Abstract
A direct fluorescent antibody test was applied to the diagnosis of avian encephalomyeltis in experimental chicks and in chicks from three natural outbreaks of the disease. Intracerebrally inoculated chicks exhibited maximum fluorescence in brain tissue during the early clinical stages of the disease and similar observations were made on natural cases. Best fluorescence was obtained with frozen sections of cerebellum and pancreas from chicks approximately three weeks of age, but after four weeks of age it was not possible to diagnose the naturally-occurring (field) disease by this method. In these cases a diagnosis of avian encephalomyelitis was confirmed by the application of the fluorescent technique to the brains of either (a) chicks which had hatched from eggs inoculated with brain homogenates from the field cases or (b) hatched chicks which had been inoculated with the homogenates by the intracerebral route.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Braune M. O., Gentry R. F. Avian encephalomyelitis virus. I. Pathogenesis in chicken embryos. Avian Dis. 1971 Oct-Dec;15(4):638–647. [PubMed] [Google Scholar]
- COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FELDMAN H. A., WANG S. S. Sensitivity of various viruses to chloroform. Proc Soc Exp Biol Med. 1961 Apr;106:736–738. doi: 10.3181/00379727-106-26459. [DOI] [PubMed] [Google Scholar]
- Lawson K. F., Gregg W. A. A report on the wing web method of vaccination against avian encephalomyelitis using the CAE strain. Avian Dis. 1969 Nov;13(4):699–708. [PubMed] [Google Scholar]
- MAESTRONE G., COFFIN D. L. STUDY OF NEWCASTLE DISEASE BY MEANS OF FLUORESCENT ANTIBODY TECHNIQUE. Am J Vet Res. 1964 Jan;25:217–223. [PubMed] [Google Scholar]
- Robertson A., Greig A. S., Appel M., Girard A., Bannister G. L., Boulanger P. Hog cholera IV. Detection of the virus in tissue culture preparations by the fluorescent antibody technique. Can J Comp Med Vet Sci. 1965 Sep;29(9):234–241. [PMC free article] [PubMed] [Google Scholar]
- Van Der Heide L., Chute H. L., O'Meara D. C. A comparative investigation of the course of an experimental infection with infectious laryngotracheitis in chickens. Avian Dis. 1967 May;11(2):149–153. [PubMed] [Google Scholar]