Abstract
Whole cells of Brucella abortus strain 45/20 were combined with trehalose dimycolate or muramyl dipeptide. These preparations were tested in guinea pigs for immunogenic properties. Both trehalose dimycolate and muramyl dipeptide were found to be effective adjuvants to whole cells when combined in oil emulsions. Although saline suspensions of whole cells or whole cells-muramyl dipeptide did not significantly reduce splenic infections, oil emulsion or whole cells-trehalose dimycolate in oil droplet emulsion were both effective immunogens (P < 0.05). Oil emulsions of whole cells-muramyl dipeptide reduced mean splenic Brucella by 95.1% and those of whole cells-trehalose dimycolate reduced mean splenic Brucella by 99.3% as compared to the control animals.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Emori K., Tanaka A. Granuloma formation by synthetic bacterial cell wall fragment: muramyl dipeptide. Infect Immun. 1978 Feb;19(2):613–620. doi: 10.1128/iai.19.2.613-620.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACKANESS G. B. THE IMMUNOLOGICAL BASIS OF ACQUIRED CELLULAR RESISTANCE. J Exp Med. 1964 Jul 1;120:105–120. doi: 10.1084/jem.120.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McLaughlin C. A., Parker R., Hadlow W. J., Toubiana R., Ribi E. Moieties of mycobacterial mycolates required for inducing granulomatous reactions. Cell Immunol. 1978 Jun;38(1):14–24. doi: 10.1016/0008-8749(78)90027-8. [DOI] [PubMed] [Google Scholar]
- Woodard L. F., Toone N. M., McLaughlin C. A. Immunogenic properties of soluble antigens or whole cells of Brucella abortus strain 45/20 associated with immunoadjuvants. I. Soluble antigens. Can J Comp Med. 1980 Oct;44(4):453–455. [PMC free article] [PubMed] [Google Scholar]