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. 1977 Jan;264(1):49–62. doi: 10.1113/jphysiol.1977.sp011657

The regulation of the calcium conductance of cardiac muscle by adrenaline.

H Reuter, H Scholz
PMCID: PMC1307747  PMID: 839456

Abstract

1. The effect of adrenaline on the Ca-dependent slow inward current, Is, of mammalian cardiac muscle has been investigated by the voltage-clamp method. The mechanism of the increase in the conductance, gs, was analysed on the basis of a kinetic scheme (Hodgkin & Huxley, 1952) applicable to this system. 2. The rate constants alphad and betad, of activation of gs were not influenced by adrenaline, although the limiting conductance, gs, was greatly increased. 3. Reduction of [Ca]o from 1-8 to 0-2 mM decreased the amplitude of inward tail currents when gs was fully activated; however, the relative decrease of the current amplitude was the same with and without adrenaline. The reversal potential, ER, of Is was not changed by the drug. This indicates that the catecholamine has no influence on the selectivity of these conductance channels. 4. An increase in the number of functional conductance channels by adrenaline is discussed as a possible mechanism for the increase in Gs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. R., Stevens C. F. Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction. J Physiol. 1973 Dec;235(3):655–691. doi: 10.1113/jphysiol.1973.sp010410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Begenisich T., Stevens C. F. How many conductance states do potassium channels have? Biophys J. 1975 Aug;15(8):843–846. doi: 10.1016/S0006-3495(75)85858-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brady A. J. Physiological appraisial of the actions of catecholamines on myocardial contractions. Ann N Y Acad Sci. 1967 Feb 10;139(3):661–672. doi: 10.1111/j.1749-6632.1967.tb41236.x. [DOI] [PubMed] [Google Scholar]
  4. Brown H. F., McNaughton P. A., Noble D., Noble S. J. Adrenergic control of cardian pacemaker currents. Philos Trans R Soc Lond B Biol Sci. 1975 Jun 10;270(908):527–537. doi: 10.1098/rstb.1975.0029. [DOI] [PubMed] [Google Scholar]
  5. Fabiato A., Fabiato F. Relaxing and inotropic effects of cyclic AMP on skinned cardiac cells. Nature. 1975 Feb 13;253(5492):556–558. doi: 10.1038/253556b0. [DOI] [PubMed] [Google Scholar]
  6. GROSSMAN A., FURCHGOTT R. F. THE EFFECTS OF VARIOUS DRUGS ON CALCIUM EXCHANGE IN THE ISOLATED GUINEA-PIG LEFT AURICLE. J Pharmacol Exp Ther. 1964 Aug;145:162–172. [PubMed] [Google Scholar]
  7. George W. J., Ignarro L. J., Paddock R. J., White L., Kadowitz P. J. Oppositional effects of acetylcholine and isoproterenol on isometric tension and cyclic nucleotide concentrations in rabbit atria. J Cyclic Nucleotide Res. 1975;1(5):339–347. [PubMed] [Google Scholar]
  8. Giles W., Tsien R. W. Effects of acetylcholine on membrane currents in frog artrial muscle. J Physiol. 1975 Mar;246(2):64P–66P. [PubMed] [Google Scholar]
  9. Goldberg N. D., O'Dea R. F., Haddox M. K. Cyclic GMP. Adv Cyclic Nucleotide Res. 1973;3:155–223. [PubMed] [Google Scholar]
  10. HODGKIN A. L., HUXLEY A. F. A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol. 1952 Aug;117(4):500–544. doi: 10.1113/jphysiol.1952.sp004764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Katz B., Miledi R. The statistical nature of the acetycholine potential and its molecular components. J Physiol. 1972 Aug;224(3):665–699. doi: 10.1113/jphysiol.1972.sp009918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kaumann A. J., Birnbaumer L. Studies on receptor-mediated activation of adenylyl cyclases. IV. Characteristics of the adrenergic receptor coupled to myocardial adenylyl cyclase: stereospecificity for ligands and determination of apparent affinity constants for beta-blockers. J Biol Chem. 1974 Dec 25;249(24):7874–7885. [PubMed] [Google Scholar]
