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. 2026 Apr 15;21(4):e0347252. doi: 10.1371/journal.pone.0347252

Effect of an educational program on the knowledge, attitudes, and practices of hospital nurses regarding evidence-based practice: A quasi-experimental study

Omed Hamarasheed Mehammed-Ameen 1,2,*,#, Salih Ahmed Abdulla 1,3,#
Editor: Rohit Ravi4
PMCID: PMC13082721  PMID: 41984911

Abstract

Background

Preparing nurses for evidence-based practice (EBP) requires developing their knowledge, skills, attitudes, and ability to apply evidence-based practice in clinical settings. This study assessed the effects of an educational program on hospital nurses’ knowledge, attitudes, and practices regarding evidence-based practice.

Methods

A quasi-experimental study with a control group and pre–post intervention assessments. The study was conducted among 116 hospital nurses in Kirkuk City, Iraq, selected using convenience sampling (58 in the intervention group and 58 in the control group). Nurses in the intervention group participated in an educational program on evidence-based practice. Data were collected at baseline and three months post-intervention using a structured self-administered questionnaire assessing knowledge, attitudes, and self-reported practice.

Results

Nurses in the intervention group demonstrated statistically significant improvements in evidence-based practice knowledge, attitudes, and practices compared with baseline, while no significant changes were observed in the control group (p < 0.05). Repeated-measures ANOVA revealed a significant group × time interaction, with large effects on knowledge (η² = 0.85) and practice (η² = 0.69), and a moderate effect on attitudes toward evidence-based practice (η² = 0.13).

Conclusion

The educational program was associated with significant improvements in nurses’ EBP knowledge, attitudes, and self-reported practices. These findings suggest that structured EBP education can enhance nursing competencies and support the integration of evidence-based care into clinical practice.

Introduction

Evidence-based practice (EBP) is now a fundamental part of modern nursing, integrating the best available research evidence with clinical expertise and patients’ values to support informed decision-making, enhance care quality, and improve patient outcomes [1,2]. By promoting the systematic use of research findings in clinical care, EBP enables nursing practice to move beyond tradition and routine toward more accountable and effective healthcare delivery [3]. Despite its established benefits, the integration of EBP into routine nursing practice remains inconsistent across healthcare systems. Nurses frequently encounter multiple barriers, including limited knowledge and skills related to evidence appraisal, insufficient training opportunities, time constraints, and inadequate organizational support [4,5]. Furthermore, difficulties in interpreting and critically appraising research literature often lead nurses to rely on personal experience or informal peer consultation rather than on formal evidence sources, such as peer-reviewed studies and clinical guidelines [68]. These challenges contribute to a persistent gap between evidence generation and its application in clinical nursing practice.

To address these gaps, educational interventions and structured training programs have been widely implemented as strategies to strengthen nurses’ EBP competencies across diverse healthcare settings [1,2,9]. Evidence from systematic reviews and meta-analyses consistently demonstrates that such interventions can significantly improve nurses’ EBP-related knowledge, attitudes, skills, and self-efficacy.

However, the sustainability of these improvements and their translation into routine clinical practice remain ongoing challenges, particularly in healthcare systems where EBP is not firmly embedded within organizational culture or professional education [911]. In low- and middle-income countries, these challenges are often amplified by structural and educational constraints. In Iraq, to date, no empirical studies have evaluated the effectiveness of educational programs designed to enhance nurses’ EBP competencies.

Although the Iraqi healthcare system has recently demonstrated growing institutional commitment to improving quality of care through the adoption of evidence-based standards and clinical protocols, there is still no explicit national policy mandating nurses to implement evidence-based practice (EBP) as a statutory requirement. Moreover, insufficient integration of EBP content into undergraduate nursing curricula leaves many nurses inadequately prepared to search for, critically appraise, and apply research evidence in clinical practice. As a result, EBP implementation in Iraqi healthcare settings remains limited. Therefore, there is a critical need for context-specific evidence to determine whether structured educational interventions can effectively strengthen EBP competencies among hospital nurses in Iraq.

This study is informed by adult learning and EBP theories, which posit that nurses are more likely to adopt EBP when education enhances both their research knowledge and their attitudes toward its clinical application. Accordingly, educational programs are viewed as a central mechanism for strengthening EBP competence by fostering knowledge, positive attitudes, and evidence-informed decision-making [4,5]. The framework further assumes a close interrelationship among knowledge, attitudes, and practices, whereby improvements in understanding and attitudes support the integration of evidence into routine care. This perspective guided the design of the educational intervention, the selection of study outcomes, and the analysis of relationships among knowledge, attitudes, and practices [1,12,13].

Accordingly, this quasi-experimental study aims to evaluate the effect of a structured educational intervention on hospital nurses’ knowledge, attitudes, and practices regarding EBP in Kirkuk City, Iraq. A secondary objective is to examine the relationships among these domains to determine whether improvements in knowledge and attitudes are associated with positive changes in EBP practices following the intervention. By generating context-specific evidence from an under-researched setting, this study contributes to national efforts to enhance nursing practice quality and supports the development of educational and institutional strategies for scaling effective EBP training models in resource-constrained healthcare systems.

Materials and methods

Design and setting of the study

A quasi-experimental design with a control group was used to examine the effect of an educational program on hospital nurses’ knowledge, attitudes, and practices related to evidence-based practice in Kirkuk City, Iraq. Data were collected at baseline and three months post-intervention. Four governmental hospitals were purposively selected and assigned to either the intervention group (Azadi Teaching Hospital and Maternity and Delivery Hospital) or the control group (Kirkuk General Hospital and Paediatric Hospital). The study included bachelor-prepared nurses from various clinical units, capturing staff involved in a range of patient care activities.

Participants and sampling

The participant flow through the study is presented in “Fig 1”. The study initially targeted all bachelor-prepared nurses (N = 464) working in different clinical units across the selected hospitals. To account for potential dropouts, 120 nurses were recruited (60 intervention, 60 control) using a nonrandomized convenience sampling method. Four nurses (two per group) withdrew, resulting in a final sample of 116 (58 per group). Inclusion criteria were a bachelor’s degree and willingness to participate, while nurses with less than one year of experience or those on extended leave were excluded. The sample size was calculated using G*Power software for repeated-measures ANOVA. A total of 84 nurses was required to detect a small-to-moderate effect size (f = 0.20) with 95% power at a significance level of α = 0.05. The selected effect size was informed by prior evaluations of educational interventions in EBP, which have reported comparable small-to-moderate effects on nurses’ knowledge, attitudes, and practices [2,14].

Fig 1. Participant flow diagram of the study.

