Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1976 Oct;261(2):337–357. doi: 10.1113/jphysiol.1976.sp011562

The secretory characteristics of dehydrocholate in the dog: comparison with the natural bile salts.

E R O'Máille, T G Richards
PMCID: PMC1309145  PMID: 978576

Abstract

1. During dehydrocholate administration in the taurine replete dog, the maximum excretory rate of total bile salt (almost entirely dehydrocholate derivative, mostly conjugated) was 3-84 +/- 0-53 (S.D.) mumole/min. kg body wt. (eleven experiments). This was much less than the excretory maximum previously obtained for taurocholate (8-64 +/- 1-31 (S.D.) mumole/min. kg total cholate, mostly conjugated). 2. The superimposition of taurocholate infusion did not cause any significant change in the 'dehydrocholate' maximum but taurocholate itself was excreted into bile at no more than about half its normal maximum. When taurocholate maximum excretion was established first, it was reduced by dehydrocholate administration. In both types of experiment the joint bile salt excretory maximum was of the same order as that of taurocholate alone, provided taurocholate made up at least 40-50% of the total bile salt. 3. When taurocholate administration was stopped, the maximum excretory rate of 'dehydrocholate' rose to values up to 63% above the initially determined excretory maximum; the enhanced 'dehydrocholate' excretory maximum, when calculated for optimal conditions, approached that of actively conjugated vholate, even though the effective 'dehydrocholate' concentration in bile was ten to twenty times the critical micellar concentration of taurocholate. This suggests that the effective bile salt concentration in bile is not an important determinant of the secretory performance of a bile salt. 4. To explain findings (2) and (3) it is necessary to postulate that taurocholate has both a facilitatory and an inhibitory action on 'dehydrocholate' excretion. The facilitatory action, which persists after taurocholate has left the animal, may consist either of an increase in the maximum rate at which modification of dehydrocholate takes place within the liver cell, or an increase in the number of functioning 'carriers' for 'dehydrocholate' transfer. The data suggest that the inhibitory effect is due to the competitive interaction that also appears to exist between the two bile salts. 5. The increase in bile flow rate per unit increase in 'dehydrocholate' excretion (15 ml./m-mole) was about twice that obtained for taurocholate. There was no significant formation of micellar aggregates during 'dehydrocholate' excretion, as judged from the total electrolyte concentration of bile and its osmalality. 6. During the excretion of 'dehydrocholate'-taurocholate mixtures (approximately 1:1) at submaximal rates the associated bile flow rate was not less than the sum of the separate components, thus suggesting that 'dehydrocholate' was not being incorporated in taurocholate mixed micelles.

Full text

PDF
337

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIZARD G., VANLERENBERGHE J. Cholérèse et cholérétiques. J Physiol (Paris) 1956 Mar-Apr;48(2):207–364. [PubMed] [Google Scholar]
  2. Desjeux J. F., Erlinger S., Dumont M. Mètabolisme et influence sur la sècrètion biliaire du dèhydrocholate chez le chien. Biol Gastroenterol (Paris) 1973 Jan-Feb;6(1):9–18. [PubMed] [Google Scholar]
  3. O'Máille E. R., Richards T. G., Short A. H. Acute taurine depletion and maximal rates of hepatic conjugation and secretion of cholic acid in the dog. J Physiol. 1965 Sep;180(1):67–79. [PMC free article] [PubMed] [Google Scholar]
  4. O'Máille E. R., Richards T. G., Short A. H. Factors determining the maximal rate of organic anion secretion by the liver and further evidence on the hepatic site of action of the hormone secretin. J Physiol. 1966 Oct;186(2):424–438. doi: 10.1113/jphysiol.1966.sp008044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. O'Máille E. R., Richards T. G., Short A. H. Observations on the elimination rates of single injections of taurocholate and cholate in the dog. Q J Exp Physiol Cogn Med Sci. 1969 Jul;54(3):296–310. doi: 10.1113/expphysiol.1969.sp002028. [DOI] [PubMed] [Google Scholar]
  6. O'Máille E. R., Richards T. G., Short A. H. The influence of conjugation of cholic acid on its uptake and secretion: hepatic extraction of taurocholate and cholate in the dog. J Physiol. 1967 Apr;189(2):337–350. doi: 10.1113/jphysiol.1967.sp008172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rutishauser S. C., Stone S. L. Comparative effects of sodium taurodeoxycholate and sodium taurocholate on bile secretion in the rat, dog and rabbit. J Physiol. 1975 Mar;245(3):583–598. doi: 10.1113/jphysiol.1975.sp010863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SPERBER I. Secretion of organic anions in the formation of urine and bile. Pharmacol Rev. 1959 Mar;11(1):109–134. [PubMed] [Google Scholar]
  9. Soloway R. D., Hofmann A. F., Thomas P. J., Schoenfield L. J., Klein P. D. Triketocholanoic (dehydrocholic) acid. Hepatic metabolism and effect on bile flow and biliary lipid secretion in man. J Clin Invest. 1973 Mar;52(3):715–724. doi: 10.1172/JCI107233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wheeler H. O., King K. K. Biliary excretion of lecithin and cholesterol in the dog. J Clin Invest. 1972 Jun;51(6):1337–1350. doi: 10.1172/JCI106930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wheeler H. O., Ramos O. L. DETERMINANTS OF THE FLOW AND COMPOSITION OF BILE IN THE UNANESTHETIZED DOG DURING CONSTANT INFUSIONS OF SODIUM TAUROCHOLATE. J Clin Invest. 1960 Jan;39(1):161–170. doi: 10.1172/JCI104015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Young D. L., Hanson K. C. Effect of bile salts on hepatic phosphatidylcholine synthesis and transport into rat bile. J Lipid Res. 1972 Mar;13(2):244–252. [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES