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. 1972 Jul;36(3):294–302.

Physical, Chemical and Biological Characteristics of Two Bovine Enterovirus Plaque Variants

H Rabin 1, J A Parsons 1, Judith Addison 1, C J York 1
PMCID: PMC1319684  PMID: 4340347

Abstract

Large plaque (4LP) and small plaque (4SP) variants were derived from a parent bovine virus strain by serial plaque passage. Both 4LP and 4SP were resistant to chloroform and stabilized at 50°C for one hour by 1.0 M magnesium chloride. Both 4LP and 4SP had buoyant densities in cesium chloride of 1.36 gm/ml. Antigenically, 4LP and 4SP were reciprocally cross neutralizable.

The nucleic acid of 4LP was shown to be ribonucleic acid (RNA) by resistance of its infectivity to deoxynuclease (DNase) but not ribonuclease (RNase) and by increased incorporation of [3H]-uridine into cytoplasmic RNA in cells of virus infected cultures. In growth characteristics, both 4LP and 4SP had maximum adsorption times of 75 to 90 minutes but 4LP had more rapid replication and release rates and yielded nearly twice as many infectious units per cell as 4SP. The differences in growth properties correlated directly with the differential in plaque diameter which was 40-50%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DARNELL J. E., Jr, SAWYER T. K. The basis for variation in susceptibility to poliovirus in HeLa cells. Virology. 1960 Aug;11:665–675. doi: 10.1016/0042-6822(60)90113-6. [DOI] [PubMed] [Google Scholar]
  2. FELDMAN H. A., WANG S. S. Sensitivity of various viruses to chloroform. Proc Soc Exp Biol Med. 1961 Apr;106:736–738. doi: 10.3181/00379727-106-26459. [DOI] [PubMed] [Google Scholar]
  3. HUCK R. A., CARTWRIGHT S. F. ISOLATION AND CLASSIFICATION OF VIRUSES FROM CATTLE DURING OUTBREAKS OF MUCOSAL OR RESPIRATORY DISEASE AND FROM HERDS WITH REPRODUCTIVE DISORDERS. J Comp Pathol. 1964 Jul;74:346–365. doi: 10.1016/s0368-1742(64)80041-2. [DOI] [PubMed] [Google Scholar]
  4. KUNIN C. M., MINUSE E. The isolation in tissue culture, chick embryo and suckling mice of filtrable agents from healthy dairy cattle. J Immunol. 1958 Jan;80(1):1–11. [PubMed] [Google Scholar]
  5. Kilham L. Viruses of laboratory and wild rats. Natl Cancer Inst Monogr. 1966 Feb;20:117–146. [PubMed] [Google Scholar]
  6. MADIN S. H., DARBY N. B., Jr Established kidney cell lines of normal adult bovine and ovine origin. Proc Soc Exp Biol Med. 1958 Jul;98(3):574–576. doi: 10.3181/00379727-98-24111. [DOI] [PubMed] [Google Scholar]
  7. MOLL T., ULRICH M. I. Biologic characteristics of certain bovine enteric viruses. Am J Vet Res. 1963 May;24:545–550. [PubMed] [Google Scholar]
  8. MOSCOVICI C., LAPLACA M., MAISEL J., KEMPE H. Studies of bovine enteroviruses. Am J Vet Res. 1961 Sep;22:852–863. [PubMed] [Google Scholar]
  9. Martin S. J., Johnston M. D., Clements J. B. Purification and characterization of bovine enteroviruses. J Gen Virol. 1970;7(2):103–113. doi: 10.1099/0022-1317-7-2-103. [DOI] [PubMed] [Google Scholar]
  10. Mayor H. D. Studies on reovirus. 3. A labile, single-stranded ribonucleic acid associated with the late stages of infection. J Natl Cancer Inst. 1965 Dec;35(6):919–925. [PubMed] [Google Scholar]
  11. NIEDERMAN R. A., LUGINBUHL R. E., HELMBOLDT C. F. THE SEPARATION AND CHARACTERIZATION OF TWO ENTERIC CYTOPATHIC BOVINE VIRUSES ISOLATED FROM A SAMPLE OF BOVINE FECES. Cornell Vet. 1963 Oct;53:550–560. [PubMed] [Google Scholar]
  12. Polson A., Kipps A. Physico-chemical investigation of an enteric cytopathogenic bovine orphan virus, ECBO SA.I. Arch Gesamte Virusforsch. 1965;17(3):488–494. doi: 10.1007/BF01241205. [DOI] [PubMed] [Google Scholar]
  13. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  14. TEMIN H. M., RUBIN H. Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture. Virology. 1958 Dec;6(3):669–688. doi: 10.1016/0042-6822(58)90114-4. [DOI] [PubMed] [Google Scholar]
  15. Van der Maaten M. J., Packer R. A. Isolation and characterization of bovine enteric viruses. Am J Vet Res. 1967 May;28(124):677–684. [PubMed] [Google Scholar]
  16. WALLIS C., MELNICK J. L. Stabilization of poliovirus by cations. Tex Rep Biol Med. 1961;19:683–700. [PubMed] [Google Scholar]

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