Fig 1

A diagram showing the enrolment, allocation, follow-up, and analysis of nurses in the intervention and control groups throughout the study period.

Data collection and study instruments

Several procedural steps were implemented to minimize the risk of contamination between the intervention and control groups. First, allocation was conducted at the hospital level to prevent interaction between participants from different study arms within the same institution. The participating hospitals operate under separate administrative structures, and no formal staff rotation or reassignment occurred between hospitals during the study period. Second, the educational intervention was delivered exclusively outside the hospitals at the College of Nursing, University of Kirkuk, and training materials were not distributed beyond this site. Third, data collection in both groups was conducted within the same predefined timeframe to reduce the likelihood of delayed information transfer.

Data were collected between January and May 2025. After obtaining informed consent and explaining the study purpose, nurses in both the intervention and control groups completed a self-administered questionnaire, which required approximately 20–25 minutes to complete. Following baseline data collection, nurses in the intervention group participated in a two-week intensive educational program on evidence-based practice (EBP). Three months after completion of the intervention, participants in both groups completed the same questionnaire to assess changes in EBP-related knowledge, attitudes, and practices. Pre- and post-intervention scores were compared to evaluate the effect of the educational program.

Data were collected using a structured self-administered questionnaire developed through an extensive literature review and adapted from two validated instruments: the Evidence-Based Practice Questionnaire for Nurses (EBPQ) by Upton and Upton [15] and the Evidence-Based Practice Competency Questionnaire–Professional Version (EBP-COQ prof©) by Ruzafa-Martinez, Lopez-Iborra [16]. Minor modifications were made to ensure contextual relevance to Iraqi hospital settings. The questionnaire comprised two sections: sociodemographic and occupational characteristics and 36 items assessing EBP competencies across three subscales including knowledge (11 items), attitudes (15 items), and practices (10 items). Knowledge items were rated on a 7-point scale ranging from 1 (poor) to 7 (good), while attitudes and practices were rated on 5-point Likert scales ranging from 1 (strongly disagree/never) to 5 (strongly agree/always).

Content validity was established by a panel of 12 experts in nursing education, research, and evidence-based practice, who evaluated the questionnaire for clarity, relevance, and comprehensiveness. Minor revisions were made based on their feedback. Internal consistency reliability was assessed through a pilot study involving 20 nurses who were not included in the main study, yielding Cronbach’s alpha coefficients of 0.73 for the knowledge subscale, 0.72 for attitudes, and 0.80 for practices, indicating acceptable reliability.

Educational program

An educational program titled “Evidence-Based Nursing Practice” was developed following an extensive review of the literature and validated prior to implementation. It was structured as a multicomponent intervention aimed at strengthening nurses’ EBP competencies and was informed by established EBP models and evidence from effective educational programs [2,17].

The program was designed and delivered by one professor and two assistant professors from the College of Nursing, University of Kirkuk, all with expertise in evidence-based practice and nursing research. Participants in the intervention group were released from clinical duties during the training period and were divided into two cohorts, each completing the program over two weeks. The intervention comprised ten sessions of approximately three hours each (totalling 30 hours), integrating theoretical instruction with practical application. Participants were required to attend a minimum of nine sessions to be included in the intervention analysis. Attendance was recorded for each session to ensure compliance.

Program content followed the core EBP steps, including formulating PICO questions, searching electronic databases, critically appraising evidence, integrating findings with clinical expertise and patient preferences, and evaluating outcomes [18]. Teaching strategies combined lectures, self-directed learning, hands-on database searching, small-group workshops, case-based discussions, and mentoring, consistent with evidence supporting interactive and blended learning approaches [1,12,17]. Attendance was monitored, and formative assessments were used to reinforce learning. The control group did not receive any EBP-related education during the study period.

Data analysis

Data were analyzed using SPSS version 27. Descriptive statistics (frequencies, percentages, means, and standard deviations) summarized the sample characteristics. Inferential analyses included chi-square and Fisher’s exact tests for categorical variables and paired-sample t tests for within-group comparisons. A repeated-measures ANOVA (Time × Group) examined the effects of time, group, and their interaction on knowledge, attitudes, and practice scores. Statistical significance was set at p ≤ 0.05, and effect sizes were reported using Partial Eta Squared (η²), interpreted as small (0.01), medium (0.06), and large (0.14) following Cohen (1988). Pearson correlation coefficients assessed associations among the EBP domains.

Ethical consideration

Ethical approval was obtained from the Research Ethics Committee of the College of Nursing, Hawler Medical University, Erbil, Iraq (Approval No. 246; 6 June 2024), and from the Research Committee of the Knowledge Management and Research Division, Kirkuk Governorate Health Department (Reference No. 551; 8 July 2024), permitting the study’s implementation in government hospitals in Kirkuk. Authorization was also secured from hospital administrations and nursing managers, including approval for nurses in the intervention group to attend the educational program and to be released from clinical duties during the training period.

Verbal informed consent was obtained from all participants after providing a detailed explanation of the study objectives, procedures, potential risks and benefits, confidentiality, and the voluntary nature of participation, including the right to withdraw at any time without penalty. The use of verbal consent was explicitly reviewed and approved by the Research Ethics Committee due to the minimal-risk nature of the study. Consent was documented by the researcher using a standardized consent record form, which was signed and dated by the data collector to confirm that informed consent had been obtained.

Confidentiality was maintained through coded questionnaires, with no identifiable information collected, and all procedures adhered to the principles of the Declaration of Helsinki.

Results

Characteristics of the sample

A total of 116 participants were enrolled in the study, with 58 assigned to the control group and 58 assigned to the intervention group. Sociodemographic and occupational characteristics were collected from both groups of the study. The results show that there was no statistically significant difference in age, sex, marital status, years of experience, awareness regarding EBP, and participation in events or training related to EBP between the control group and the intervention group (Table 1).

Table 1. Sociodemographic and occupational characteristics of the sample (116).

