Abstract
Two methods are described for the partial purification of a high molecular weight, heat-resistant component (CO1) of sonicates of smooth and rough Brucella abortus which is precipitated by sera of some infected cattle. Method 1, a combination of gel filtration chromatography and polyacrylamide gel electrophoresis, was used to prepare CO1 from sonicates of a smooth field strain of B. abortus. Method 2, a combination of gel filtration chromatography and heat treatment, was used to obtain CO1, from sonicates of rough B. abortus strain 45/20. Rabbit antisera produced against CO1 prepared by either method contained only CO1 precipitins but were negative in standard agglutination and complement fixation tests conducted with whole cell antigens. Evidence is presented that CO1 is identical to Brucella antigen A2, and it is proposed that in future the designation A2 be employed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Corbel M. J. Evaluation of an immunodiffusion test for the detection of antibodies to Brucella abortus in bovine serum. J Med Microbiol. 1973 Feb;6(1):67–76. doi: 10.1099/00222615-6-1-67. [DOI] [PubMed] [Google Scholar]
- Diaz R., Garatea P., Jones L. M., Moriyon I. Radial immunodiffusion test with a Brucella polysaccharide antigen for differentiating infected from vaccinated cattle. J Clin Microbiol. 1979 Jul;10(1):37–41. doi: 10.1128/jcm.10.1.37-41.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iannelli D., Diaz R., Bettini T. M. Identification of Brucella abortus antibodies in cattle serum by single radial diffusion. J Clin Microbiol. 1976 Feb;3(2):203–205. doi: 10.1128/jcm.3.2.203-205.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Malkin K. L., Tailyour J. M., Bhatia T. R., Archibald R. M., Dorward W. J. A serological survey for brucellosis in Canadian swine. Can J Comp Med. 1968 Oct;32(4):598–599. [PMC free article] [PubMed] [Google Scholar]
- Nielsen K., Samagh B. S., Speckmann G., Stemshorn B. The bovine immune response to Brucella abortus. II. Elimination of some sporadic serological reactions by chelation of divalent cations. Can J Comp Med. 1979 Oct;43(4):420–425. [PMC free article] [PubMed] [Google Scholar]
- Schurig G. G., Jones L. M., Speth S. L., Berman D. T. Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection. Infect Immun. 1978 Sep;21(3):994–1002. doi: 10.1128/iai.21.3.994-1002.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stemshorn B., Nielsen K. The bovine immune response to Brucella abortus I. A water soluble antigen precipitated by sera of some naturally infected cattle. Can J Comp Med. 1977 Apr;41(2):152–159. [PMC free article] [PubMed] [Google Scholar]