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. 1981 Apr;45(2):140–146.

The application of the soluble antigen fluorescent antibody test for the diagnosis of avian influenza.

M Al-Attar, K Nielsen, W R Mitchell
PMCID: PMC1320142  PMID: 7020892

Abstract

The application of the soluble antigen fluorescent test as a tool for serological investigation of influenza type A infection in wild birds was studied. The soluble antigen fluorescent antibody test is basically an indirect fluorescent antibody test except that an artificial matrix of cellulose acetate discs is used as a substrate for antigen and the test results are scanned and recorded by a fluorometer. THe influenza type A soluble antigen fluorescent antibody was obtained from concentrated and detergent disrupted virus particles, absorbed onto cellulose acetate discs. Anti-influenza sera were prepared in pheasants and ducks to A/turkey/Ontario/6118/67 and in pigeons to A/turkey/Ontario/6213/68. The antigen-antibody complex was detected by specific staining with monovalent or polyvalent fluorescein isothiocyanate conjugated rabbit anti-avian immunoglobulins. The soluble antigen fluorescent antibody test is a sensitive technique for the detection of specific influenza A antibodies in several avian species, and could be adapted for use in large scale surveys.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artenstein M. S., Dandridge O. W. A new serologic test for adenovirus infection. J Immunol. 1968 Apr;100(4):831–834. [PubMed] [Google Scholar]
  2. Beard C. W. Demonstration of type-specific influenza antibody in mammalian and avian sera by immunodiffusion. Bull World Health Organ. 1970;42(5):779–785. [PMC free article] [PubMed] [Google Scholar]
  3. Becker W. B. The isolation and classification of Tern virus: influenza A-Tern South Africa--1961. J Hyg (Lond) 1966 Sep;64(3):309–320. doi: 10.1017/s0022172400040596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dowdle W. R., Galphin J. C., Coleman M. T., Schild G. C. A simple double immunodiffusion test for typing influenza viruses. Bull World Health Organ. 1974;51(3):213–215. [PMC free article] [PubMed] [Google Scholar]
  5. Downie J. C., Laver W. G. Isolation of a type A influenza virus from an Australian pelagic bird. Virology. 1973 Feb;51(2):259–269. doi: 10.1016/0042-6822(73)90426-1. [DOI] [PubMed] [Google Scholar]
  6. Easterday B. C., Trainer D. O., Tůmová B., Pereira H. G. Evidence of infection with influenza viruses in migratory waterfowl. Nature. 1968 Aug 3;219(5153):523–524. doi: 10.1038/219523a0. [DOI] [PubMed] [Google Scholar]
  7. Garnham J. A., Wilkie B. N., Nielsen K., Thorsen J. Application of the soluble antigen fluorescent antibody (SAFA) test to the serodiagnosis of rabies. J Immunol Methods. 1977;14(2):147–162. doi: 10.1016/0022-1759(77)90005-9. [DOI] [PubMed] [Google Scholar]
  8. Hebert G. A. Ammonium sulfate fractionation of sera: mouse, hamster, guinea pig, monkey, chimpanzee, swine, chicken, and cattle. Appl Microbiol. 1974 Feb;27(2):389–393. doi: 10.1128/am.27.2.389-393.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson D. C., Maxfield B. G. An occurrence of avian influenza virus infection in laying chickens. Avian Dis. 1976 Apr-Jun;20(2):422–424. [PubMed] [Google Scholar]
  10. Lang G., Ferguson A. E., Connell M. C., Wills C. G. Isolation of an unidentified hemagglutinating virus from the respiratory tract of turkeys. Avian Dis. 1965 Nov;9(4):495–504. [PubMed] [Google Scholar]
  11. Lang G., Rouse B. T., Narayan O., Ferguson A. E., Connell M. C. A new influenza virus infection in turkeys. I. Isolation and characterization of virus 6213. Can Vet J. 1968 Jan;9(1):22–29. [PMC free article] [PubMed] [Google Scholar]
  12. Pereira H. G., Rinaldi A., Nardelli L. Antigenic variation among avian influenza A viruses. Bull World Health Organ. 1967;37(4):553–558. [PMC free article] [PubMed] [Google Scholar]
  13. Slemons R. D., Cooper R. S., Orsborn J. S. Isolation of type-A influenza viruses from imported exotic birds. Avian Dis. 1973 Oct-Dec;17(4):746–751. [PubMed] [Google Scholar]
  14. Slemons R. D., Johnson D. C., Osborn J. S., Hayes F. Type-A influenza viruses isolated from wild free-flying ducks in California. Avian Dis. 1974 Jan-Mar;18(1):119–124. [PubMed] [Google Scholar]
  15. TOUSSAINT A. J., ANDERSON R. I. SOLUBLE ANTIGEN FLUORESCENT-ANTIBODY TECHNIQUE. Appl Microbiol. 1965 Jul;13:552–558. doi: 10.1128/am.13.4.552-558.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Webster R. G., Morita M., Pridgen C., Tumova B. Ortho- and paramyxoviruses from migrating feral ducks: characterization of a new group of influenza A viruses. J Gen Virol. 1976 Aug;32(2):217–225. doi: 10.1099/0022-1317-32-2-217. [DOI] [PubMed] [Google Scholar]
  17. Wells A. F., Miller C. E., Nadel M. K. Rapid fluorescein and protein assay method for fluorescent-antibody conjugates. Appl Microbiol. 1966 Mar;14(2):271–275. doi: 10.1128/am.14.2.271-275.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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