016/s0140-6736(86)90837-8 [DOI] [PubMed] [Google Scholar]
  • 43.Dunne C, Callaway AJ, Thurston J, Williams JM. Validity, reliability, minimal detectable change, and methodological considerations for HHD and portable fixed frame isometric hip and groin strength testing: A comparison of unilateral and bilateral testing methods. Phys Ther Sport. 2022;57:46–52. doi: 10.1016/j.ptsp.2022.07.002 [DOI] [PubMed] [Google Scholar]
  • 44.Garcia MAC, Fonseca DS, Souza VH. Handheld dynamometers for muscle strength assessment: pitfalls, misconceptions, and facts. Braz J Phys Ther. 2021;25(3):231–2. doi: 10.1016/j.bjpt.2020.09.003 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Oranchuk DJ, Storey AG, Nelson AR, Cronin JB. Isometric training and long-term adaptations: Effects of muscle length, intensity, and intent: A systematic review. Scand J Med Sci Sports. 2019;29(4):484–503. doi: 10.1111/sms.13375 [DOI] [PubMed] [Google Scholar]
  • 46.Schoenfeld BJ, Grgic J, Ogborn D, Krieger JW. Strength and Hypertrophy Adaptations Between Low- vs. High-Load Resistance Training: A Systematic Review and Meta-analysis. J Strength Cond Res. 2017;31(12):3508–23. doi: 10.1519/JSC.0000000000002200 [DOI] [PubMed] [Google Scholar]
  • 47.Moesgaard L, Beck MM, Christiansen L, Aagaard P, Lundbye-Jensen J. Effects of Periodization on Strength and Muscle Hypertrophy in Volume-Equated Resistance Training Programs: A Systematic Review and Meta-analysis. Sports Med. 2022;52(7):1647–66. doi: 10.1007/s40279-021-01636-1 [DOI] [PubMed] [Google Scholar]
  • 48.Jung R, Gehlert S, Geisler S, Isenmann E, Eyre J, Zinner C. Muscle strength gains per week are higher in the lower-body than the upper-body in resistance training experienced healthy young women-A systematic review with meta-analysis. PLoS One. 2023;18(4):e0284216. doi: 10.1371/journal.pone.0284216 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 49.Hagstrom AD, Marshall PW, Halaki M, Hackett DA. The Effect of Resistance Training in Women on Dynamic Strength and Muscular Hypertrophy: A Systematic Review with Meta-analysis. Sports Med. 2020;50(6):1075–93. doi: 10.1007/s40279-019-01247-x [DOI] [PubMed] [Google Scholar]
  • 50.Hoggan Scientific. MicroFET2 Digital Handheld Dynamometer [Internet]. Salt Lake City (UT): Hoggan Scientific. 2023. https://hogganscientific.com/product/microfet2-muscle-tester-digital-handheld-dynamometer/
  • 51.VALD. ForceFrame Technical Specifications. 2023. https://support.vald.com/hc/en-au/articles/4998527361049-ForceFrame-Technical-Specifications
  • 52.Micklewright D, St Clair Gibson A, Gladwell V, Al Salman A. Development and Validity of the Rating-of-Fatigue Scale. Sports Med. 2017;47(11):2375–93. doi: 10.1007/s40279-017-0711-5 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 53.Saw AE, Main LC, Gastin PB. Monitoring the athlete training response: subjective self-reported measures trump commonly used objective measures: a systematic review. Br J Sports Med. 2016;50(5):281–91. doi: 10.1136/bjsports-2015-094758 [DOI] [PMC free article] [PubMed] [Google Scholar]

Decision Letter 0

Sohel Ahmed

11 Sep 2025

-->PONE-D-25-34937-->-->The test-retest reliability and agreement between a fixed frame and belt-stabilised handheld dynamometer for isometric hip flexion and extension peak force measurement in recreational cyclists-->-->PLOS ONE

Dear Dr.  Hughes,-->-->

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Oct 26 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:-->

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

-->If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols....

We look forward to receiving your revised manuscript.

