Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1976 Mar;255(3):619–634. doi: 10.1113/jphysiol.1976.sp011299

Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

B Morris, R Morris
PMCID: PMC1309270  PMID: 1263138

Abstract

1. The plasma volume in rats aged 15-16 days was measured by dilution analysis using homologous, 125I-labelled immunoglobulin G. A mean plasma volume of 5-53 ml./100 g and a mean blood volume of 8-01 ml./100 g were obtained.2. After the injection of labelled immunoglobulin G into the heart, homogenates of various abdominal organs and of the carcass were prepared. Labelled immunoglobulin G left the vascular compartment at a rate of about 9-10%/hr over a 3 hr period. About 11% of the labelled immunoglobulin G was catabolized in 2 hr. 3. The data obtained from these studies was used to make quantitative estimates of the amount of intact immunoglobulin G transmitted from the proximal intestine and from the ileum after the intra-intestinal injection of 1000 mug of labelled immunoglobulin G.Homogenates of the experimental animals were prepared and it was estimated that over 40% of the labelled immunoglobulin G was transmitted as intact protein from the proximal intestine. The results suggest that no intact immunoglobulin G was transmitted from the ileum, but about 15% of the protein removed from the ileum was recovered in the whole body as degraded fragments precipitable with trichloroacetic acid. 4. These observations are discussed in the context of the transmission of antibodies, and their relevance to the receptor hypothesis is considered.

Full text

PDF
619

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN S. B., GLENERT J., WALLEVIK K. GAMMA GLOBULIN TURNOVER AND INTESTINAL DEGRADATION OF GAMMA GLOBULIN IN THE DOG. J Clin Invest. 1963 Dec;42:1873–1881. doi: 10.1172/JCI104872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BANGHAM D. R., TERRY R. J. The absorption of 131I-labelled homologous and heterologous serum proteins fed orally to young rats. Biochem J. 1957 Aug;66(4):579–583. doi: 10.1042/bj0660579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BELCHER E. H., HARRISS E. B. Studies of plasma volume, red cell volume and total blood volume in young growing rats. J Physiol. 1957 Nov 14;139(1):64–78. doi: 10.1113/jphysiol.1957.sp005875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clarke R. M., Hardy R. N. The use of [125-I] polyvinyl pyrrolidone K. 60 in the quantitative assessment of the uptake of macromolecular substances by the intestine of the young rat. J Physiol. 1969 Sep;204(1):113–125. doi: 10.1113/jphysiol.1969.sp008902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GITLIN D., KLINENBERG J. R., HUGHES W. L. Site of catabolism of serum albumin. Nature. 1958 Apr 12;181(4615):1064–1065. doi: 10.1038/1811064b0. [DOI] [PubMed] [Google Scholar]
  6. Jones E. A., Waldmann T. A. The mechanism of intestinal uptake and transcellular transport of IgG in the neonatal rat. J Clin Invest. 1972 Nov;51(11):2916–2927. doi: 10.1172/JCI107116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones R. E. Intestinal absorption and degradation of rat and bovine gamma-globulins in the suckling rat. Biochim Biophys Acta. 1972 Feb 11;255(2):530–538. doi: 10.1016/0005-2736(72)90156-3. [DOI] [PubMed] [Google Scholar]
  8. Morris B., Morris R. The absorption of 125I-labelled immunoglobulin G by different regions of the gut in young rats. J Physiol. 1974 Sep;241(3):761–770. doi: 10.1113/jphysiol.1974.sp010683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morris B. The transmission of -125-I-labelled immunoglobulin G by proximal and distal regions of the small intestine of 16-day-old rats. J Physiol. 1975 Feb;245(1):249–259. doi: 10.1113/jphysiol.1975.sp010843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rodewald R. Intestinal transport of antibodies in the newborn rat. J Cell Biol. 1973 Jul;58(1):189–211. doi: 10.1083/jcb.58.1.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rosa U., Pennisi F., Bianchi R., Federighi G., Donato L. Chemical and biological effects of iodination on human albumin. Biochim Biophys Acta. 1967 Apr 11;133(3):486–498. doi: 10.1016/0005-2795(67)90553-3. [DOI] [PubMed] [Google Scholar]
  12. TRAVNICKOVA E., HELLER J. PLASMA AND BLOOD VOLUME OF INFANT RATS DURING THE FIRST POSTNATAL MONTH. Physiol Bohemoslov. 1963;12:541–547. [PubMed] [Google Scholar]
  13. Waldmann T. A., Jones E. A. The role of cell-surface receptors in the transport and catabolism of immunoglobulins. Ciba Found Symp. 1972;9:5–23. doi: 10.1002/9780470719923.ch2. [DOI] [PubMed] [Google Scholar]
  14. Waldmann T. A., Strober W. Metabolism of immunoglobulins. Prog Allergy. 1969;13:1–110. doi: 10.1159/000385919. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES