Abstract
The application of the indirect enzyme-linked immunosorbent assay (ELISA) for detection of infectious bursal disease virus antibodies in chicken serum was investigated. The test procedure involved the coating of concentrated infectious bursal disease virus antigen onto polystyrene tubes, followed by the addition of chicken anti-infectious bursal disease virus serum and horseradish peroxidase labeled rabbit anti-chicken globulin. As an indicator substrate, 5-aminosalicylic acid, with the oxidant H2O2 was added. The reaction was stopped by 3M NaOH and the colour intensity of the reaction mixtures read in a spectrophotometer at 449 nm. The ELISA test was found to be a precise, sensitive and reproducible means of measuring infectious bursal disease virus antibodies in chicken and turkey sera.
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- Bullock S. L., Walls K. W. Evaluation of some of the parameters of the enzyme-linked immunospecific assay. J Infect Dis. 1977 Oct;136 (Suppl):S279–S285. doi: 10.1093/infdis/136.supplement_2.s279. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Ghose L. H., Schnagl R. D., Holmes I. H. Comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey. J Clin Microbiol. 1978 Sep;8(3):268–276. doi: 10.1128/jcm.8.3.268-276.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilman S. C., Docherty J. J. Detection of antibodies specific for herpes simplex virus in human sera by the enzyme-linked immunosorbent assay. J Infect Dis. 1977 Oct;136 (Suppl):S286–S293. doi: 10.1093/infdis/136.supplement_2.s286. [DOI] [PubMed] [Google Scholar]
- Miller M. H., Karnovsky M. J., Diamandopoulos G. T. An improved immunoperoxidase technique for identifying SV40 V and T antigens by light microscopy. Proc Soc Exp Biol Med. 1974 Jun;146(2):432–437. doi: 10.3181/00379727-146-38120. [DOI] [PubMed] [Google Scholar]
- Moutou F., Toma B., Fortier B. Application of an enzyme linked immunosorbent assay for diagnosis of Aujeszky's disease in swine. Vet Rec. 1978 Sep 16;103(12):264–264. doi: 10.1136/vr.103.12.264. [DOI] [PubMed] [Google Scholar]
- Saunders G. C., Clinard E. H., Bartlett M. L., Sanders W. M. Application of the indirect enzyme-labeled antibody microtest to the detection and surveillance of animal diseases. J Infect Dis. 1977 Oct;136 (Suppl):S258–S266. doi: 10.1093/infdis/136.supplement_2.s258. [DOI] [PubMed] [Google Scholar]