Kind regards,

Sohel Ahmed, BPT, MPT, MDMR

Academic Editor

PLOS ONE

Journal Requirements:

When submitting your revision, we need you to address these additional requirements.

1.Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

2. Thank you for stating the following in the Competing Interests section:

“DDM was a student at Manchester Metropolitan University and this work was submitted as a dissertation project for the qualification of MSc Physiotherapy. He was employed at Manchester Movement Unit and British Cycling as a Sports Massage therapist during data collection. The project was conceived during his employment with British Cycling. BD was employed by Manchester Metropolitan University and the UK Sports Institute. TH is employed at Manchester Metropolitan University.”

Please confirm that this does not alter your adherence to all PLOS ONE policies on sharing data and materials, by including the following statement: "This does not alter our adherence to  PLOS ONE policies on sharing data and materials.” (as detailed online in our guide for authors http://journals.plos.org/plosone/s/competing-interests). If there are restrictions on sharing of data and/or materials, please state these. Please note that we cannot proceed with consideration of your article until this information has been declared.

Please include your updated Competing Interests statement in your cover letter; we will change the online submission form on your behalf.

3. Please include your full ethics statement in the ‘Methods’ section of your manuscript file. In your statement, please include the full name of the IRB or ethics committee who approved or waived your study, as well as whether or not you obtained informed written or verbal consent. If consent was waived for your study, please include this information in your statement as well.

4. If the reviewer comments include a recommendation to cite specific previously published works, please review and evaluate these publications to determine whether they are relevant and should be cited. There is no requirement to cite these works unless the editor has indicated otherwise.

5. Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

[Note: HTML markup is below. Please do not edit.]

Reviewer's Responses to Questions

-->Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. -->

Reviewer #1: No

Reviewer #2: Yes

**********

-->2. Has the statistical analysis been performed appropriately and rigorously? -->

Reviewer #1: Yes

Reviewer #2: Yes

**********

-->3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.-->requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.-->

Reviewer #1: Yes

Reviewer #2: Yes

**********

-->4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.-->

Reviewer #1: Yes

Reviewer #2: Yes

**********

-->5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)-->

Reviewer #1: Study question is clear, but several issues limit the strength of the contribution, particularly in methodology, clarity of presentation, and robustness of conclusions

The Introduction does not clearly highlight the specific gap in the literature that this study addresses.

What does this study add that was not previously known

No sample size/power calculation is provided.

which covariates were adjusted for, rationale for chosen tests, handling of missing data

Tables/figures could be improved for clarity: labels are sometimes unclear; some redundancy exists between text and figures

Conclusions sometimes overstate findings relative to the data presented

Some sentences are overly long and could be simplified.

Reviewer #2: 1) Abstract could better highlight the clinical implications of poor agreement (e.g., why interchangeability matters in practice).

2) Potential bias: hip flexion positions differed (seated vs supine). This introduces systematic variation that may partly explain poor agreement. Needs stronger acknowledgment

3)Assumption of normality is mentioned but no normality test reported—should be clarified.

**********

-->6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our Privacy Policy..-->..-->

Reviewer #1: Yes:Ahmed Ibrahim Al KharusiAhmed Ibrahim Al KharusiAhmed Ibrahim Al KharusiAhmed Ibrahim Al Kharusi

Reviewer #2: Yes:Esedullah AKARASEsedullah AKARASEsedullah AKARASEsedullah AKARAS

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at . PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.. Please note that Supporting Information files do not need this step.-->. Please note that Supporting Information files do not need this step.. Please note that Supporting Information files do not need this step.-->

PLoS One. 2026 Apr 3;21(4):e0328143. doi: 10.1371/journal.pone.0328143.r002

Author response to Decision Letter 1


21 Oct 2025

Tom Hughes

Department of Health Professions

Manchester Metropolitan University

Email: t.hughes@mmu.ac.uk

Dear Dr Ahmed

Re: Revisions required for manuscript entitled: The test-retest reliability and agreement between a fixed frame and belt-stabilised handheld dynamometer for isometric hip flexion and extension peak force measurement in recreational cyclists (PONE-D-25-34937)

We would like to take the opportunity to extend our thanks to you and the reviewers for taking the time to consider our manuscript for publication in PLOS ONE. We are encouraged by the favourable response and have taken time to consider the feedback provided. We have now addressed these issues and feel that the manuscript offers a stronger submission as a result.