Variables Control (n = 58) Intervention (n = 58) P Value
Age in years [Mean (SD)] 27.43 (± 3.42) 27.97 (± 3.76) 0.808
Sex [F (%)]
Male 22 (37.9) 19 (32.8) 0.560

Female 36 (62.1) 39 (67.2)
Marital Status [F (%)]
Single 36 (62.1) 33 (56.9) 0.544



Married 22 (37.9) 24 (41.4)
Divorced 0 (0) 1 (1.7)
Hospital [F (%)]
Azadi teaching hospital 42 (72.4)

Al-Naser hospital 16 (27.6)
Kirkuk general hospital 47 (81)
Paediatric hospital 11 (19)
Experience in years [Mean (SD)] 3.71 (± 2.12) 4.53 (± 3.25) 0.553
Have you ever heard about Evidence-based nursing practice? [F (%)]
Yes 33 (56.9) 29 (50) 0.543

No 25 (43.1) 29 (50)
Participation in events or training related to EBP? [F (%)]
Yes 6 (10.3) 4 (6.9) 0.743

No 52 (89.7) 54 (93.1)

- F = Frequency

- SD = Standard Deviation

Changes in participants’ EBP knowledge, attitudes, and practice scores after the intervention

In Table 2, paired-samples t tests were conducted separately for the control and intervention groups to determine the effects of the educational program on the participants’ knowledge, attitudes, and practice scores. In terms of knowledge, the control group showed no significant difference between the pre-intervention (mean = 2.08) and post-intervention (mean = 2.10) results, with p = 0.32. The intervention group demonstrated a noteworthy increase in knowledge from pre-intervention (mean = 2.06) to the post-intervention (mean = 5.07), with a p value of less than 0.001.

Table 2. Changes in participants’ EBP knowledge, attitudes, and practice scores after the intervention.

Variables

(Score range)
Pre

Mean (± SD)
Post

Mean (± SD)
MD (95% CI) SMD (95% CI) T P value
Knowledge (1 –7)
Control 2.08 (± 0.41) 2.10 (± 0.34) −0.02 (−0.07, 0.02) −0.13 (−0.39, 0.13) −1.00 0.32
Intervention 2.06 (± 0.35) 5.07 (± 0.83) −3.00 (−3.24, −2.77) −3.42 (−4.09, −2.74) −26.03 <0.001
Attitudes (1 –5)
Control 3.50 (± 0.31) 3.56 (± 0.31) −0.06 (−0.14, 0.03) −0.18 (−0.44, 0.08) −1.37 0.18
Intervention 3.69 (± 0.36) 4.04 (± 0.31) −0.34 (−0.46, −0.23) −0.78 (−1.07, −0.49) −5.96 <0.001
Practice (1 –5)
Control 2.05 (± 0.60) 2.08 (± 0.63) −0.03 (−0.06, 0.00) −0.23 (−0.49, 0.03) −1.76 0.08
Intervention 2.00 (± 0.59) 3.71 (± 0.51) −1.71 (−1.92, −1.50) −2.17 (−2.63, −1.69) −16.48 <0.001

- SD = Standard deviation, MD = Mean Difference, SMD = Standardized Mean Difference, CI = Confidence Interval, t = paired t-test

In terms of attitudes, the control group showed no significant change between the pre-intervention (mean = 3.50) and post-intervention (mean = 3.56), with p = 0.18. However, the intervention group exhibited a significant positive change in attitudes, with scores increasing from the pre-intervention (mean = 3.69) to the post-intervention (mean = 4.04), with p < 0.001.

For practice, the control group showed no significant difference between the pre-intervention (mean = 2.05) and post-intervention (mean = 2.08) results, with p = 0.08. In contrast, the intervention group showed a statistically significant increase in scores from the pre-intervention (mean = 2.00) to the post-intervention (mean = 3.71), with p < 0.001.

Effects of intervention on participants’ EBP knowledge, attitudes, and practices

The repeated-measures ANOVA (Table 3) showed significant effects of the educational program on knowledge, attitudes, and practices regarding EBP. Knowledge improved significantly over time (F = 658.47, p < 0.001, η² = 0.85) and between groups (F = 365.96, p < 0.001, η² = 0.76), with a strong time–group interaction (F = 637.06, p < 0.001, η² = 0.85), indicating that the program’s effect varied by group and time.

Table 3. Effects of the educational program on participants’ knowledge, attitudes, and practices regarding EBP (Repeated-Measures ANOVA).

Variable Source F df P value n2 (Effect size)
Knowledge Time 658.47 1, 114 <0.001 0.85 (large)
Group 365.96 1, 114 <0.001 0.76 (large)
Time * Group 637.06 1, 114 <0.001 0.85 (large)
Attitudes Time 32.05 1, 114 <0.001 0.22 (large)
Group 45.79 1, 114 <0.001 0.29 (large)
Time * Group 16.64 1, 114 <0.001 0.13 (medium)
Practice Time 274.53 1, 114 <0.001 0.71 (large)
Group 68.97 1, 114 <0.001 0.38 (large)
Time * Group 258.43 1, 114 <0.001 0.69 (large)

- F = F-static, df = degree of freedom, n2= partial eta squared

- Mauchly’s test indicated that the assumption of sphericity was met for all within-subjects effects, W = 1.000, p > .05.

- All reported effects were statistically significant at p < 0.001

Attitudes toward EBP also improved over time (F = 32.05, p < 0.001, η² = 0.22) and differed significantly across groups (F = 45.79, p < 0.001, η² = 0.29), with a moderate time–group interaction (F = 16.42, p < 0.001, η² = 0.13).

Practice scores increased markedly over time (F = 274.53, p < 0.001, η² = 0.71) and between groups (F = 68.97, p < 0.001, η² = 0.38), with a strong time–group interaction (F = 258.4, p < 0.001, η² = 0.69), showing that the program effects on practice were shaped by both time and group.

Correlation among post intervention EBP domain scores (Intervention group)

Pearson correlation analysis showed significant positive associations among EBP domains (Table 4). Total EBP correlated very strongly with knowledge (r = .913, p < .001) and strongly with practice (r = .853, p < .001). Moderate correlations were observed with attitudes (r = .553, p < .001) and between knowledge and practice (r = .638, p < .001). Attitudes correlated moderately with practice (r = .400, p = .002), while knowledge and attitudes showed a weak but significant association (r = .291, p = .027).

Table 4. Pearson correlation coefficients among post intervention EBP domain scores (Intervention group).

Items Total EBP Mean score Knowledge Mean score Attitudes Mean score Practice Mean score
Intervention Group
Total EBP Mean score Pearson Correlation 1
N 58
Knowledge Mean score Pearson Correlation .913** 1
P- value 0.000
N 58 58
Attitudes Mean score Pearson Correlation .553** .291* 1
P- value 0.000 0.027
N 58 58 58
Practice Mean score Pearson Correlation .853** .638** .400** 1
P- value 0.000 0.000 0.002
N 58 58 58 58

**. Correlation is significant at the 0.01 level (2-tailed).

*. Correlation is significant at the 0.05 level (2-tailed).

