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. 1976 Jul;259(1):47–61. doi: 10.1113/jphysiol.1976.sp011454

The role of cyclic 3',5'-adenosine monophosphate (cyclic AMP) in the ability of sympathetic nerve stimulation to enhance growth and secretion in rat salivary glands in vivo.

T C Muir, D Templeton
PMCID: PMC1309014  PMID: 182961

Abstract

1. The cyclic AMP content of the rat salivary glands was measured after sympathetic and parasympathetic nerve stimulation, and after the administration of isoprenaline and phenylephrine. 2. Stimulation of the sympathetic nerves (20 Hz, 1 msec supramaximal voltage for 30 sec of every min) initially raised the cyclic AMP level in both the parotid and submaxillary glands. The rise, which was blocked by propranolol, was not maintained and declined during stimulation from the maximum (reached after 3 min stimulation) to control levels after approximately 15 min. Stimulation of the parasympathetic nerves for 5 min did not raise the cyclic AMP levels. 3. Isoprenaline and phenylephrine raised the cyclic AMP level in both glands. 4. Theophylline enhanced the growth of the parotid and submaxillary glands as measured either by an increase in the wet and dry weights or in acinar axes lengths. N6O2-dibutyryl cyclic monophosphoric acid (dibutyryl cyclic AMP), did not enhance the growth of the glands. 5. It is concluded that cyclic AMP does not directly control cell growth in the rat salivary glands but may be a 'trigger' for events leading eventually to increased growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almgren O. Functional significance of synthesis of noradrenaline in adrenergic nerves of rat salivary glands. J Pharm Pharmacol. 1974 Jan;26(1):23–29. doi: 10.1111/j.2042-7158.1974.tb12814.x. [DOI] [PubMed] [Google Scholar]
  2. BUTCHER R. W., SUTHERLAND E. W. Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine. J Biol Chem. 1962 Apr;237:1244–1250. [PubMed] [Google Scholar]
  3. Brenner G. M., Stanton H. C. Adrenergic mechanism responsible for submandibular salivary glandular hypertrophy in the rat. J Pharmacol Exp Ther. 1970 May;173(1):166–175. [PubMed] [Google Scholar]
  4. Carlsö B., Danielsson A., Marklund S., Stigbrand T. Effects of 3', 5'-cyclic adenosine monophosphate, 5-hydroxytryptamine, noradrenaline and theophylline on the simultaneous release of peroxidase and amylase from the guinea pig submanibular gland. Acta Physiol Scand. 1974 Jun;91(2):203–210. doi: 10.1111/j.1748-1716.1974.tb05677.x. [DOI] [PubMed] [Google Scholar]
  5. Delfs U., Emmelin N. Degeneration secretion of saliva in the rat following sympathectomy. J Physiol. 1974 Jun;239(3):623–630. doi: 10.1113/jphysiol.1974.sp010585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Durham J. P., Baserga R., Butcher F. R. The effect of isoproterenol and its analogs upon adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate levels in mouse parotid gland in vivo. Relationship to the stimulation of DNA synthesis. Biochim Biophys Acta. 1974 Nov 4;372(1):196–217. doi: 10.1016/0304-4165(74)90087-7. [DOI] [PubMed] [Google Scholar]
  7. Guidotti A., Weiss B., Costa E. Adenosine 3',5'-monophosphate concentrations and isoproterenol-induced synthesis of deoxyribonucleic acid in mouse parotid gland. Mol Pharmacol. 1972 Sep;8(5):521–530. [PubMed] [Google Scholar]
  8. Henion W. F., Sutherland E. W., Posternak T. Effects of derivatives of adenosine 3',5'-phosphate on liver slices and intact animals. Biochim Biophys Acta. 1967 Oct 9;148(1):106–113. doi: 10.1016/0304-4165(67)90284-x. [DOI] [PubMed] [Google Scholar]
  9. Jaanus S. D., Rubin R. P. Analysis of the role of cyclic adenosine 3',5'-monophosphate in catecholamine release. J Physiol. 1974 Mar;237(2):465–476. doi: 10.1113/jphysiol.1974.sp010492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Ledda F., Marchetti P., Mugelli A. Studies on the positive inotropic effect of phenylephrine: a comparison with isoprenaline. Br J Pharmacol. 1975 May;54(1):83–90. doi: 10.1111/j.1476-5381.1975.tb07413.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Malamud D. Amylase secretion from mouse parotid and pancreas: role of cyclic AMP and isoproterenol. Biochim Biophys Acta. 1972 Sep 15;279(2):373–376. doi: 10.1016/0304-4165(72)90155-9. [DOI] [PubMed] [Google Scholar]
  13. Muir T. C., Pollock D., Turner C. J. The effects of electrical stimulation of the autonomic nerves and of drugs on the size of salivary glands and their rate of cell division. J Pharmacol Exp Ther. 1975 Nov;195(2):372–381. [PubMed] [Google Scholar]
  14. Muir T. C., Templeton D. The role of adenosine 3',5'-monophosphate (cyclic AMP) in the catecholamine-induced growth of rat salivary glands. J Physiol. 1975 Mar;246(2):85P–86P. [PubMed] [Google Scholar]
  15. Norberg K. A., Olson L. Adrenergic innervation of the salivary glands in the rat. Z Zellforsch Mikrosk Anat. 1965 Oct 12;68(2):183–189. doi: 10.1007/BF00342427. [DOI] [PubMed] [Google Scholar]
  16. SELYE H., VEILLEUX R., CANTIN M. Excessive stimulation of salivary gland growth by isoproterenol. Science. 1961 Jan 6;133(3445):44–45. doi: 10.1126/science.133.3445.44. [DOI] [PubMed] [Google Scholar]
  17. Schneyer C. A., Hall H. D. Autonomic pathways involved in a sympathetic-like action of pilocarpine on salivary composition. Proc Soc Exp Biol Med. 1966 Jan;121(1):96–100. doi: 10.3181/00379727-121-30707. [DOI] [PubMed] [Google Scholar]
  18. Schneyer L. H., Young J. A., Schneyer C. A. Salivary secretion of electrolytes. Physiol Rev. 1972 Jul;52(3):720–777. doi: 10.1152/physrev.1972.52.3.720. [DOI] [PubMed] [Google Scholar]
  19. Wooten G. F., Thoa N. B., Kopin I. J., Axelrod J. Enhanced release of dopamine -hydroxylase and norepinephrine from sympathetic nerves by dibutyryl cyclic adenosine 3', 5'-monophosphate and theophylline. Mol Pharmacol. 1973 Mar;9(2):178–183. [PubMed] [Google Scholar]

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