Please find our replies (in bold type) to the reviewer’s comments (in italic type), which explain how they have been dealt with in the paper. All amended text in the manuscript has been highlighted in yellow. We have copied and pasted excerpts of the text in the replies below where appropriate, to identify the changes made. Please note that line numbers referenced in the response relate to the Revised Manuscript with Tracked Changes.

Editor Comments

1.Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

Thank you for highlighting this. We have now amended the title page and adjusted formatting precisely throughout the document to ensure the manuscript conforms to publishing guidelines.

2. Thank you for stating the following in the Competing Interests section:

“DDM was a student at Manchester Metropolitan University and this work was submitted as a dissertation project for the qualification of MSc Physiotherapy. He was employed at Manchester Movement Unit and British Cycling as a Sports Massage therapist during data collection. The project was conceived during his employment with British Cycling. BD was employed by Manchester Metropolitan University and the UK Sports Institute. TH is employed at Manchester Metropolitan University.”

Please confirm that this does not alter your adherence to all PLOS ONE policies on sharing data and materials, by including the following statement: "This does not alter our adherence to PLOS ONE policies on sharing data and materials.” (as detailed online in our guide for authors http://journals.plos.org/plosone/s/competing-interests). If there are restrictions on sharing of data and/or materials, please state these. Please note that we cannot proceed with consideration of your article until this information has been declared.

We apologise for the omission of this statement. As requested, we have included the statement in the ‘Competing Interests’ section (line 627) which now states:

“DDM was a student at Manchester Metropolitan University and this work was submitted as a dissertation project for the qualification of MSc Physiotherapy. He was employed at Manchester Movement Unit and British Cycling as a Sports Massage therapist during data collection. The was conceived during his employment with British Cycling. BD was employed by Manchester Metropolitan University and the UK Sports Institute. TH is employed at Manchester Metropolitan University. This does not alter our adherence to PLOS ONE policies on sharing data and materials.”

Please include your full ethics statement in the ‘Methods’ section of your manuscript file. In your statement, please include the full name of the IRB or ethics committee who approved or waived your study, as well as whether or not you obtained informed written or verbal consent. If consent was waived for your study, please include this information in your statement as well.

Thank you for your comment and we apologise if this was unclear in the submitted document. This was already in place in the ‘Methods’ section, under the subheading of ‘Participants’. For clarity, we have also added that the study was conducted in accordance with the Declaration of Helskini in 2013, as data collection preceded publication of the updated Declaration on 19 October 2024. This text now states (line 214):

“Ethical approval was granted by the Faculty of Health and Education Research Ethics and Governance Committee at Manchester Metropolitan University (no.59674) and conducted in accordance with the 2013 Declaration of Helsinki. All participants provided written informed consent prior to participation.”

If the reviewer comments include a recommendation to cite specific previously published works, please review and evaluate these publications to determine whether they are relevant and should be cited. There is no requirement to cite these works unless the editor has indicated otherwise.

The reviewers have not recommended any citations to be added, so no action is needed here.

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

All references included have been checked again for completeness on 8th October 2025. No papers included have a retracted status. In relation to addressing a comment from Reviewer 1, we have added in a new reference (see below)

When completing the data availability statement of the submission form, you indicated that you will make your data available on acceptance. We strongly recommend all authors decide on a data sharing plan before acceptance, as the process can be lengthy and hold up publication timelines. Please note that, though access restrictions are acceptable now, your entire data will need to be made freely accessible if your manuscript is accepted for publication. This policy applies to all data except where public deposition would breach compliance with the protocol approved by your research ethics board. If you are unable to adhere to our open data policy, please kindly revise your statement to explain your reasoning and we will seek the editor's input on an exemption. Please be assured that, once you have provided your new statement, the assessment of your exemption will not hold up the peer review process.

