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. 1981 Apr;45(2):135–139.

Neonatal bovine enteritis: detection of rotavirus by counterimmunoelectrophoresis and enzyme-linked immunosorbent assay.

L J Ekern, I A Schipper, K J McMahon
PMCID: PMC1320140  PMID: 6266620

Abstract

Counterimmunoelectrophoresis and enzyme-linked immunosorbent assay specific for rotavirus were performed on 253 calf gut and fecal specimens from eastern North Dakota. Rotavirus antigen was detected in 29 specimens by counterimmunoelectrophoresis and in 52 specimens by enzyme-linked immunosorbent assay. All of the counterimmunoelectrophoresis-positive specimens were positive by enzyme-linked immunosorbent assay. The counterimmunoelectrophoresis and the enzyme-linked immunosorbent assay, using a rabbit antirotavirus antibodies produced a golf ball-induced granuloma, were rapid and specific tests for the detection of rotavirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. Rotavirus isolation and cultivation in the presence of trypsin. J Clin Microbiol. 1977 Dec;6(6):610–617. doi: 10.1128/jcm.6.6.610-617.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bryden A. S., Davies H. A., Thouless M. E., Flewitt T. H. Diagnosis of rotavirus infection by cell culture. J Med Microbiol. 1977 Feb;10(1):121–125. doi: 10.1099/00222615-10-1-121. [DOI] [PubMed] [Google Scholar]
  3. Bullock S. L., Walls K. W. Evaluation of some of the parameters of the enzyme-linked immunospecific assay. J Infect Dis. 1977 Oct;136 (Suppl):S279–S285. doi: 10.1093/infdis/136.supplement_2.s279. [DOI] [PubMed] [Google Scholar]
  4. Ellens D. J., de Leeuw P. W. Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves. J Clin Microbiol. 1977 Nov;6(5):530–532. doi: 10.1128/jcm.6.5.530-532.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellens D. J., de Leeuw P. W., Straver P. J., van Balken J. A. Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces. Med Microbiol Immunol. 1978 Nov 17;166(1-4):157–163. doi: 10.1007/BF02121145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. England J. J., Frye C. S., Enright E. A. Negative contrast electron microscopic diagnosis of viruses of neonatal calf diarrhea. Cornell Vet. 1976 Apr;66(2):172–182. [PubMed] [Google Scholar]
  7. Grauballe P. C., Genner J., Meyling A., Hornsleth A. Rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis. J Gen Virol. 1977 May;35(2):203–218. doi: 10.1099/0022-1317-35-2-203. [DOI] [PubMed] [Google Scholar]
  8. Hillam R. P., Tengerdy R. P., Brown G. L. Local antibody production against the murine toxin of Yersinia pestis in a golf ball-induced granuloma. Infect Immun. 1974 Sep;10(3):458–463. doi: 10.1128/iai.10.3.458-463.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kalica A. R., James J. D., Jr, Kapikian A. Z. Hemagglutination by simian rotavirus. J Clin Microbiol. 1978 Mar;7(3):314–315. doi: 10.1128/jcm.7.3.314-315.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kalica A. R., Purcell R. H., Sereno M. M., Wyatt R. G., Kim H. W., Chanock R. M., Kapikian A. Z. A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools. J Immunol. 1977 Apr;118(4):1275–1279. [PubMed] [Google Scholar]
  11. Middleton P. J., Petric M., Hewitt C. M., Szymanski M. T., Tam J. S. Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody. J Clin Pathol. 1976 Mar;29(3):191–197. doi: 10.1136/jcp.29.3.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mills K. W., Gerlach E. H., Bell J. W., Farkas M. E., Taylor R. J. Serotyping herpes simplex virus isolated by enzyme-linked immunosorbent assays. J Clin Microbiol. 1978 Jan;7(1):73–76. doi: 10.1128/jcm.7.1.73-76.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rhodes M. B., Stair E. L., McCullough R. A., McGill L. D., Mebus C. A. Comparison of results using electron microscope, immunodiffusion and fluorescent antibody analyses to detect rotavirus in diarrheic fecal samples of calves. Can J Comp Med. 1979 Jan;43(1):84–89. [PMC free article] [PubMed] [Google Scholar]
  14. Woode G. N., Bridger J. C. Viral enteritis of calves. Vet Rec. 1975 Jan 25;96(4):85–88. doi: 10.1136/vr.96.4.85. [DOI] [PubMed] [Google Scholar]
  15. Yolken R. H., Barbour B., Wyatt R. G., Kalica A. R., Kapikian A. Z., Chanock R. M. Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species. Science. 1978 Jul 21;201(4352):259–262. doi: 10.1126/science.208150. [DOI] [PubMed] [Google Scholar]

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