Discussion

This study is the first conducted in Iraq to evaluate the impact of a structured educational program on hospital nurses’ knowledge, attitudes, and practices related to evidence-based practice (EBP). The findings demonstrate statistically significant improvements across all three domains among nurses who participated in the intervention, with no comparable changes observed in the control group. These results provide context-specific evidence supporting the effectiveness of structured EBP education. Such programs strengthen nurses’ competencies and facilitate the integration of evidence-based approaches into routine clinical practice.

At baseline, nurses in both groups exhibited limited EBP knowledge and practice despite holding moderately positive attitudes toward EBP. This pattern is consistent with international evidence indicating that nurses often value EBP. However, they lack the knowledge, skills, and experiential preparation required for its application in clinical settings. Such gaps are commonly attributed to insufficient emphasis on EBP in pre-registration education and limited access to structured in-service training, particularly in resource-constrained healthcare systems [1,19,20]. These findings underscore the need for targeted educational interventions that address both theoretical understanding and practical implementation of EBP.

The observed improvements following the intervention align with evidence from experimental and quasi-experimental studies and meta-analyses demonstrating that theory-informed, multifaceted educational approaches are effective in enhancing nurses’ EBP-related knowledge, attitudes, and practice behaviors. Interventions that incorporate interactive learning strategies, practical skill development, and clinically relevant applications have consistently been shown to outperform usual education or no intervention in promoting EBP competence [2,14,17,19]. The present findings extend this evidence to the Iraqi context and support the transferability of such educational models across diverse healthcare settings.

The timing of outcome assessment is important when interpreting the impact of EBP educational interventions. In this study, outcomes assessed three months after program completion showed significant improvements, consistent with evidence that benefits often emerge after a period of clinical application and reflection. Similar patterns have been reported in prior studies, where improvements were not immediate but appeared at later follow-up before declining [21]. Collectively, these findings suggest that EBP education can yield meaningful short- to mid-term gains while highlighting the importance of ongoing reinforcement and organizational support to sustain long-term improvements [14,2123]

The significant group × time effects observed in the repeated-measures ANOVA further strengthen causal attribution to the educational program by demonstrating differential trajectories between the intervention and control groups. This pattern indicates that the observed changes were unlikely to result from maturation or external influences. Consistent with findings from randomized and controlled trials, adequately dosed, multimodal EBP training has been shown to produce sustained improvements in EBP competence and implementation over several months [11,14,2123]. These results reinforce the methodological robustness of the intervention and its role in driving observed improvements.

The observed effect size for knowledge (η² = 0.85) indicates a very large proportion of variance explained by the intervention. While such magnitudes are uncommon in educational research, several factors may contribute, including low baseline knowledge, close alignment of the outcome measure with the intervention, and high sensitivity to change. Nevertheless, this large effect size should be interpreted with caution, as it may also reflect the quasi-experimental design, cluster-level influences, reliance on self-reported measures, potential response bias, and limited within-group variability, rather than the intervention effect alone.

It is important to acknowledge that the assessment of EBP practice was based on self-reported questionnaire responses, which may be subject to social desirability and response bias. Therefore, the observed improvements likely reflect perceived or reported behavior rather than objectively verified clinical practice. Future studies should incorporate objective assessment methods, such as clinical audits, direct observation, or patient-level outcome measures, to more accurately evaluate changes in evidence-based practice implementation.

Finally, the strong positive correlations observed among post-intervention knowledge, attitudes, and practice scores in the intervention group support the theoretical assumption that these domains are closely interconnected. This finding aligns with conceptual models and empirical evidence indicating that higher levels of education, structured EBP training, and positive beliefs about EBP are key determinants of nurses’ evidence-based behaviors and their use of research evidence in clinical decision-making [20,2428]. Together, these results suggest that educational interventions that simultaneously target knowledge and attitudes may be particularly effective in promoting sustained EBP practice.

Limitations

The quasi-experimental design without randomization limits causal inference between the educational intervention and observed changes in knowledge, attitudes, and practices. Hospital-level allocation without random assignment may have introduced residual cluster-level confounding, as unmeasured institutional factors (e.g., organizational culture, leadership, staffing, and workload) may have influenced outcomes despite comparable baseline characteristics. The use of convenience sampling and inclusion of only bachelor-prepared nurses from four governmental hospitals in a single city limits generalizability, and findings may not apply to diploma nurses, private-sector hospitals, or other regions of Iraq. Volunteer bias may also have occurred, as participants may have been more motivated toward EBP, potentially inflating the intervention effects. Outcomes were based on self-reported questionnaires, which are subject to response and social desirability bias. Finally, the lack of long-term follow-up limits conclusions regarding the sustainability of the intervention’s effects.

Conclusion

This study found that participation in a structured educational program was associated with significant short-term improvements in hospital nurses’ knowledge, attitudes, and self-reported practices related to evidence-based practice in Kirkuk City, Iraq. While the findings suggest that structured EBP education may enhance nurses’ competencies, the results should be interpreted in light of the quasi-experimental design and reliance on self-reported practice measures. Integrating EBP-focused content into continuing professional development and undergraduate nursing curricula, supported by enabling institutional environments, may contribute to strengthening evidence-based care. Further longitudinal and randomized research incorporating objective outcome measures is warranted to confirm the magnitude and sustainability of these effects.

Supporting information

S1 Data. De-identified dataset underlying the findings of this study.

(XLSX)

pone.0347252.s001.xlsx (48.3KB, xlsx)

Acknowledgments

The researchers appreciate all participating nurses for their time and cooperation, and they extend special thanks to the teachers of the College of Nursing at the University of Kirkuk for their contributions in designing and implementing the educational program.

Data Availability

All relevant data are within the paper and its Supporting Information files.

Funding Statement

The author(s) received no specific funding for this work.

