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. 1976 Mar;256(1):209–225. doi: 10.1113/jphysiol.1976.sp011321

The preparation of human red cell ghosts containing calcium buffers.

T J Simons
PMCID: PMC1309301  PMID: 933031

Abstract

1. Ca buffers may be introduced into human red cells by reversible haemolysis. The resealed ghosts retain Ca and chelating anions in the same ratio as in the haemolysing solution, enabling the intracellular Ca2+ concentration to be calculated simply. 2. The passive permeability of the ghosts to Na and Cl is unaffected by intracellular Ca2+ concentrations in the 10(-8)-10(-4) M range, whereas the K permeability is greatly increased at concentrations above 10(-7) M. 3. These preparations enable Ca-dependent K movements to be studied under stable conditions. When the ghosts contain about 5 X 10(-6) M-Ca2+, over 96% of K transport occurs via the Ca-sensitive route.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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