Skip to main content
The Journal of Physiology logoLink to The Journal of Physiology
. 1976 Jan;254(1):39–54. doi: 10.1113/jphysiol.1976.sp011219

An analysis of the action of cations of the lanthanide series on the mechanical responses of guinea-pig ileal longitudinal muscle.

C R Triggle, D J Triggle
PMCID: PMC1309178  PMID: 1249741

Abstract

1. The inhibitory effects of lanthanide cations (Ln3+) on mechanical responses and 45Ca uptake in guinea-pig ileal longitudinal smooth muscle were studied. 2. Ln3+ strongly inhibited the phasic and tonic component of the response to the muscarinic agonist cis-2-methyl-4-dimethylaminomethyl-1,3-dioxolane methiodide (CD) the two components being affected to the same extent. Inhibition was also obtained for the responses evoked by high K+ but here the effect was mainly on the phasic response, the tonic component was merely delayed. 3. Other members of the Ln3+ series, with the exception of cerium, were found to be more effective than lanthanum in their ability to inhibit the CD response. Thulium, Tm3+, the thirteenth member of the series was the most effective cation. 4. Analysis of 170Tm uptake revealed at least two components. The concentration-dependence of one component, saturating at 2-5 x 10(-6) Tm, corresponded closely to that of the inhibitory effect of Tm3+ on contraction. 5. 170Tm uptake as a function of time showed a secondary rise after 30 min of exposure to the lanthanide. 6. Although 2-5 x 10(-6) M-Tm3+ produced 90% inhibition of the CD and the high K+ induced responses significant reduction of 45Ca uptake by the muscle was only detected when much higher Tm3+ concentrations (greater than or equal 10(-3) M-Tm3+) were used. 7. It is concluded that Ln3+ combine with membrane sites specifically involved in Ca2+ translocation during excitation-contraction coupling.

