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. 1972 Jan;36(1):26–33.

Heterogeneity of Foot-and-mouth Disease Virus: Further Studies on Plaque Formation by Two Plaque-size Variants

J S Martinsen 1
PMCID: PMC1319651  PMID: 4334291

Abstract

Plaque production by a small-plaque (SP) and large-plaque (LP) variant of foot-and-mouth disease virus, type A, strain 119 (FMDV, A119), was influenced by a number of environmental factors. The SP variant produced plaques on cells of the IB-RS-2 cell line from swine kidney and to a lesser degree on primary cultures of swine kidney cells, but plaque formation was inhibited on primary cultures of bovine kidney (BK) cells unless diethylaminoethyl (DEAE) dextran was added to agar overlays. When DEAE dextran-treated agar overlay was used, the LP variant formed larger plaques on BK cells but not on IB-RS-2 cells. Concentrations of DEAE dextran from 0 to 100 µg/ml greatly enhanced the formation of SP virus plaques on BK cells but had little or no effect on the average size of plaques produced by the LP variant. Higher concentrations of polycation enlarged the plaques formed by both variants. Plaque sizes of the SP and LP variants increased as the concentration of agar or agarose in the overlays decreased. Reducing the concentration of agar to 0.75% facilitated the formation of SP virus plaques, but better plaque production occurred under agarose overlays.

The original parent virus consisted predominantly of virus particles that formed small plaques. The rate of neutralization of the parent virus by guinea pig antiserum prepared against the parent virus was faster than antiserum inactivation of a low-passage virus of the same serotype and strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BACHRACH H. L., CALLIS J. J., HESS W. R., PATTY R. E. A plaque assay for foot-and-mouth disease virus and kinetics of virus reproduction. Virology. 1957 Oct;4(2):224–236. doi: 10.1016/0042-6822(57)90060-0. [DOI] [PubMed] [Google Scholar]
  2. BACHRACH H. L., CALLIS J. J., HESS W. R., PATTY R. E., DE BOER C. J., HAMBLET F. E. Large-scale production of bovine kidney cultures for plaque assay of foot-and-mouth disease virus and its ribonucleic acid. Am J Vet Res. 1962 May;23:608–613. [PubMed] [Google Scholar]
  3. Cowan K. M. Immunochemical studies of foot-and-mouth disease. V. Antigenic variants of virus demonstrated by immunodiffusion analyses with 19S but not 7S antibodies. J Exp Med. 1969 Feb 1;129(2):333–350. doi: 10.1084/jem.129.2.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DINTER Z., PHILIPSON L., WESSLEN T. Properties of foot-and-mouth disease virus in tissue culture. I. Changes in plaque morphology and antigenicity following passage in tissue culture. Arch Gesamte Virusforsch. 1959;9:411–427. doi: 10.1007/BF01248831. [DOI] [PubMed] [Google Scholar]
  5. DINTER Z., SIBALIN M. Uber die Plaque-Bildung durch das Maul-und-Klauenseuche-Virus. Arch Gesamte Virusforsch. 1958;8(3):385–396. [PubMed] [Google Scholar]
  6. Dougherty E., 3rd, Seibold H. R., Patty R. E. Effects of chronic residence on size and size distribution of plaques of type A foot-and-mouth disease virus in primary calf kidney cell cultures. Am J Vet Res. 1968 Mar;29(3):693–701. [PubMed] [Google Scholar]
  7. HYDE J. L., GRAVES J. H. The comparative titration of foot-and-mouth disease virus inoculated into the tongue and foot pads of guinea pigs. Am J Vet Res. 1963 May;24:642–643. [PubMed] [Google Scholar]
  8. HYDE J. L. The use of solid carbon dioxide for producing short periods of anesthesia in guinea pigs. Am J Vet Res. 1962 May;23:684–685. [PubMed] [Google Scholar]
  9. MARTINSEN J. S. INACTIVATION OF FOOT-AND-MOUTH DISEASE VIRUS BY GLYCIDALDEHYDE. Am J Vet Res. 1964 Sep;25:1417–1423. [PubMed] [Google Scholar]
  10. Martinsen J. S. The effect of agarose overlay on plaque formation by a small-plaque-forming virus of foot-and-mouth disease. Res Vet Sci. 1970 Jul;11(4):387–388. [PubMed] [Google Scholar]
  11. Martinsen J. S. The effect of diethylaminoethyl dextran and agar overlay pH on plaque formation by two plaque-size variants of foot-and-mouth disease virus. Can J Comp Med. 1970 Jan;34(1):13–19. [PMC free article] [PubMed] [Google Scholar]
  12. POLATNICK J., BACHRACH H. L. PRODUCTION AND PURIFICATION OF MILLIGRAM AMOUNTS OF FOOT-AND-MOUTH DISEASE VIRUS FROM BABY HAMSTER KIDNEY CELL CULTURES. Appl Microbiol. 1964 Jul;12:368–373. doi: 10.1128/am.12.4.368-373.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Patty R. E. Inhibition of foot-and-mouth disease virus by normal bovine serum. Am J Vet Res. 1970 Jan;31(1):165–171. [PubMed] [Google Scholar]
  14. SEIBOLD H. R., COTTRAL G. E., PATTY R. E., GAILIUNAS P. APPARENT MODIFICATION OF FOOT-AND-MOUTH DISEASE VIRUS AFTER PROLONGED RESIDENCE IN SURVIVING CELLS. Am J Vet Res. 1964 May;25:806–814. [PubMed] [Google Scholar]
  15. SELLERS R. F., BURT L. M., CUMMING A., STEWART D. L. The behaviour of strains of the virus of foot-and-mouth disease in pig,calf,ox and lamb kidney tissue cultures. Arch Gesamte Virusforsch. 1960;9:637–646. doi: 10.1007/BF01242149. [DOI] [PubMed] [Google Scholar]

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