References

  • 1.Sapri ND, Ng YT, Wu VX, Klainin-Yobas P. Effectiveness of educational interventions on evidence-based practice for nurses in clinical settings: A systematic review and meta-analysis. Nurse Educ Today. 2022;111:105295. doi: 10.1016/j.nedt.2022.105295 [DOI] [PubMed] [Google Scholar]
  • 2.Vaajoki A, Kvist T, Kulmala M, Tervo-Heikkinen T. Systematic education has a positive impact on nurses’ evidence-based practice: Intervention study results. Nurse Educ Today. 2023;120:105597. doi: 10.1016/j.nedt.2022.105597 [DOI] [PubMed] [Google Scholar]
  • 3.Chao W-Y, Huang L-C, Hung H-C, Hung S-C, Chuang T-F, Yeh L-Y, et al. Effectiveness of Digital Flipped Learning Evidence-Based Practice on Nurses’ Knowledge, Attitude, and Practice: A Quasi-Experimental Trial. Healthcare (Basel). 2022;10(7):1257. doi: 10.3390/healthcare10071257 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Qtait M. Nurses’ Knowledge, Attitudes, and Implementation of Evidence-Based Practice Comparative Study. SAGE Open Nurs. 2025;11. doi: 10.1177/23779608251343611 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Ejheisheh MA, Ayed A, Aqtam I, Batran A, Farajallah M. Assessing knowledge, attitudes, and skills toward evidence-based practice among Palestinian nursing students. BMC Nurs. 2025;24(1):282. doi: 10.1186/s12912-025-02927-y [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Kumah EA, McSherry R, Bettany-Saltikov J, van Schaik P. Evidence-informed practice: simplifying and applying the concept for nursing students and academics. Br J Nurs. 2022;31(6):322–30. doi: 10.12968/bjon.2022.31.6.322 [DOI] [PubMed] [Google Scholar]
  • 7.Shayan SJ, Kiwanuka F, Nakaye Z. Barriers associated with evidence-based practice among nurses in low- and middle-income countries: A systematic review. Worldviews Evid Based Nurs. 2019;16(1):12–20. [DOI] [PubMed] [Google Scholar]
  • 8.Lunden A, Kvist T, Teräs M, Häggman-Laitila A. Readiness and leadership in evidence-based practice and knowledge management: A cross-sectional survey of nurses’ perceptions. Nordic Journal of Nursing Research. 2020;41(4):187–96. doi: 10.1177/2057158520980687 [DOI] [Google Scholar]
  • 9.Pereira F, Lehmann-Wellig B, Verloo H. Enhancing beliefs and implementation of evidence-based practice among undergraduate nurses using a multi-component educational programme: a pre-post study. BMC Med Educ. 2025;25(1):531. doi: 10.1186/s12909-025-07121-x [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Fontaine G, Vinette B, Weight C, Maheu-Cadotte M-A, Lavallée A, Deschênes M-F, et al. Effects of implementation strategies on nursing practice and patient outcomes: a comprehensive systematic review and meta-analysis. Implement Sci. 2024;19(1):68. doi: 10.1186/s13012-024-01398-0 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Jeong D, Park C, Sugimoto K, Jeon M, Kim D, Eun Y. Effectiveness of an Evidence-Based Practice Education Program for Undergraduate Nursing Students: A Systematic Review and Meta-Analysis. Int J Environ Res Public Health. 2024;21(5):637. doi: 10.3390/ijerph21050637 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Portela Dos Santos O, Melly P, Hilfiker R, Giacomino K, Perruchoud E, Verloo H, et al. Effectiveness of Educational Interventions to Increase Skills in Evidence-Based Practice among Nurses: The EDITcare Systematic Review. Healthcare (Basel). 2022;10(11):2204. doi: 10.3390/healthcare10112204 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Bhatarasakoon P, Chiaranai C, Lockwood C. Undergraduate knowledge needs: Pedagogy for evidence-based nursing and allied health. Nurs Health Sci. 2024;26(1):e13105. doi: 10.1111/nhs.13105 [DOI] [PubMed] [Google Scholar]
  • 14.Gallagher-Ford L, Koshy Thomas B, Connor L, Sinnott LT, Melnyk BM. The Effects of an Intensive Evidence-Based Practice Educational and Skills Building Program on EBP Competency and Attributes. Worldviews Evid Based Nurs. 2020;17(1):71–81. doi: 10.1111/wvn.12397 [DOI] [PubMed] [Google Scholar]
  • 15.Upton D, Upton P. Development of an evidence-based practice questionnaire for nurses. J Adv Nurs. 2006;53(4):454–8. doi: 10.1111/j.1365-2648.2006.03739.x [DOI] [PubMed] [Google Scholar]
  • 16.Ruzafa-Martinez M, Lopez-Iborra L, Moreno-Casbas T, Madrigal-Torres M. Development and validation of the competence in evidence based practice questionnaire (EBP-COQ) among nursing students. BMC Med Educ. 2013;13:19. doi: 10.1186/1472-6920-13-19 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Liu M, Lin Y, Dai Y, Deng Y, Chun X, Lv Y, et al. A multi-dimensional EBP educational program to improve evidence-based practice and critical thinking of hospital-based nurses: Development, implementation, and preliminary outcomes. Nurse Educ Pract. 2021;52:102964. doi: 10.1016/j.nepr.2020.102964 [DOI] [PubMed] [Google Scholar]
  • 18.Melnyk BM, Fineout-Overholt. Evidence-based practice in nursing & healthcare. A guide to best practice. Philadelphia: Wolters Kluwer Health. 2019. [Google Scholar]
  • 19.Dolezel J, Zelenikova R, Finotto S, Mecugni D, Patelarou A, Panczyk M. Core Evidence-Based Practice Competencies and Learning Outcomes for European Nurses: Consensus Statements. Worldviews Evid Based Nurs. 2021;18(3):226–33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Furuki H, Sonoda N, Morimoto A. Factors related to the knowledge and skills of evidence-based practice among nurses worldwide: A scoping review. Worldviews Evid Based Nurs. 2023;20(1):16–26. doi: 10.1111/wvn.12623 [DOI] [PubMed] [Google Scholar]
  • 21.Koota E, Kääriäinen M, Kyngäs H, Lääperi M, Melender H-L. Effectiveness of Evidence-Based Practice (EBP) Education on Emergency Nurses’ EBP Attitudes, Knowledge, Self-Efficacy, Skills, and Behavior: A Randomized Controlled Trial. Worldviews Evid Based Nurs. 2021;18(1):23–32. doi: 10.1111/wvn.12485 [DOI] [PubMed] [Google Scholar]
  • 22.Bakr A, Mukhtar H. The impact of an evidence-based practice (EBP) educational program on the nursing managers’ professional knowledge/skills, attitude, and practice: Quasi-experimental study. Int J Nurs Health Care Res. 2020;3:1181. [Google Scholar]
  • 23.Gorsuch CRPF, Gallagher Ford L, Koshy Thomas B, Melnyk BM, Connor L. Impact of a Formal Educational Skill-Building Program Based on the ARCC Model to Enhance Evidence-Based Practice Competency in Nurse Teams. Worldviews Evid Based Nurs. 2020;17(4):258–68. doi: 10.1111/wvn.12463 [DOI] [PubMed] [Google Scholar]
  • 24.Yurdagül G. Examining the competence status of nurses in evidence-based practices and the influencing factors: a multicenter study in Turkey. BMC Nurs. 2025;24(1):1064. doi: 10.1186/s12912-025-03732-3 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Ylimäki S, Oikarinen A, Kääriäinen M, Pölkki T, Mikkonen K, Holopainen A, et al. Advanced practice nurses’ evidence-based healthcare competence and associated factors: A systematic review. J Clin Nurs. 2024;33(6):2069–83. doi: 10.1111/jocn.17075 [DOI] [PubMed] [Google Scholar]
  • 26.Howard B, Diug B, Ilic D. Methods of teaching evidence-based practice: a systematic review. BMC Med Educ. 2022;22(1):742. doi: 10.1186/s12909-022-03812-x [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Dabak Z, Toqan D, Malak MZ, Al-Amer R, Ayed A. Knowledge, attitudes, practice, and perceived barriers toward evidence-based practice among Palestinian nurses in intensive care units. BMC Nurs. 2024;23(1):950. doi: 10.1186/s12912-024-02646-w [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Mohammadi MM, Poursaberi R, Salahshoor MR. Evaluating the adoption of evidence-based practice using Rogers’s diffusion of innovation theory: a model testing study. Health Promot Perspect. 2018;8(1):25–32. doi: 10.15171/hpp.2018.03 [DOI] [PMC free article] [PubMed] [Google Scholar]

Decision Letter 0

Rohit Ravi

16 Feb 2026

Dear Dr. Mehammed-Ameen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Apr 02 2026 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

  • A letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols....

We look forward to receiving your revised manuscript.

Kind regards,

Rohit Ravi, Ph.D.

Academic Editor

PLOS One

Journal requirements:

When submitting your revision, we need you to address these additional requirements.

1. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

2. In the ethics statement in the Methods, you have specified that verbal consent was obtained. Please provide additional details regarding how this consent was documented and witnessed, and state whether this was approved by the IRB.

If the reviewer comments include a recommendation to cite specific previously published works, please review and evaluate these publications to determine whether they are relevant and should be cited. There is no requirement to cite these works unless the editor has indicated otherwise.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

Reviewer #1: Yes

**********

2. Has the statistical analysis been performed appropriately and rigorously? -->?>

Reviewer #1: Yes

**********

3. Have the authors made all data underlying the findings in their manuscript fully available??>

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.-->

Reviewer #1: No

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English??>

Reviewer #1: Yes

**********

Reviewer #1: This manuscript addresses an important topic and contributes context-specific evidence on evidence-based practice education among nurses in Iraq, an under-researched setting. The study design is generally appropriate, and the analyses indicate clear short-term improvements in EBP knowledge, attitudes, and self-reported practices following the intervention.

However, several issues require substantial revision before the manuscript can be considered for publication. These include clearer acknowledgment of the limitations inherent in the quasi-experimental design and non-randomized hospital allocation, greater transparency regarding the adaptation and validity of the measurement instruments, and more cautious interpretation of the unusually large effect sizes reported. In addition, the reliance on self-reported practice outcomes should be emphasized as a limitation, and conclusions should be framed accordingly.

The language is generally clear and understandable, though minor grammatical and stylistic revisions are recommended.

With these revisions, the study has the potential to make a useful contribution to the literature on EBP education in resource-constrained healthcare settings.

**********

what does this mean?). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our Privacy Policy..-->

Reviewer #1: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

To ensure your figures meet our technical requirements, please review our figure guidelines: https://journals.plos.org/plosone/s/figures

You may also use PLOS’s free figure tool, NAAS, to help you prepare publication quality figures: https://journals.plos.org/plosone/s/figures#loc-tools-for-figure-preparation.

NAAS will assess whether your figures meet our technical requirements by comparing each figure against our figure specifications.

Attachment

Submitted filename: Review Report.pdf

pone.0347252.s002.pdf (161.7KB, pdf)
PLoS One. 2026 Apr 15;21(4):e0347252. doi: 10.1371/journal.pone.0347252.r002

Author response to Decision Letter 1


23 Feb 2026

Response to Reviewers

Editor comments:

1. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming.

Response:

We thank the editor for this important reminder. We have carefully reviewed the entire submission to ensure full compliance with PLOS ONE’s style and formatting requirements.

Specifically:

• All manuscript files have been reformatted according to PLOS ONE guidelines.

2. In the ethics statement in the Methods, you have specified that verbal consent was obtained. Please provide additional details regarding how this consent was documented and witnessed, and state whether this was approved by the IRB.

Response:

We thank the editor for this important clarification. The Ethics Statement in the Methods section has been revised to provide additional details regarding the verbal consent procedure, its documentation, and IRB approval (Page 10, Line 224-232).

------------------------------------------------------------

Reviewers’ comments:

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

Reviewer #1: Yes

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

3. Have the authors made all data underlying the findings in their manuscript fully available?

Reviewer #1: No

Response: We thank the reviewer for this comment. The complete de-identified dataset underlying all findings, including the individual-level data supporting the analyses, has now been provided as Supporting Information (S1 Dataset). The Data Availability Statement has been revised accordingly to ensure full compliance with PLOS ONE policy.

4. Is the manuscript presented in an intelligible fashion and written in standard English?

Reviewer #1: Yes

5. Review Comments to the Author

Reviewer #1: This manuscript addresses an important topic and contributes context-specific evidence on evidence-based practice education among nurses in Iraq, an under-researched setting. The study design is generally appropriate, and the analyses indicate clear short-term improvements in EBP knowledge, attitudes, and self-reported practices following the intervention.

However, several issues require substantial revision before the manuscript can be considered for publication. These include clearer acknowledgment of the limitations inherent in the quasi-experimental design and non-randomized hospital allocation, greater transparency regarding the adaptation and validity of the measurement instruments, and more cautious interpretation of the unusually large effect sizes reported. In addition, the reliance on self-reported practice outcomes should be emphasized as a limitation, and conclusions should be framed accordingly.

The language is generally clear and understandable, though minor grammatical and stylistic revisions are recommended.

With these revisions, the study has the potential to make a useful contribution to the literature on EBP education in resource-constrained healthcare settings.

Response:

• We sincerely thank the reviewer for the thoughtful and constructive evaluation of our manuscript.

• We greatly appreciate the recognition of the study’s relevance and its contribution to evidence-based practice (EBP) education in an under-researched context. In response to the reviewer’s recommendations, we have undertaken substantial revisions to strengthen the manuscript.

• The Conclusion section has been revised to adopt a more cautious interpretation of the findings (Page 19-20, Line 391-400).

• The remaining recommendations are addressed in detail below in response to the major and minor comments outlined in the attached file.

6. Do you want your identity to be public for this peer review?

Reviewer #1: No

Major Comments

1. Study Design and Internal Validity

• The quasi-experimental design with hospital-level group allocation is appropriate given contextual constraints; however, non-random assignment of hospitals introduces a substantial risk of selection bias and contamination.