Full text

PDF
39

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOHR D. F. ELECTROLYTES AND SMOOTH MUSCLE CONTRACTION. Pharmacol Rev. 1964 Mar;16:85–127. [PubMed] [Google Scholar]
  2. Bannister L. H. Lanthanum as an intracellular stain. J Microsc. 1972 Jun;95(3):413–419. doi: 10.1111/j.1365-2818.1972.tb01042.x. [DOI] [PubMed] [Google Scholar]
  3. Barton P. G. The influence of surface charge density of phosphatides on the binding of some cations. J Biol Chem. 1968 Jul 25;243(14):3884–3890. [PubMed] [Google Scholar]
  4. Bloom S., Brady A. J., Langer G. A. Calcium metabolism and active tension in mechanically disaggregated heart muscle. J Mol Cell Cardiol. 1974 Apr;6(2):137–147. doi: 10.1016/0022-2828(74)90017-0. [DOI] [PubMed] [Google Scholar]
  5. Chang K. J., Triggle D. J. Quantitative aspects of drug-receptor interactions. I. Ca2+ and cholinergic receptor activation in smooth muscle: a basic model for drug-receptor interactions. J Theor Biol. 1973 Jul;40(1):125–154. doi: 10.1016/0022-5193(73)90168-9. [DOI] [PubMed] [Google Scholar]
  6. Diamond J. M., Wright E. M. Biological membranes: the physical basis of ion and nonelectrolyte selectivity. Annu Rev Physiol. 1969;31:581–646. doi: 10.1146/annurev.ph.31.030169.003053. [DOI] [PubMed] [Google Scholar]
  7. Fleckenstein A. Physiologie und Pharmakologie der transmembranären Natrium-, Kalium- und Calcium-Bewegungen. Arzneimittelforschung. 1972 Dec;22(12):2019–2028. [PubMed] [Google Scholar]
  8. Freeman D. J., Daniel E. E. Calcium movement in vascular smooth muscle and its detection using lanthanum as a tool. Can J Physiol Pharmacol. 1973 Dec;51(12):900–913. doi: 10.1139/y73-139. [DOI] [PubMed] [Google Scholar]
  9. Goodford P. J. The calcium content of the smooth muscle of the guinea-pig taenia coli. J Physiol. 1967 Sep;192(1):145–157. doi: 10.1113/jphysiol.1967.sp008293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goodman F. R., Weiss G. B. Dissociation by lanthanum of smooth muscle responses to potassium and acetylcholine. Am J Physiol. 1971 Mar;220(3):759–766. doi: 10.1152/ajplegacy.1971.220.3.759. [DOI] [PubMed] [Google Scholar]
  11. Hodgson B. J., Kidwai A. M., Daniel E. E. Uptake of lanthanum by smooth muscle. Can J Physiol Pharmacol. 1972 Jul;50(7):730–733. doi: 10.1139/y72-107. [DOI] [PubMed] [Google Scholar]
  12. Hurwitz L., Suria A. The link between agonist action and response in smooth muscle. Annu Rev Pharmacol. 1971;11:303–326. doi: 10.1146/annurev.pa.11.040171.001511. [DOI] [PubMed] [Google Scholar]
  13. Kohlhardt M., Bauer B., Krause H., Fleckenstein A. Differentiation of the transmembrane Na and Ca channels in mammalian cardiac fibres by the use of specific inhibitors. Pflugers Arch. 1972;335(4):309–322. doi: 10.1007/BF00586221. [DOI] [PubMed] [Google Scholar]
  14. Langer G. A., Frank J. S. Lanthanum in heart cell culture. Effect on calcium exchange correlated with its localization. J Cell Biol. 1972 Sep;54(3):441–455. doi: 10.1083/jcb.54.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Langer G. A., Serena S. D., Nudd L. M. Cation exchange in heart cell culture: correlation with effects on contractile force. J Mol Cell Cardiol. 1974 Apr;6(2):149–161. doi: 10.1016/0022-2828(74)90018-2. [DOI] [PubMed] [Google Scholar]
  16. Lehninger A. L., Carafoli E. The interaction of La 3+ with mitochondria in relation to respiration-coupled Ca 2+ transport. Arch Biochem Biophys. 1971 Apr;143(2):506–515. doi: 10.1016/0003-9861(71)90235-9. [DOI] [PubMed] [Google Scholar]
  17. Mayer C. J., van Breemen C., Casteels T. The action of lanthanum and D600 on the calcium exchange in the smooth muscle cells of the guinea-pig Taenia coli. Pflugers Arch. 1972;337(4):333–350. doi: 10.1007/BF00586650. [DOI] [PubMed] [Google Scholar]
  18. Moore C. L. Specific inhibition of mitochondrial Ca++ transport by ruthenium red. Biochem Biophys Res Commun. 1971 Jan 22;42(2):298–305. doi: 10.1016/0006-291x(71)90102-1. [DOI] [PubMed] [Google Scholar]
  19. PATON W. D., RANG H. P. THE UPTAKE OF ATROPINE AND RELATED DRUGS BY INTESTINAL SMOOTH MUSCLE OF THE GUINEA-PIG IN RELATION TO ACETYLCHOLINE RECEPTORS. Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:1–44. doi: 10.1098/rspb.1965.0058. [DOI] [PubMed] [Google Scholar]
  20. RANG H. P. STIMULANT ACTIONS OF VOLATILE ANAESTHETICS ON SMOOTH MUSCLE. Br J Pharmacol Chemother. 1964 Apr;22:356–365. doi: 10.1111/j.1476-5381.1964.tb02040.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. ROTHSTEIN A., MEIER R. The relationship of the cell surface to metabolism. VI. The chemical nature of uranium-complexing groups of the cell surface. J Cell Physiol. 1951 Oct;38(2):245–270. doi: 10.1002/jcp.1030380209. [DOI] [PubMed] [Google Scholar]
  22. Reed K. C., Bygrave F. L. Accumulation of lanthanum by rat liver mitochondria. Biochem J. 1974 Feb;138(2):239–252. doi: 10.1042/bj1380239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sottocasa G., Sandri G., Panfili E., De Bernard B., Gazzotti P., Vasington F. D., Carafoli E. Isolation of a soluble Ca 2+ binding glycoprotein from ox liver mitochondria. Biochem Biophys Res Commun. 1972 May 26;47(4):808–813. doi: 10.1016/0006-291x(72)90564-5. [DOI] [PubMed] [Google Scholar]
  24. Triggle D. J. Adrenergic receptors. Annu Rev Pharmacol. 1972;12:185–196. doi: 10.1146/annurev.pa.12.040172.001153. [DOI] [PubMed] [Google Scholar]
  25. Van Breemen C. Blockade of membrane calcium fluxes by lanthanum in relation to vascular smooth muscle contractility. Arch Int Physiol Biochim. 1969 Oct;77(4):710–716. doi: 10.3109/13813456909059783. [DOI] [PubMed] [Google Scholar]
  26. Van Breemen C., Farinas B. R., Gerba P., McNaughton E. D. Excitation-contraction coupling in rabbit aorta studied by the lanthanum method for measuring cellular calcium influx. Circ Res. 1972 Jan;30(1):44–54. doi: 10.1161/01.res.30.1.44. [DOI] [PubMed] [Google Scholar]
  27. Van Breemen C. Permselectivity of a porous phospholipid-cholesterol artificial membrane. Calcium and lanthanum effects. Biochem Biophys Res Commun. 1968 Sep 30;32(6):977–983. doi: 10.1016/0006-291x(68)90124-1. [DOI] [PubMed] [Google Scholar]
  28. Weiss G. B., Goodman F. R. Effects of lanthanum on contraction, calcium distribution and Ca45 movements in intestinal smooth muscle. J Pharmacol Exp Ther. 1969 Sep;169(1):46–55. [PubMed] [Google Scholar]
  29. van Breemen C., De Weer P. Lanthanum inhibition of 45Ca efflux from the squid giant axon. Nature. 1970 May 23;226(5247):760–761. doi: 10.1038/226760a0. [DOI] [PubMed] [Google Scholar]
  30. van Breemen C., Farinas B. R., Casteels R., Gerba P., Wuytack F., Deth R. Factors controlling cytoplasmic Ca 2+ concentration. Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):57–71. doi: 10.1098/rstb.1973.0009. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES