Abstract
Aerosols of bovine parainfluenza type 3 virus were generated with a Devilbiss 40 nebulizer from Eagle's minimum essential medium and nasal secretion from a non-infected calf and stored in a rotating drum at temperatures of 6 degrees C or 32 degrees C and relative humidities of 30% or 90%. The aerosols were sampled at seven minutes, one, two and three hours after the start of generation with an all glass impinger (AGI-30) and titrated for infectivity in cell cultures. Physical decay was determined by a rhodamine tracer technique. Media, temperature or relative humidity had little effect on the survival of parainfluenza type 3 virus during spraying (zero to seven minutes). During aging of aerosols at 32 degrees C and 30% relative humidity, parainfluenza type 3 virus was less stable in Eagle's minimum essential medium than in nasal secretion from a noninfected calf, but at 6 degrees C and 30% relative humidity, the virus was more stable in Eagle's minimum essential medium. At 32 degrees C, the virus was less stable during aging at 90% relative humidity than at 30% relative humidity. The virus was consistently more stable during aging of aerosols at 6 degrees C than at 32 degrees C.
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- Akers T. G., Prato C. M., Dubovi E. J. Airborne stability of simian virus 40. Appl Microbiol. 1973 Aug;26(2):146–148. doi: 10.1128/am.26.2.146-148.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE JONG J. G., WINKLER K. C. SURVIVAL OF MEASLES VIRUS IN AIR. Nature. 1964 Mar 7;201:1054–1055. doi: 10.1038/2011054a0. [DOI] [PubMed] [Google Scholar]
- Ehrlich R., Miller S. Effect of relative humidity and temperature on airborne Venezuelan equine encephalitis virus. Appl Microbiol. 1971 Aug;22(2):194–199. doi: 10.1128/am.22.2.194-199.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elazhary M. A., Derbyshire J. B. Aerosol stability of bovine adenovirus type 3. Can J Comp Med. 1979 Jul;43(3):305–312. [PMC free article] [PubMed] [Google Scholar]
- Elazhary M. A., Derbyshire J. B. Effect of temperature, relative humidity and medium on the aerosol stability of infectious bovine rhinotracheitis virus. Can J Comp Med. 1979 Apr;43(2):158–167. [PMC free article] [PubMed] [Google Scholar]
- HEMMES J. H., WINKLER K. C., KOOL S. M. Virus survival as a seasonal factor in influenza and polimyelitis. Nature. 1960 Oct 29;188:430–431. doi: 10.1038/188430a0. [DOI] [PubMed] [Google Scholar]
- Haliburton G., Becker M. E. The use of HEPES buffer in micro-tissue culture plates for routine enterovirus diagnosis. Health Lab Sci. 1971 Jul;8(3):155–159. [PubMed] [Google Scholar]
- Hamdy A. H. Association of myxovirus parainfluenza-3 with pneumoenteritis of calves: virus isolation. Am J Vet Res. 1966 Jul;27(119):981–986. [PubMed] [Google Scholar]
- Miller W. S., Artenstein M. S. Aerosol stability of three acute respiratory disease viruses. Proc Soc Exp Biol Med. 1967 May;125(1):222–227. doi: 10.3181/00379727-125-32054. [DOI] [PubMed] [Google Scholar]
- REISINGER R. C., HEDDLESTON K. L., MANTHEI C. A. A myxovirus (SF-4) associated with shipping fever of cattle. J Am Vet Med Assoc. 1959 Aug 1;135(3):147–152. [PubMed] [Google Scholar]
- Rouse B. T., Angulo A. B. A method for the collection of nasal secretions from the horse and cow. Res Vet Sci. 1970 Jan;11(1):98–99. [PubMed] [Google Scholar]
- TYLER M. E., SHIPE E. L. Bacterial aerosol samplers. I. Development and evaluation of the all-glass impinger. Appl Microbiol. 1959 Nov;7:337–349. doi: 10.1128/am.7.6.337-349.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Jong J. C., Harmsen M., Trouwborst T. Factors in the inactivation of Encephalomyocarditis virus in aerosols. Infect Immun. 1975 Jul;12(1):29–35. doi: 10.1128/iai.12.1.29-35.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]