There was a mistake in Figure 2 as published. While compiling original Figure 2a, inadvertently wrong images with overlapping field of view were used for NG and HG groups. Increased acetylation of Mfn2 in high glucose, as shown in Figure 2a, was further supported by increased fluorescence of acetyl lysine and Mfn2:acetyl lysine Pearson correlation coefficient, and by the co-immunoprecipitation data (original Figures 2b–e).
Figure 2.
Acetylation of Mfn2. (A) Representative immunofluorescence image showing staining of Mfn2 (Alexa Fluor-488 conjugated secondary antibody, green) and acetyl lysine (Texas red-conjugated secondary antibody). Cells were imaged under an ApoTome microscope with a 20X objective. (B) Fluorescence intensity of acetyl lysine was quantified using Zeiss software module and the data are expressed as arbitrary units (AU). (C) Pearson correlation coefficient between acetyl lysine and Mfn2 was calculated using Zeiss software module. (D) Representative image of Mfn2 western blot in acetyl lysine immunoprecipitated HRECs. (E) Intensity of the acetylated Mfn2 (Ac-Mfn2) was quantified by image J software. The values are represented as mean ± SD. NG= 5mM D-glucose, HG= 20mM D-glucose, HG/Resv = cells incubated in 20mM D-glucose in the presence of resveratrol; L-Gl= 20mM L-glucose.*p<0.05 vs NG and #p<0.05 vs HG.
The corrected Figure 2 appears below.
The original version of this article has been updated.
Footnotes
Edited and reviewed by: Guy A. Rutter, Imperial College London, United Kingdom
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