  13. Kirchberger M. A., Tada M., Repke D. I., Katz A. M. Cyclic adenosine 3',5'-monophosphate-dependent protein kinase stimulation of calcium uptake by canine cardiac microsomes. J Mol Cell Cardiol. 1972 Dec;4(6):673–680. doi: 10.1016/0022-2828(72)90120-4. [DOI] [PubMed] [Google Scholar]
  14. Kohlhardt M., Kübler M. The influence of metabolic inhibitors upon the transmembrane slow inward current in the mammalian ventricular myocardium. Naunyn Schmiedebergs Arch Pharmacol. 1975;290(2-3):265–274. doi: 10.1007/BF00510555. [DOI] [PubMed] [Google Scholar]
  15. Kukovetz W. R., Pöch G. The positive inotropic effect of cyclic AMP. Adv Cyclic Nucleotide Res. 1972;1:261–290. [PubMed] [Google Scholar]
  16. McDonald T. F., MacLeod D. P. Metabolism and the electrical activity of anoxic ventricular muscle. J Physiol. 1973 Mar;229(3):559–582. doi: 10.1113/jphysiol.1973.sp010154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Meinertz T., Nawrath H., Scholz H. Stimulatory effects of DB-c-AMP and adrenaline on myocardial contraction and 45Ca exchange. Experiments at reduced calcium concentration and low frequencies of stimulation. Naunyn Schmiedebergs Arch Pharmacol. 1973;279(4):327–338. doi: 10.1007/BF00500798. [DOI] [PubMed] [Google Scholar]
  18. Morad M., Rolett E. L. Relaxing effects of catecholamines on mammalian heart. J Physiol. 1972 Aug;224(3):537–558. doi: 10.1113/jphysiol.1972.sp009912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reuter H. Divalent cations as charge carriers in excitable membranes. Prog Biophys Mol Biol. 1973;26:1–43. doi: 10.1016/0079-6107(73)90016-3. [DOI] [PubMed] [Google Scholar]
  20. Reuter H. Localization of beta adrenergic receptors, and effects of noradrenaline and cyclic nucleotides on action potentials, ionic currents and tension in mammalian cardiac muscle. J Physiol. 1974 Oct;242(2):429–451. doi: 10.1113/jphysiol.1974.sp010716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reuter H., Scholz H. A study of the ion selectivity and the kinetic properties of the calcium dependent slow inward current in mammalian cardiac muscle. J Physiol. 1977 Jan;264(1):17–47. doi: 10.1113/jphysiol.1977.sp011656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reuter H. The dependence of slow inward current in Purkinje fibres on the extracellular calcium-concentration. J Physiol. 1967 Sep;192(2):479–492. doi: 10.1113/jphysiol.1967.sp008310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Reuter H. Uber die Wirkung von Adrenalin auf den cellulären Ca-Umsatz des Meerschweinchenvorhofs. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1965 Aug 20;251(4):401–412. [PubMed] [Google Scholar]
  24. Reuter H., Wollert U. Uber die Wirkung verschiedener sympathomimetischer Amine auf Kontraktionskraft und 45Ca-Aufnahme isolierter Meerschweinchenvorhöfe. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1967 Sep 15;258(3):288–296. [PubMed] [Google Scholar]
  25. Sperelakis N., Schneider J. A. A metabolic control mechanism for calcium ion influx that may protect the ventricular myocardial cell. Am J Cardiol. 1976 Jun;37(7):1079–1085. doi: 10.1016/0002-9149(76)90428-8. [DOI] [PubMed] [Google Scholar]
  26. Stevens C. F. Inferences about membrane properties from electrical noise measurements. Biophys J. 1972 Aug;12(8):1028–1047. doi: 10.1016/S0006-3495(72)86141-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sutherland E. W., Robison G. A. The role of cyclic-3',5'-AMP in responses to catecholamines and other hormones. Pharmacol Rev. 1966 Mar;18(1):145–161. [PubMed] [Google Scholar]
  28. Tsien R. W. Adrenaline-like effects of intracellular iontophoresis of cyclic AMP in cardiac Purkinje fibres. Nat New Biol. 1973 Sep 26;245(143):120–122. doi: 10.1038/newbio245120a0. [DOI] [PubMed] [Google Scholar]
  29. Tsien R. W., Giles W., Greengard P. Cyclic AMP mediates the effects of adrenaline on cardiac purkinje fibres. Nat New Biol. 1972 Dec 6;240(101):181–183. doi: 10.1038/newbio240181a0. [DOI] [PubMed] [Google Scholar]

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