• Although baseline characteristics are statistically comparable, hospital-level differences (organizational culture, leadership, workload) may still explain part of the observed effects.

Recommendation: The authors should

• Explicitly acknowledge cluster-level confounding as a limitation.

• Clarify whether any steps were taken to minimize contamination (e.g., staff movement, informal knowledge sharing between hospitals).

Response:

• We agree that hospital-level allocation without randomization may introduce residual cluster-level confounding and limit internal validity. Accordingly, we have revised the Limitations section to explicitly acknowledge the potential influence of unmeasured institutional factors, which may have partially contributed to the observed effects (Page 19, Line 374-380).

• To minimize contamination, group allocation was conducted at the hospital level to prevent interaction between intervention and control participants within the same institution. The participating hospitals operate under separate administrative structures, and we assured that no formal staff rotation occurred between hospitals during the study period. Additionally, the educational intervention on the intervention group and training materials were delivered exclusively in the college of nursing /university of Kirkuk and were not accessible to control hospitals during the study period. These procedural details have now been clarified in the data collection and study instruments subsection of the methods section (Page 7, Line 147-155).

2. Sampling Strategy and Generalizability

• Convenience sampling was used, and only bachelor-prepared nurses were included.

• The sample is limited to four governmental hospitals in one city.

Recommendation: The authors should strengthen the limitations section by clearly stating that:

• Findings may not generalize to diploma nurses, private hospitals, or other regions of Iraq.

• Volunteer bias may have inflated intervention effects due to higher motivation among participants.

Response:

• We thank the reviewer for this important observation. We agree that the use of convenience sampling and restriction to bachelor-prepared nurses in four governmental hospitals may limit external validity. Accordingly, we have strengthened the Limitations section to explicitly state that the findings may not be generalizable to diploma nurses, nurses working in private-sector hospitals, or healthcare institutions in other regions of Iraq (Page 19, Line 382-386).

• We have also clarified that volunteer bias may have influenced the results, as nurses who agreed to participate may have been more motivated toward evidence-based practice, potentially inflating the observed intervention effects. These points have now been clearly incorporated into the limitations in the revised manuscript (Page 19, Line 383-386).

3. Statistical Results and Effect Sizes

• The reported effect sizes are exceptionally large (e.g., η² = 0.85 for knowledge).

• Such magnitudes are uncommon in educational interventions and raise concerns about:

Measurement sensitivity, Response bias and Ceiling effects

Recommendation: The authors should:

• Discuss why such large effects were observed.

• Acknowledge the possibility of social desirability bias, especially in self-reported practice scores.

• Consider adding confidence intervals for effect sizes or interpreting them more cautiously.

Response:

• We thank the reviewer for this important and insightful comment. We agree that the observed effect size for knowledge (η² = 0.85) is high and warrants careful interpretation.

• In the revised Discussion, we have provided a more cautious interpretation of the effect magnitude. We explain that the large effect may partially reflect low baseline knowledge levels, the structured and theory-based nature of the intervention, and the close alignment between the intervention content and the outcome measure, which may have increased sensitivity to change (Page 18, Line 349-357).

• We have also explicitly acknowledged in the Discussion section that the reliance on self-reported measures particularly for practice outcomes may have contributed to inflation of effect estimates (Page 18, Line 358-361).

4. Self- Reporting Bias

• The "Practice" domain relies on a self-administered questionnaire. Nurses may over-report

"always" performing EBP steps due to social desirability. This is a significant limitation.

Recommendation:

The discussion should clearly state that:

• Improvements in practice reflect perceived or reported behavior, not verified clinical behavior.

• Future research should incorporate audits, observations, or patient-level outcomes.

Response:

• In the discussion of the revised manuscript, we have explicitly clarified that the observed improvements in practice reflect perceived or self-reported behavior rather than objectively verified clinical performance (Page 18, Line 358-361).

• Furthermore, we have added a recommendation that future research incorporate objective evaluation methods such as clinical audits, structured observations, or patient-level outcome measures to more accurately assess the implementation of evidence-based practice (Page 18, Line 361-363).

Minor Comments

1. Abstract

• Consider adding the study design (quasi-experimental) explicitly in the first line.

• Clarify that practices are self-reported.

Response:

• We thank the reviewer for this helpful suggestion. The study design (quasi-experimental with a control group and pre–post assessments) was already specified in the Methods subsection of the Abstract. Nevertheless, we have carefully reviewed the Abstract to ensure that the design is clearly and prominently stated.

• In addition, we have revised the Abstract to explicitly clarify that the Practice domain was assessed using self-reported measures (Page 2, Line 34).

2. Introduction

• While the gap in Iraq is noted, the authors should briefly specify if there are any national nursing policies in Iraq that mandate EBP, which would further strengthen the “need” of this study.

Response:

We thank the reviewer for this constructive suggestion. In response, we have revised the Introduction to clarify the current national policy context in Iraq. Specifically, we now state that although there is increasing institutional commitment to evidence-based standards and clinical protocols, there is no explicit national policy formally mandating nurses to implement evidence-based practice as a statutory requirement. We have also highlighted the limited integration of EBP content within undergraduate nursing curricula, which may contribute to restricted implementation in practice.

This addition strengthens the contextual justification for the study and further supports the need for structured educational interventions to enhance EBP competencies among hospital nurses in Iraq (Page 4, Line 72-78).

3. Methods

• Specify whether the educational sessions were delivered by the same instructor.

• Clarify attendance thresholds (e.g., minimum sessions required for inclusion)

Response:

• We thank the reviewer for this important clarification. The Methods section has been revised to provide additional details regarding the delivery of the educational intervention and participant attendance requirements. The program was designed and delivered by one professor and two assistant professors from the College of Nursing, University of Kirkuk, all with expertise in evidence-based practice and nursing research (Page 8-9, Line 188-190).

• We have also clarified that participants were required to attend a minimum of nine out of ten sessions to be included in the intervention analysis, and attendance was recorded for each session (Page 9, Line 193-195).

4. Ethics

• Verbal consent is acceptable but briefly justify why written consent was not obtained

Response:

We thank the reviewer for this important comment. The Ethics section has been revised to clarify that verbal informed consent was used due to the minimal-risk nature of the study and because no personal identifiers were collected. The Research Ethics Committee explicitly reviewed and approved the use of verbal consent. Written consent was not deemed necessary, as participation involved completion of anonymous questionnaires and attendance at an educational program, posing no foreseeable physical or psychological risk. This clarification has been incorporated into the revised Methods section (Page 10, Line 224-230).

