Abstract
A countercurrent immunoelectrophoresis test was developed for the detection of precipitating antibodies to pseudorabies virus in pig serum. The precipitation reaction occurred only between the pseudorables antigen and the homologous porcine antiserum. The sensitivity of the method was compared to that of the serum neutralization test. On the basis of its sensitivity, its specificity and the rapidity with which the results were obtained, the countercurrent immunoelectrophoresis may become a potentially valuable screening method to test large numbers of porcine serum.
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- Anderson E. C., Capstick P. B., Mowat G. N., Leech F. B. In vitro method for safety testing of foot-and-mouth disease vaccines. J Hyg (Lond) 1970 Jun;68(2):159–172. doi: 10.1017/s0022172400028643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cho H. J., Ingram D. G. Antigen and antibody in Aleutian disease in mink. I. Precipitation reaction by agar-gel electrophoresis. J Immunol. 1972 Feb;108(2):555–557. [PubMed] [Google Scholar]
- Golding S. M., Hedger R. S., Talbot P. Radial immuno-diffusion and serum-neutralisation techniques for the assay of antibodies to swine vesicular disease. Res Vet Sci. 1976 Mar;20(2):142–147. [PubMed] [Google Scholar]
- Greig A. S., Bannister G. L., Mitchell D., Corner A. H. Studies on Pathogenic Porcine Enteroviruses: II. Isolation of Virus in Tissue Culture from Brain and Feces of Clinical Cases. Can J Comp Med Vet Sci. 1961 Jun;25(6):142–150. [PMC free article] [PubMed] [Google Scholar]
- Gutekunst D. E., Pirtle E. C., Mengeling W. L. Development and evaluation of a microimmunodiffusion test for detection of antibodies to pseudorabies virus in swine serum. Am J Vet Res. 1978 Feb;39(2):207–210. [PubMed] [Google Scholar]
- MCFERRAN J. B., DOW C. THE DISTRIBUTION OF THE VIRUS OF AUJESZKY'S DISEASE (PSEUDORABIES VIRUS) IN EXPERIMENTALLY INFECTED SWINE. Am J Vet Res. 1965 May;26:631–635. [PubMed] [Google Scholar]
- Mitchell A. G. Production de Porcs Exempts d'Organismes Pathogenes (EOPS) Primaires et Etablissement d'un Troupeau de Porcs EOPS Secondaires. Can J Comp Med Vet Sci. 1962 Dec;26(12):279–284. [PMC free article] [PubMed] [Google Scholar]
- Mowat G. N., Prince M. J., Spier R. E., Staple R. F. Preliminary studies on the development of a swine vesicular disease vaccine. Arch Gesamte Virusforsch. 1974;44(4):350–360. doi: 10.1007/BF01251016. [DOI] [PubMed] [Google Scholar]
- Watson D. H., Wildy P., Harvey B. A., Shedden W. I. Serological relationships among viruses of the herpes group. J Gen Virol. 1967 Jan;1(1):139–141. doi: 10.1099/0022-1317-1-1-139. [DOI] [PubMed] [Google Scholar]