Skip to main content
PMC home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1973 Jul;37(3):217–223.

Antigen and Antibody in Aleutian Disease in Mink II. The Reaction of Antibody with the Aleutian Disease Agent Using Immunodiffusion and Immunoelectroosmophoresis

H J Cho 1, D G Ingram 1
PMCID: PMC1319762  PMID: 4270428

Abstract

Aleutian disease viral (ADV) antigen was prepared by fluorocarbon extraction of spleen, liver, and lymph nodes from mink experimentally infected ten days previously. Using a potent ADV antigen, antibody was detected by immunodiffusion (ID) and immunoelectroosmophoresis (IEOP). Utilizing these precipitin tests, antibody was detected in all the mink sera tested as early as seven days after experimental infection. Titer of antibody increased throughout the infection period. Titers of more than 100 were reached by 15 days post infection, titers of 1,000 at one month, and titers of more than 5,000 to 10,000 were achieved at two months post infection and thereafter. The immunodiffusion test gave similar or slightly lower titers than those detected by the IEOP.

The IEOP test promises to be a most useful technique for the diagnosis of aleutian disease because it is simple, rapid and specific and is capable of detecting infection early in the course of the disease. It is suggested that this test should be utilized especially for the screening of animals purchased or imported as breeding stock onto ranches.

Full text

PDF
217

Images in this article

220Fig. 1.
on p.220

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cho H. J., Ingram D. G. Antigen and antibody in Aleutian disease in mink. I. Precipitation reaction by agar-gel electrophoresis. J Immunol. 1972 Feb;108(2):555–557. [PubMed] [Google Scholar]
  2. GREENSPON S. A., KRAKOWER C. A. Direct evidence for the antigenicity of the glomeruli in the production of nephrotoxic serums. AMA Arch Pathol. 1950 Mar;49(3):291–297. [PubMed] [Google Scholar]
  3. Henson J. B., McGuire T. C. Immunopathology of equine infectious anemia. Am J Clin Pathol. 1971 Sep;56(3):306–313. doi: 10.1093/ajcp/56.3.306. [DOI] [PubMed] [Google Scholar]
  4. KOCHWA S., ROSENFIELD R. E. IMMUNOCHEMICAL STUDIES OF THE RH SYSTEM. I. ISOLATION AND CHARACTERIZATION OF ANTIBODIES. J Immunol. 1964 May;92:682–692. [PubMed] [Google Scholar]
  5. Kajima M., Pollard M. Ultrastructural pathology of glomerular lesions in gnotobiotic mice with congenital lymphocytic choriomeningitis (LCM) virus infection. Am J Pathol. 1970 Nov;61(2):117–140. [PMC free article] [PubMed] [Google Scholar]
  6. McGuire T. C., Crawford T. B., Henson J. B., Gorham J. R. Aleutian disease of mink: detection of large quantities of complement-fixing antibody to viral antigen. J Immunol. 1971 Nov;107(5):1481–1482. [PubMed] [Google Scholar]
  7. Mellors R. C., Huang C. Y. Immunopathology of NZB/BL mice. VI. Virus separable from spleen and pathogenic for Swiss mice. J Exp Med. 1967 Jul 1;126(1):53–62. doi: 10.1084/jem.126.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oldstone M. B., Dixon F. J. Pathogenesis of chronic disease associated with persistent lymphocytic choriomeningitis viral infection. I. Relationship of antibody production to disease in neonatally infected mice. J Exp Med. 1969 Mar 1;129(3):483–505. doi: 10.1084/jem.129.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PORTER D. D., DIXON F. J., LARSEN A. E. METABOLISM AND FUNCTION OF GAMMA GLOBULIN IN ALEUTIAN DISEASE OF MINK. J Exp Med. 1965 Jun 1;121:889–900. doi: 10.1084/jem.121.6.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PORTER D. D., DIXON F. J., LARSEN A. E. THE DEVELOPMENT OF A MYELOMA-LIKE CONDITION IN MINK WITH ALEUTIAN DISEASE. Blood. 1965 May;25:736–742. [PubMed] [Google Scholar]
  11. Pan I. C., De Boer C. J., Heuschele W. P. Hypergammaglobulinemia in swine infected with African swine fever virus. Proc Soc Exp Biol Med. 1970 Jun;134(2):367–371. doi: 10.3181/00379727-134-34794. [DOI] [PubMed] [Google Scholar]
  12. Porter D. D., Larsen A. E., Porter H. G. The pathogenesis of Aleutian disease of mink. I. In vivo viral replication and the host antibody response to viral antigen. J Exp Med. 1969 Sep 1;130(3):575–593. doi: 10.1084/jem.130.3.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schmidt N. J., Gee P. S., Lennette E. H. Relative sensitivity of gel diffusion, complement fixation, and immunoelectroosmophoresis tests for detection of hepatitis-associated antigen and antibody. Appl Microbiol. 1971 Aug;22(2):165–170. doi: 10.1128/am.22.2.165-170.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tabel H., Ingram D. G. The immunoglobulins in Aleutian disease (viral plasmacytosis) of mink. Different types of hypergammaglobulinemias. Can J Comp Med. 1970 Oct;34(4):329–332. [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES