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. 1977 Jan;264(1):125–139. doi: 10.1113/jphysiol.1977.sp011660

Calcium absorption in diphosphonate-treated rats: effect of parathyroid function, dietary calcium and phosphorus.

J P Bonjour, H Fleisch, U Trechsel
PMCID: PMC1307750  PMID: 402471

Abstract

1. The role of parathyroid hormone (PTH) and 1, 25-dihydroxy-cholecalciferol (1,25-(OH)2D3) in modulation of intestinal Ca absorption was studied in rats, using disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP), which is known to reduce 1,25-(OH)2D3 formation. 2. EHDP decreased intestinal Ca absorption. This effect could be abolished by small amounts of 1,25-(OH)2D3, whereas even large doses of PTH were ineffective. EHDP also decreased Ca absorption in thyroparathyroidectomized (TPTX) rats. Therefore the effect of EHDP on 1,25-(OH)2D3 production is unlikely to be mediated through PTH. 3. The correction by PTH of the decreased Ca absorption in TPTX rats was inhibited by EHDP. Since EHDP inhibits formation of 1,25-(OH)2D3 the effect of PTH on Ca absorption is likely to be mediated through this vitamin D3 metabolite. 4. In normal rats both a low Ca and a low P diet stimulated Ca absorption. In EHDP-treated intact rats low Ca still stimulated Ca absorption, whereas the effect of low P abolished. This indicates that low Ca and low P diets affect Ca absorption through different mechanisms. 5. Intestinal adaptation to a low Ca diet was still observed in EHDP-treated TPTX rats. Thus, in the rat, intestinal adaptation to low Ca diet can occur without PTH.

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Selected References

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  1. Baxter L. A., Deluca H. F., Bonjour J. P., Fleisch H. A. Inhibition of vitamin D metabolism by ethane-1-hydroxyl-1, 1-diphosphonate. Arch Biochem Biophys. 1974 Oct;164(2):655–662. doi: 10.1016/0003-9861(74)90077-0. [DOI] [PubMed] [Google Scholar]
  2. Bonjour J. P., DeLuca H. F., Fleisch H., Trechsel U., Matejowec L. A., Omdahl J. L. Reversal of the EHDP inhibition of calcium absorption by 1,25-dihydroxycholecalciferol. Eur J Clin Invest. 1973 Jan;3(1):44–48. doi: 10.1111/j.1365-2362.1973.tb00328.x. [DOI] [PubMed] [Google Scholar]
  3. Bonjour J. P., Trechsel U., Fleisch H., Schenk R., DeLuca H. F., Baxter L. A. Action of 1,25-dihydroxyvitamin D3 and a diphosphonate on calcium metabolism in rats. Am J Physiol. 1975 Aug;229(2):402–408. doi: 10.1152/ajplegacy.1975.229.2.402. [DOI] [PubMed] [Google Scholar]
  4. Boyle I. T., Gray R. W., DeLuca H. F. Regulation by calcium of in vivo synthesis of 1,25-dihydroxycholecalciferol and 21,25-dihydroxycholecalciferol. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2131–2134. doi: 10.1073/pnas.68.9.2131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boyle I. T., Miravet L., Gray R. W., Holick M. F., Deluca H. F. The response of intestinal calcium transport to 25-hydroxy and 1,25-dihydroxy vitamin D in nephrectomized rats. Endocrinology. 1972 Mar;90(3):605–608. doi: 10.1210/endo-90-3-605. [DOI] [PubMed] [Google Scholar]
  6. CRAMER C. F. Participation of parathyroid glands in control of calcium absorption in dogs. Endocrinology. 1963 Feb;72:192–196. doi: 10.1210/endo-72-2-192. [DOI] [PubMed] [Google Scholar]
  7. Garabedian M., Holick M. F., Deluca H. F., Boyle I. T. Control of 25-hydroxycholecalciferol metabolism by parathyroid glands. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1673–1676. doi: 10.1073/pnas.69.7.1673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gasser A. B., Morgan D. B., Fleisch H. A., Richelle L. J. The influence of two diphosphonates on calcium metabolism in the rat. Clin Sci. 1972 Jul;43(1):31–45. doi: 10.1042/cs0430031. [DOI] [PubMed] [Google Scholar]
  9. Hill L. F., Lumb G. A., Mawer E. B., Stanbury S. W. Indirect inhibition of the biosynthesis of 1,25-dihydroxycholecalciferol in rats treated with a diphosphonate. Clin Sci. 1973 Apr;44(4):335–347. doi: 10.1042/cs0440335. [DOI] [PubMed] [Google Scholar]
  10. KIMBERG D. V., SCHACHTER D., SCHENKER H. Active transport of calcium by intestine: effects of dietary calcium. Am J Physiol. 1961 Jun;200:1256–1262. doi: 10.1152/ajplegacy.1961.200.6.1256. [DOI] [PubMed] [Google Scholar]
  11. Morgan D. B., Bonjour J. P., Gasser A. B., O'Brien K., Fleisch H. A. The influence of a diphosphonate on the intestinal absorption of calcium. Isr J Med Sci. 1971 Mar;7(3):384–386. [PubMed] [Google Scholar]
  12. Morrissey R. L., Wasserman R. H. Calcium absorption and calcium-binding protein in chicks on differing calcium and phosphorus intakes. Am J Physiol. 1971 May;220(5):1509–1515. doi: 10.1152/ajplegacy.1971.220.5.1509. [DOI] [PubMed] [Google Scholar]
  13. RASMUSSEN H. The influence of parathyroid function upon the transport of calcium in isolated sacs of rat small intestine. Endocrinology. 1959 Sep;65:517–519. doi: 10.1210/endo-65-3-517. [DOI] [PubMed] [Google Scholar]
  14. Sammon P. J., Stacey R. E., Bronner F. Role of parathyroid hormone in calcium homeostasis and metabolism. Am J Physiol. 1970 Feb;218(2):479–485. doi: 10.1152/ajplegacy.1970.218.2.479. [DOI] [PubMed] [Google Scholar]
  15. Schenk R., Merz W. A., Mühlbauer R., Russell R. G., Fleisch H. Effect of ethane-1-hydroxy-1,1-diphosphonate (EHDP) and dichloromethylene diphosphonate (Cl 2 MDP) on the calcification and resorption of cartilage and bone in the tibial epiphysis and metaphysis of rats. Calcif Tissue Res. 1973 Mar 12;11(3):196–214. doi: 10.1007/BF02547219. [DOI] [PubMed] [Google Scholar]
  16. Shah B. G., Draper H. H. Depression of calcium absorption in parathyroidectomized rats. Am J Physiol. 1966 Oct;211(4):963–966. doi: 10.1152/ajplegacy.1966.211.4.963. [DOI] [PubMed] [Google Scholar]
  17. Tanaka Y., Deluca H. F. The control of 25-hydroxyvitamin D metabolism by inorganic phosphorus. Arch Biochem Biophys. 1973 Feb;154(2):566–574. doi: 10.1016/0003-9861(73)90010-6. [DOI] [PubMed] [Google Scholar]
  18. Taylor C. M., Mawer E. B., Reeve A. The effects of a diphosphonate and dietary calcium on the metabolism of vitamin D3 (cholecalciferol) in the chick. Clin Sci Mol Med. 1975 Nov;49(5):391–400. doi: 10.1042/cs0490391. [DOI] [PubMed] [Google Scholar]

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