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. 2026 Apr 30;29(6):115958. doi: 10.1016/j.isci.2026.115958

Figure 2.

Figure 2

Expression analysis of BVRA missense mutants G17A and E97A in C57BL/6J mice

(A) RT-qPCR analysis of Blvra mRNA expression in spleen, kidney, and liver from BlvraWT, Blvra−/− and BlvraG17A mice. Data are expressed as Blvra/Rplp0 (2−ΔCt) and presented as individual data points with bar graphs showing mean ± SD. n = 3 mice per genotype.

(B) Western blot analysis of BVRA protein expression in spleen, kidney, and liver. Representative blots show BVRA (33 kDa) and β-actin (42 kDa) loading control. Quantification of BVRA protein levels normalized to β-actin is shown below each blot as individual data points with bar graphs showing mean ± SD. n = 3–5 per genotype.

(C) RT-qPCR analysis of Blvra mRNA expression in spleen, kidney and liver tissues from BlvraWT, Blvra−/−, and BlvraE97A mice. Data are expressed as Blvra/Rplp0 (2−ΔCt) and presented as individual data points with bar graphs showing mean ± SD. n = 3 per genotype.

(D) Western blot analysis of BVRA protein expression in spleen, kidney, and liver tissue. Representative blots and quantification of BVRA protein levels normalized to β-actin below each blot, shown as individual data points with bar graphs showing mean ± SD. n = 3–6 mice per genotype. Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; NS, not significant.