Apologies for this; our data sharing plan has already been agreed, hence the reason why we have included our raw data and code; this has now been amended on the submission form.

Reviewer 1 Comments

Study question is clear, but several issues limit the strength of the contribution, particularly in methodology, clarity of presentation, and robustness of conclusions

The Introduction does not clearly highlight the specific gap in the literature that this study addresses.

What does this study add that was not previously known?

Thank you for taking the time and effort to review our paper, and we appreciate your feedback.

In relation to your point made above, throughout the introduction, we have explicitly outlined strong reasons for why this study is needed.

For example, on line 135, we highlight that because hip strength influences cycling performance, strength evaluation is important:

“Given the importance of hip strength in cycling performance and the risk of injury associated with cycling participation (18), regular muscle strength assessments may provide valuable data for cyclists, coaches and health professionals. Indeed, regular physical testing can be useful for performance tracking, training evaluation and planning (19-21), setting rehabilitation targets (19) and assessing treatment effectiveness following injury (22).”

On line 159, to justify investigating the use of belt stabilised handheld dynamometers (B-HHDs) in cyclists we explicitly state that:

“Importantly, population characteristics influence device reliability (31, 32), but to date, the reliability of external, belt-stabilised HHDs (B-HHDs) has not been investigated in cyclists, so it is unclear if these estimates apply to this population.”

We also justify the need to investigate fixed frame ForceFrame (FF) devices in cyclists (line 164):

“While belt stabilisation may improve reliability, newer fixed frame dynamometers (such as the Vald ForceFrame (herein termed FF) Max (Vald Performance, Brisbane, Australia)), may offer superior stabilisation because sensors are mounted directly to a frame and base. Fixed frame devices are also highly adjustable, which allows testing of most muscle groups. A different iteration of the FF (i.e. ForceFrame Fold) has shown good to excellent test-retest reliability for hip adduction (ICC2.1= 0.81-0.90) and moderate to excellent for hip abduction MVICs (ICC 2.1= 0.72-0.92) in field sport athletes (33). However, to the best of our knowledge, no previous studies have investigated the reliability of any FF version for measuring hip flexion and extension MVICs in any population.”

Finally, given the increased use of fixed frame devices to replace traditionally used B-HHDs in practice, we specifically state that (line 175):

“Given the growing preference to replace HHDs with fixed frame devices, (34) it is essential to determine whether measurements from both devices can be interchanged. This may help to ensure consistent and accurate data in settings where both devices are available, where longitudinal data have already been collected by B-HHDs, or when practitioners are deciding whether to use one method over another.

The aim of this study was to evaluate the test-retest reliability and agreement between a belt-stabilised MicroFET2 HHD and the ForceFrame Max, for measurement of hip flexion and extension MVIC peak force in recreational cyclists.”

Consequently, we feel that we have strongly justified and reasoned why this study is needed, and why its novelty will contribute to the existing evidence base. We therefore feel no change is necessary here.

No sample size/power calculation is provided.

Thank you for your comment. However, we have already specifically provided a sample size calculation (S1 Appendix), and there is a dedicated explanation of sample size in the Methods section on line 219, which states:

“Sample size was calculated using the confidence interval (CI) width procedure of the ICC & SEM Power Sample Size Decision Assistant (https://iriseekhout.shinyapps.io/ICCpower) (38). Previously reported intraclass correlation coefficents (ICCs) for hip flexion and extension strength measurements were 0.91 and 0.90 for HHDs with external stabilisation (27) and 0.77- 0.86 for the Groinbar, (Vald Performance, Brisbane, Australia) (23) a similar fixed frame device to the Forceframe Max used in this study. For a two-way mixed effects ICC model needed for a test-retest design, our calculation assumed a conservative expected correlation of 0.80, a moderate variance of 10 for between-participant test scores with no expected systematic differences, and aimed for a target CI width of 0.30. Our calculation indicated that recruitment of 25 participants would enable ICC estimation with CI precision of 0.26 for within-session analyses (three trials per testing session), and 0.33 for between-session analyses (mean of three trials are compared over two testing sessions) (see S1 Appendix for full calculation).”

