This article has been corrected: In Figure 6D, a splice was identified in the GLUT-1 blot. The authors explained that this occurred while combining two blots at different exposures of the same experiment. To address this, the authors provided the original uncropped blot for GLUT-1, which was used to generate the correction.
Figure 6. Effects of FGFR1 inhibition under FGF2 stimulation in serum-deprived H1703 cells in normoxic and hypoxic conditions.
H1703 cells, cultured in -FCS for 24 h, were pre-incubated for 1 h with 1 μM dovitinib or NVP-BGJ398, stimulated with FGF2, and then incubated in normoxic and hypoxic (0.5% O2) conditions. After 16 h glucose uptake (A), glycolysis (B), and lactate production (C) were measured and protein expression from cell lysates was assessed by Western Blot analysis (D). After 48 h cell proliferation (E) and cell death (F) were evaluated by cell counting with trypan blue dye exclusion method and by fluorescence microscopy on Hoechst 33342/PI stained cells, respectively. Results are mean values ±SD of three independent determinations. Data in (a, b, and c) are expressed as percent versus -FCS control cells in normoxia (-FCS N). *P < 0.05, **P < 0.01, ***P < 0.001 vs. -FCS N; ###P < 0.001 vs. FGF2 N, §§P < 0.01, §§§P < 0.001 vs. -FCS Hypoxia (-FCS H), +++P < 0.001 vs. FGF2 H. Data in (E) are expressed as percent versus FGF2 N. **P < 0.01, ***P < 0.001 vs. FGF2 N; ###P < 0.001 vs. FGF2 H; §P < 0.05, §§§P < 0.001 vs. FGF2+Dovitinib N.
The corrected Figure 6D is shown below. The authors declare that these corrections do not affect the results or conclusions of the paper.
Original article: Oncotarget. 2017; 8:91841–91859. 91841-91859. https://doi.org/10.18632/oncotarget.19279