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. 1981 Apr;45(2):147–153.

The bovine immune response to Brucella abortus IV. Studies with a double immunodiffusion test for antibody against A2.

B Stemshorn, K Nielsen
PMCID: PMC1320143  PMID: 6790144

Abstract

A double immunodiffusion test for precipitins against Brucella antigen A2 was developed and applied to a variety of samples. The A2 precipitins were produced by a heifer infected with B. abortus strain 2308, cattle vaccinated with killed B. melitensis strain H38 or live B. abortus strain 19 and by a dog infected with B. canis. Precipitins were also detected in the second International Standard for anti-Brucella abortus serum, in several anti-B. canis sera and at low levels in one anti-B. ovis serum tested. Antisera produced in calves against Yersinia enterocolitica serotype 0:9 had no anti-A2 activity despite titers greater than or equal to 1/1024 and greater than or equal to 1/80 in standard Brucella agglutination and CF tests, respectively. The test for A2 precipitins lacked specificity as weak reactions were obtained with five of 295 sera from brucellosis-free herds. This test was relatively insensitive, detecting precipitins in only 16 of 24 sera from infected cattle and 27 of 54 sera positive by complement fixation and enzyme labelled antiglobulin tests performed with whole cell and smooth lipopolysaccharide antigens, respectively. The A2 precipitins were detected in nine sera from five cattle, in two infected herds, which were negative by agglutination and complement fixation tests.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRUCE W., JONES L. M. The use of gel diffusion precipitin plates in the study of Brucella. Bull World Health Organ. 1958;19(1):187–196. [PMC free article] [PubMed] [Google Scholar]
  2. Corbel M. J. Evaluation of an immunodiffusion test for the detection of antibodies to Brucella abortus in bovine serum. J Med Microbiol. 1973 Feb;6(1):67–76. doi: 10.1099/00222615-6-1-67. [DOI] [PubMed] [Google Scholar]
  3. Corbel M. J., Scott A. C., Ross H. M. Properties of a cell-wall-defective variant of Brucella abortus of bovine origin. J Hyg (Lond) 1980 Aug;85(1):103–113. doi: 10.1017/s002217240002711x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davidson I., Hebert C. N., Morgan W. J. The Second International Standard for anti-Brucella abortus serum. Bull World Health Organ. 1969;40(1):129–140. [PMC free article] [PubMed] [Google Scholar]
  5. Eardley D. D., Jayawardena A. N. Suppressor cells in mice infected with Trypanosoma brucei. J Immunol. 1977 Sep;119(3):1029–1033. [PubMed] [Google Scholar]
  6. GLENCHUR H., SEAL U. S., ZINNEMAN H. H., HALL W. H. Serum precipitins in human and experimental brucellosis. J Lab Clin Med. 1962 Feb;59:220–230. [PubMed] [Google Scholar]
  7. Hinsdill R. D., Berman D. T. Antigens of Brucella abortus. I. Chemical and immunoelectrophoretic characterization. J Bacteriol. 1967 Feb;93(2):544–549. doi: 10.1128/jb.93.2.544-549.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iannelli D., Diaz R., Bettini T. M. Identification of Brucella abortus antibodies in cattle serum by single radial diffusion. J Clin Microbiol. 1976 Feb;3(2):203–205. doi: 10.1128/jcm.3.2.203-205.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LeJeune J. M., Hart L. T., Larson A. D., Seger C. L. Microimmunodiffusion test for detection of antibodies to infectious bovine rhinotracheitis virus in bovine serum. Am J Vet Res. 1977 Apr;38(4):459–463. [PubMed] [Google Scholar]
  10. Malkin K. L., Tailyour J. M., Bhatia T. R., Archibald R. M., Dorward W. J. A serological survey for brucellosis in Canadian swine. Can J Comp Med. 1968 Oct;32(4):598–599. [PMC free article] [PubMed] [Google Scholar]
  11. Mansfield J. M., Wallace J. H. Suppression of cell-mediated immunity in experimental African trypanosomiasis. Infect Immun. 1974 Aug;10(2):335–339. doi: 10.1128/iai.10.2.335-339.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meloen R. H. Differentiation between specific and nonspecific reactions of bovine sera and foot and mouth disease virus (FMDV) in immunodiffusion tests. Arch Virol. 1978;58(1):35–43. doi: 10.1007/BF01315533. [DOI] [PubMed] [Google Scholar]
  13. Nagy L. K. Studies on the precipitin content of bovine sera prepared against various biotypes of Brucella abortus. Immunology. 1967 Apr;12(4):463–474. [PMC free article] [PubMed] [Google Scholar]
  14. Ross H. M. Isolation of a cell-wall-defective strain of Brucella abortus from bovine tissue. Vet Rec. 1980 Mar 15;106(11):247–247. doi: 10.1136/vr.106.11.247. [DOI] [PubMed] [Google Scholar]
  15. Schurig G. G., Jones L. M., Speth S. L., Berman D. T. Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection. Infect Immun. 1978 Sep;21(3):994–1002. doi: 10.1128/iai.21.3.994-1002.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stemshorn B. W., Nielsen K. H., Samagh B. S., Forbes L. B., Ingram D. G. Evaluation of an enzyme-labeled antiglobulin test for anti-Brucella immunoglobulin G among 3 cattle populations. Am J Vet Res. 1980 Nov;41(11):1779–1784. [PubMed] [Google Scholar]
  17. Stemshorn B., Nielsen K., Samagh B. The bovine immune response to Brucella abortus. III. Preparation of antisera against a Brucella component precipitated by sera of some infected cattle. Can J Comp Med. 1981 Jan;45(1):77–81. [PMC free article] [PubMed] [Google Scholar]
  18. Stemshorn B., Nielsen K. The bovine immune response to Brucella abortus I. A water soluble antigen precipitated by sera of some naturally infected cattle. Can J Comp Med. 1977 Apr;41(2):152–159. [PMC free article] [PubMed] [Google Scholar]

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