Therefore, we feel no further action is required.

Which covariates were adjusted for, rationale for chosen tests, handling of missing data

Thank you for your comment. In accordance with the COnsensus-based Standards for the selection of health Measurement INstruments (COSMIN) Reporting Guideline (Gagnier et al, 2021), COSMIN Risk of Bias Tool (Mokkink et al, 2021) and its Guidance for Systematic Reviews of Patient-Reported Outcome Measures (Mokkink et al, 2024), adjustment for covariates is neither required nor recommended in studies assessing reliability. COSMIN advocates for the use of intraclass correlation coefficients (ICCs) to evaluate reliability for continuous data, which we deemed appropriate for our analysis. ICCs are designed to capture the proportion of total variance attributable to differences between subjects or clusters. Introducing covariate adjustment can artificially reduce this variance, resulting in context-specific estimates that may not accurately reflect the underlying reliability of the measure. For this reason, we followed COSMIN’s recommendation to report unadjusted ICCs. Adjusted ICCs may be appropriate in exploratory analyses or when assessing sources of heterogeneity, but they are not standard for primary reliability reporting.

In terms of the rationale for ICC model selection, we apologise that this is unclear. We have amended this and added a reference to support our choice (Koo and Li, 2016). This can be found in two locations: line 226, which now states:

“For a two-way mixed effects ICC model that is most suitable for a test-retest design used in this study , our calculation assumed a conservative expected correlation of 0.80, a moderate variance of 10 for between-participant test scores with no expected systematic differences, and aimed for a target CI width of 0.30.”

This was also reiterated this in the statistical analysis section (line 304):

“Two-way mixed effects ICC models are required for a test-retest design (39) used in this study, so within session ICCs3.1 and between session ICCs3.k were calculated with corresponding 95% CIs and interpreted as poor < 0.40; fair= 0.40- 0.70; good=0.70-0.90; excellent > 0.90 (40)”.

In terms of missing data, only one particpant withdrew and this is clearly stated in the results, but we have specifically amended this to clarify that there is no other missing data (line 326):

“Of twenty-six participants recruited, one withdrew due to pain during testing and was excluded from the analysis. In the remaining dataset, there were no instances of missing data.”

Tables/figures could be improved for clarity: labels are sometimes unclear; some redundancy exists between text and figures

Thank you for highlighting this. We agree that the units of measurement could be clearer. We have now amended the captions for Table 1, which now states (line 346):

“Table 1: Within-session descriptive statistics for peak force measurements, per device and testing day.”

The caption now states (line 352):

“Key: B-HHD = belt stabilised handheld dynamometer; FF = Forceframe; SD = standard deviation. Please note that all measurements are in newtons (N).”

The title of Table 2 has also been clarified and now states (Line 361):

“Table 2: Within-session ICCs3.1, 95%CIs, SEM and MDC estimates for both devices and limbs, according to testing day”.

Table 3 has also been clarified; on review of the submitted manuscript there appears to have been an issue with the text alignment. This has now been resolved and the title has now been amended, which now states (line 396):

“Table 3: Between-session descriptive statistics, ICCs, 95%CIs, SEM and MDC estimates for both devices, according to limb.”

Conclusions sometimes overstate findings relative to the data presented

Thank you for your feedback. We have taken care to try not to overstate the findings of the study, and this is especially important given the restricted generalisability due to the two-way mixed ICC models used.

Examples of where we have been careful with terminology include:

Line 496: “These findings tentatively suggest that different FF models may perform consistently across populations and hip muscle groups, though further studies are needed to confirm this.”

