Abstract
Twenty-seven pigs from three litters were used in a comparison of body weights, organ weights, and selected histological features of germfree, conventional and isolator-reared contaminated pigs. At three weeks of age conventional pigs were heavier than pigs of the other two groups. The mandibular lymphnodes, stomachs, and small intestines of contaminated pigs were significantly heavier than the same organs of germfree pigs. This difference was not found in superficial inguinal or prefemoral lymph nodes. Other statistically significant organ weight differences were found.
Histologically, the lymph nodes of conventional and contaminated pigs were much more active than those of germfree pigs, although secondary nodules were occasionally found in lymph nodes of germfree pigs. Greater quantities of iron-containing pigment were found in the spleens of germfree pigs than in spleens of the other two groups. Hepatic interlobular septa were somewhat more developed in conventional pigs than in germfree or contaminated pigs.
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- Alexander T. J., Miniats O. P., Ingram D. G., Thomson R. G., Thackeray E. L. Gnotobiotic pigs: procurement, microbial flora, serum proteins and lymphatic tissues. Can Vet J. 1969 Apr;10(4):98–105. [PMC free article] [PubMed] [Google Scholar]
- BAUER H., HOROWITZ R. E., LEVENSON S. M., POPPER H. The response of the lymphatic tissue to the microbial flora. Studies on germfree mice. Am J Pathol. 1963 Apr;42:471–483. [PMC free article] [PubMed] [Google Scholar]
- Cross R. F., Kohler E. M. Autolytic changes in the digestive system of germfree, escherichia coli monocontaminated, and conventional baby pigs. Can J Comp Med. 1969 Apr;33(2):108–112. [PMC free article] [PubMed] [Google Scholar]
- Ferguson D. L., Mebus C. A., Twiehaus M. J. Recovery of porcine liver from damage caused by migrating x-irradiated and nonirradiated larvae of Ascaris suum. Am J Vet Res. 1968 Feb;29(2):233–243. [PubMed] [Google Scholar]
- GORDON H. A. Morphological and physiological characterization of germfree life. Ann N Y Acad Sci. 1959 May 8;78:208–220. doi: 10.1111/j.1749-6632.1959.tb53104.x. [DOI] [PubMed] [Google Scholar]
- Henderson J. D., Jr, Titus J. L. Growth rates and morphology of germfree and conventional mice. Mayo Clin Proc. 1968 Jul;43(7):517–529. [PubMed] [Google Scholar]
- Kruml J., Ludvík J., Trebichavský I., Mandel L., Kovárů F. Morphology of germ-free piglets. Folia Microbiol (Praha) 1969;14(5):441–446. doi: 10.1007/BF02872789. [DOI] [PubMed] [Google Scholar]
- MIYAKAWA M. The lymphatic system of germfree guinea pigs. Ann N Y Acad Sci. 1959 May 8;78:221–236. doi: 10.1111/j.1749-6632.1959.tb53105.x. [DOI] [PubMed] [Google Scholar]
- THORBECKE G. J. Some histological and functional aspects of lymphoid tissue in germfree animals. I. Morphological studies. Ann N Y Acad Sci. 1959 May 8;78:237–246. doi: 10.1111/j.1749-6632.1959.tb53106.x. [DOI] [PubMed] [Google Scholar]
- Waxler G. L., Schmidt D. A., Whitehair C. K. Technique for rearing gnotobiotic pigs. Am J Vet Res. 1966 Jan;27(116):300–307. [PubMed] [Google Scholar]