Attachment

Submitted filename: Response to Reviewers.docx

pone.0347252.s003.docx (25KB, docx)

Decision Letter 1

Rohit Ravi

18 Mar 2026

Dear Dr. Mehammed-Ameen,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by May 02 2026 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

  • A letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols....

We look forward to receiving your revised manuscript.

Kind regards,

Rohit Ravi, Ph.D.

Academic Editor

PLOS One

Journal Requirements:

1. If the reviewer comments include a recommendation to cite specific previously published works, please review and evaluate these publications to determine whether they are relevant and should be cited. There is no requirement to cite these works unless the editor has indicated otherwise.

2. Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

Reviewer #1: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions??>

Reviewer #1: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously? -->?>

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available??>

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.-->

Reviewer #1: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English??>

Reviewer #1: Yes

**********

Reviewer #1: (No Response)

**********

what does this mean?). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our For information about this choice, including consent withdrawal, please see our Privacy Policy..-->

Reviewer #1: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

To ensure your figures meet our technical requirements, please review our figure guidelines: https://journals.plos.org/plosone/s/figures

You may also use PLOS’s free figure tool, NAAS, to help you prepare publication quality figures: https://journals.plos.org/plosone/s/figures#loc-tools-for-figure-preparation.

NAAS will assess whether your figures meet our technical requirements by comparing each figure against our figure specifications.

Attachment

Submitted filename: Reviewer Comments to the Author.pdf

pone.0347252.s004.pdf (121.5KB, pdf)
PLoS One. 2026 Apr 15;21(4):e0347252. doi: 10.1371/journal.pone.0347252.r004

Author response to Decision Letter 2


27 Mar 2026

Response to Reviewers

Journal Requirements:

1. We note that there is identifying data in the Supporting Information file <S1_Dataset.xlsx>. Prior to sharing human research participant data, authors should consult with an ethics committee to ensure data are shared in accordance with participant consent and all applicable local laws.

Please remove or anonymize all personal information (age), ensure that the data shared are in accordance with participant consent, and re-upload a fully anonymized data set. Please note that spreadsheet columns with personal information must be removed and not hidden as all hidden columns will appear in the published file.

Response:

Thank you for your comment. All identifiable data have been carefully reviewed and removed from the dataset. Any variables that could directly or indirectly identify participants have been deleted or replaced with non-identifiable sequential numbers. Specifically, the age column was removed, and years of experience were converted into categorical variables to minimize the risk of participant identification while preserving data utility.

The revised dataset is now fully anonymized and complies with ethical standards and data-sharing policies.

2. If the reviewer comments include a recommendation to cite specific previously published works, please review and evaluate these publications to determine whether they are relevant and should be cited. There is no requirement to cite these works unless the editor has indicated otherwise.

Response:

We confirm that the reviewer did not recommend citing any specific previously published works.

3. Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Response:

We have carefully reviewed the entire reference list to ensure its completeness and accuracy. All cited articles were checked for retraction status, and none were found to be retracted. Therefore, no changes to the reference list were necessary in this regard.

Reviewer comments:

Major Comments

1. Interpretation of Effect Sizes

The authors have provided a reasonable explanation for the large effect sizes observed, particularly for knowledge (η² = 0.85). However, the interpretation could be further strengthened by adopting a slightly more cautious tone.

It is recommended to explicitly acknowledge that such unusually large effect sizes may also reflect factors such as measurement alignment with the intervention, limited variability, and potential response bias, rather than purely the magnitude of the intervention effect. Adding one or two sentences of evidence that emphasize this caution would improve interpretative rigor.

Response:

We thank the reviewer for this suggestion. We have revised the Discussion to note that the large effect size for knowledge (η² = 0.85) should be interpreted with caution, as it may reflect not only the intervention but also low baseline knowledge, close alignment of the outcome measure, quasi-experimental design, cluster-level influences, reliance on self-reported measures, potential response bias, and limited within-group variability.

Minor Comments

1. Language and Style

• Minor grammatical refinement is recommended (e.g., replace “At the other hand” with “On the other hand”).

A few sentences in the Discussion section could be shortened for clarity.

Response:

• We appreciate the reviewer’s attention to language and style. The manuscript has been carefully revised to correct minor grammatical issues throughout the manuscript.

• We thank the reviewer for this suggestion. The Discussion section has been carefully revised to shorten several long sentences for improved clarity and readability, while ensuring that all key information and interpretations are fully retained (Page 17-18, Line 310-311; 314).

2. Clarity in Discussion

• Some sections of the Discussion are slightly dense; light editing for readability would enhance overall flow.

Response:

We appreciate the reviewer’s feedback. The Discussion sections and limitation has been carefully edited to improve readability and flow. Long or dense sentences have been simplified, and paragraphs have been adjusted for clarity while ensuring that all key findings and interpretations are fully preserved (Pages 17-19, Lines 328-335; 344-351; 367-379).

Attachment

Submitted filename: Response_to_Reviewers_auresp_2.docx

pone.0347252.s005.docx (19.6KB, docx)

Decision Letter 2

Rohit Ravi

30 Mar 2026

Effect of an educational program on the knowledge, attitudes, and practices of hospital nurses regarding evidence-based practice: A quasi-experimental study

PONE-D-26-00727R2

Dear Dr. Mehammed-Ameen,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Rohit Ravi, Ph.D.

Academic Editor

PLOS One

Additional Editor Comments (optional):

The revision is satisfactory.

Reviewers' comments:

Acceptance letter

Rohit Ravi

PONE-D-26-00727R2

PLOS One

Dear Dr. Mehammed-Ameen,

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS One. Congratulations! Your manuscript is now being handed over to our production team.

At this stage, our production department will prepare your paper for publication. This includes ensuring the following:

* All references, tables, and figures are properly cited

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Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Dr. Rohit Ravi

Academic Editor

PLOS One

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    S1 Data. De-identified dataset underlying the findings of this study.

    (XLSX)

    pone.0347252.s001.xlsx (48.3KB, xlsx)
    Attachment

    Submitted filename: Review Report.pdf

    pone.0347252.s002.pdf (161.7KB, pdf)
    Attachment

    Submitted filename: Response to Reviewers.docx

    pone.0347252.s003.docx (25KB, docx)
    Attachment

    Submitted filename: Reviewer Comments to the Author.pdf

    pone.0347252.s004.pdf (121.5KB, pdf)
    Attachment

    Submitted filename: Response_to_Reviewers_auresp_2.docx

    pone.0347252.s005.docx (19.6KB, docx)

    Data Availability Statement

    All relevant data are within the paper and its Supporting Information files.


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