Line 514: “Collectively, these findings suggest that while both devices in our study are reliable for hip extension MVIC peak force measurement, the close consistency with other dynamometers, muscle groups and measurement parameters may be a function of the stable prone test position for hip extension, but

Attachment

Submitted filename: Response to reviewers.docx

pone.0328143.s007.docx (55KB, docx)

Decision Letter 1

Sohel Ahmed

12 Nov 2025

The test-retest reliability and agreement between a fixed frame and belt-stabilised handheld dynamometer for isometric hip flexion and extension peak force measurement in recreational cyclists

PONE-D-25-34937R1

Dear Dr. Hughes,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice will be generated when your article is formally accepted. Please note, if your institution has a publishing partnership with PLOS and your article meets the relevant criteria, all or part of your publication costs will be covered. Please make sure your user information is up-to-date by logging into Editorial Manager at Editorial Manager® and clicking the ‘Update My Information' link at the top of the page. For questions related to billing, please contact  and clicking the ‘Update My Information' link at the top of the page. For questions related to billing, please contact  and clicking the ‘Update My Information' link at the top of the page. For questions related to billing, please contact  and clicking the ‘Update My Information' link at the top of the page. For questions related to billing, please contact billing support....

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Sohel Ahmed, BPT, MPT, MDMR

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

-->Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.-->

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

**********

-->2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. -->

Reviewer #1: Yes

Reviewer #2: Yes

**********

-->3. Has the statistical analysis been performed appropriately and rigorously? -->

Reviewer #1: Yes

Reviewer #2: Yes

**********

-->4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.-->requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.-->

Reviewer #1: Yes

Reviewer #2: Yes

**********

-->5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.-->

Reviewer #1: Yes

Reviewer #2: Yes

**********

-->6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)-->

Reviewer #1: Thank you for resending the review. All Comments have been addressed and nothing to add to the previous comments.

Reviewer #2: (No Response)

**********

-->7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our Privacy Policy..-->..-->

Reviewer #1: Yes:Ahmed Ibrahim Al KharusiAhmed Ibrahim Al KharusiAhmed Ibrahim Al KharusiAhmed Ibrahim Al Kharusi

Reviewer #2: Yes:Esedullah AKARASEsedullah AKARASEsedullah AKARASEsedullah AKARAS

**********

Acceptance letter

Sohel Ahmed

PONE-D-25-34937R1

PLOS One

Dear Dr. Hughes,

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS One. Congratulations! Your manuscript is now being handed over to our production team.

At this stage, our production department will prepare your paper for publication. This includes ensuring the following:

* All references, tables, and figures are properly cited

* All relevant supporting information is included in the manuscript submission,

* There are no issues that prevent the paper from being properly typeset

You will receive further instructions from the production team, including instructions on how to review your proof when it is ready. Please keep in mind that we are working through a large volume of accepted articles, so please give us a few days to review your paper and let you know the next and final steps.

Lastly, if your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

You will receive an invoice from PLOS for your publication fee after your manuscript has reached the completed accept phase. If you receive an email requesting payment before acceptance or for any other service, this may be a phishing scheme. Learn how to identify phishing emails and protect your accounts at https://explore.plos.org/phishing.

If we can help with anything else, please email us at customercare@plos.org.

Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Dr. Sohel Ahmed

Academic Editor

PLOS One

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    S1 Appendix. Sample size calculation.

    (DOCX)

    pone.0328143.s001.docx (240KB, docx)
    S2 Appendix. Testing positions.

    (DOCX)

    S3 Appendix. Distribution of raw scores and residuals.

    (DOCX)

    pone.0328143.s003.docx (12.8MB, docx)
    S4 Appendix. Distribution of between device differences.

    (DOCX)

    pone.0328143.s004.docx (3.2MB, docx)
    S5 Appendix. Limits of agreement estimates.

    (DOCX)

    Attachment

    Submitted filename: Response to reviewers.docx

    pone.0328143.s007.docx (55KB, docx)

    Data Availability Statement

    An anonymised version of the dataset and Stata code is accessible via Manchester Metropolitan University’s data repository (E-space), and is freely available at https://doi.org/10.23634/MMU.00643463.


    Articles from PLOS One are provided here courtesy of PLOS

    RESOURCES