Abstract
Complement-fixation (CF) and tube agglutination (TA) tests for demonstration of Vibrio fetus antibodies were conducted on the sera of three groups of ten heifers. One group was vaccinated subcutaneously with a commercial V. fetus var venerealis bacterin and challenged intra-utero, at the external os cervicus one month later; the second was infected only and the third vaccinated only.
The vaccinated cattle developed high CF serum titers, but no such increase was observed in animals infected only. A moderate increase in serum antibody titers was demonstrated by the TA test following either infection or vaccination; although titers observed were not higher than those observed in the sera of some apparently normal uninfected animals. The group receiving both vaccine and challenge was the only one in which significant serum antibody titers were demonstrable by the TA test. The sera of these animals also had significant titers in the CF tests.
The CF and TA tests detected serum antibodies produced by the parenteral inoculation of V. fetus antigen. These two tests were of limited value in detecting serum antibodies from animals with genital V. fetus var venerealis infection, although the formation of local antibodies was demonstrable by the vaginal mucus agglutination test.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Clark B. L., Dufty J. H., Monsbourgh M. J. Experimental Vibrio fetus (venerealis) infection in heifers. The immunising properties of killed organisms injected subcutaneously. Aust Vet J. 1968 Mar;44(3):110–114. doi: 10.1111/j.1751-0813.1968.tb09034.x. [DOI] [PubMed] [Google Scholar]
- Clark B. L., Dufty J. H., Monsbourgh M. J. Vaccination of heifers with Vibrio fetus (intestinal type) against infection with Vibrio fetus (genital type). J Comp Pathol. 1970 Jan;80(1):47–52. doi: 10.1016/0021-9975(70)90030-7. [DOI] [PubMed] [Google Scholar]
- MELLICK P. W., WINTER A. J., MCENTEE K. DIAGNOSIS OF VIBRIOSIS IN THE BULL BY USE OF THE FLUORESCENT ANTIBODY TECHNIC. Cornell Vet. 1965 Apr;55:280–294. [PubMed] [Google Scholar]
- Mitchell D. Some effects of experimental Vibrio fetus (venerealis) infection on cattle inoculated with a commercial bacterin. Can J Comp Med. 1968 Jul;32(3):474–479. [PMC free article] [PubMed] [Google Scholar]
- PLASTRIDGE W. N., EASTERBROOKS H. L., WILLIAMS L. F., KIGGINS E. M., WALKER E. Reproductive efficiency and serological findings in heifers experimentally infected with Vibrio fetus. Am J Vet Res. 1955 Oct;16(61 Pt 1):493–498. [PubMed] [Google Scholar]
- Robertson A., Bannister G. L., Ruckerbauer G. M., Boulanger P. Toxoplasmosis II. Studies of Animal Sera Using Complement-Fixation Tests. Can J Comp Med Vet Sci. 1963 Jul;27(7):169–176. [PMC free article] [PubMed] [Google Scholar]
- Speck J. Die serologische Differenzierung von Hühnervibrionen mittels der Komplementbindungsreaktion. Zentralbl Veterinarmed B. 1965 Nov;12(6):541–546. [PubMed] [Google Scholar]