Skip to main content
NCBI home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1982 Jan;46(1):70–75.

The pathogenicity of Yersinia enterocolitica strains isolated from various sources in four test systems.

F Mosimabale, C L Gyles
PMCID: PMC1320199  PMID: 7042054

Abstract

Six tests were applied to 39 strains of Yersinia enterocolitica of various serotypes and from several sources in an attempt to relate the test to pathogenicity of the strains. The tests that were used were the pig gut loop test and the infant mouse test for heat stable enterotoxin, the Sereny and HeLa cell tests for invasiveness, inhibition of growth on magnesium oxalate agar, and the ability to cause diarrhea in infant mice. The pig gut loop test was found to be unsuitable for detection of heat stable enterotoxin but 20 strains produced heat stable enterotoxin that was detected in infant mice. None of the strains was positive in the Sereny test but 21 invaded HeLa cells. The growth of 20 strains was inhibited at 37 degrees C on magnesium oxalate agar and, in the orally-infected mice, 23 strains caused diarrhea or death. These findings indicate a discrepancy between the infant mouse test and the ligated intestine test in pigs for heat stable enterotoxin and a significant difference in Y. enterocolitica heat stable enterotoxin compared with Escherichia coli heat stable enterotoxin because the former failed to elicit a significant response in pig intestine.

Full text

PDF
70

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahvonen P. Human yersiniosis in Finland. II. Clinical features. Ann Clin Res. 1972 Feb;4(1):39–48. [PubMed] [Google Scholar]
  2. Boyce J. M., Evans E. J., Jr, Evans D. G., DuPont H. L. Production of heat-stable, methanol-soluble enterotoxin by Yersinia enterocolitica. Infect Immun. 1979 Aug;25(2):532–537. doi: 10.1128/iai.25.2.532-537.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Braunstein H., Tucker E. B., Gibson B. C. Mesenteric lymphadenitis due to Yersinia enterocolitica: report of a case. Am J Clin Pathol. 1971 Apr;55(4):506–510. doi: 10.1093/ajcp/55.4.506. [DOI] [PubMed] [Google Scholar]
  4. Cantey J. R., Blake R. K. Diarrhea due to Escherichia coli in the rabbit: a novel mechanism. J Infect Dis. 1977 Mar;135(3):454–462. doi: 10.1093/infdis/135.3.454. [DOI] [PubMed] [Google Scholar]
  5. Carter P. B., Collins F. M. Experimental Yersinia enterocolitica infection in mice: kinetics of growth. Infect Immun. 1974 May;9(5):851–857. doi: 10.1128/iai.9.5.851-857.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gemski P., Lazere J. R., Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980 Feb;27(2):682–685. doi: 10.1128/iai.27.2.682-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Giannella R. A. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976 Jul;14(1):95–99. doi: 10.1128/iai.14.1.95-99.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Giannella R. A., Washington O., Gemski P., Formal S. B. Invasion of HeLa cells by Salmonella typhimurium: a model for study of invasiveness of Salmonella. J Infect Dis. 1973 Jul;128(1):69–75. doi: 10.1093/infdis/128.1.69. [DOI] [PubMed] [Google Scholar]
  9. Gyles C. L. Limitations of the infant mouse test for Escherichia coli heat stable enterotoxin. Can J Comp Med. 1979 Oct;43(4):371–379. [PMC free article] [PubMed] [Google Scholar]
  10. Hadad J. J., Gyles C. L. Detection of bovine enteropathogenic Escherichia coli by indirect fluorescent antibody technique. Am J Vet Res. 1978 Oct;39(10):1651–1655. [PubMed] [Google Scholar]
  11. Keet E. E. Yersinia enterocolitica septicemia. Source of infection and incubation period identified. N Y State J Med. 1974 Nov;74(12):2226–2230. [PubMed] [Google Scholar]
  12. Kohl S., Jacobson J. A., Nahmias A. Yersinia enterocolitica infections in children. J Pediatr. 1976 Jul;89(1):77–79. doi: 10.1016/s0022-3476(76)80932-8. [DOI] [PubMed] [Google Scholar]
  13. Lee W. H., McGrath P. P., Carter P. H., Eide E. L. The ability of some Yersinia enterocolitica strains to invade HeLa cells. Can J Microbiol. 1977 Dec;23(12):1714–1722. doi: 10.1139/m77-247. [DOI] [PubMed] [Google Scholar]
  14. Marks M. I., Pai C. H., Lafleur L., Lackman L., Hammerberg O. Yersinia enterocolitica gastroenteritis: a prospective study of clinical, bacteriologic, and epidemiologic features. J Pediatr. 1980 Jan;96(1):26–31. doi: 10.1016/s0022-3476(80)80318-0. [DOI] [PubMed] [Google Scholar]
  15. Mäki M., Grönroos P., Vesikari T. In vitro invasiveness of Yersinia enterocolitica isolated from children with diarrhea. J Infect Dis. 1978 Nov;138(5):677–680. doi: 10.1093/infdis/138.5.677. [DOI] [PubMed] [Google Scholar]
  16. Pai C. H., Mors V. Production of enterotoxin by Yersinia enterocolitica. Infect Immun. 1978 Mar;19(3):908–911. doi: 10.1128/iai.19.3.908-911.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rabson A. R., Hallett A. F., Koornhof H. J. Generalized Yersinia enterocolitica infection. J Infect Dis. 1975 Apr;131(4):447–451. doi: 10.1093/infdis/131.4.447. [DOI] [PubMed] [Google Scholar]
  18. Sivaswamy G., Gyles C. L. The prevalence of enterotoxigenic Escherichia coli in the feces of calves with diarrhea. Can J Comp Med. 1976 Jul;40(3):241–246. [PMC free article] [PubMed] [Google Scholar]
  19. Toma S., Lior H., Quinn-Hill M., Sher N., Walker W. A. Yersinia enterocolitica infection: report of two cases. Can J Public Health. 1972 Sep-Oct;63(5):433–436. [PubMed] [Google Scholar]
  20. Toma S. Survey on the incidence of Yersinia enterocolitica in the province of Ontario. Can J Public Health. 1973 Sep-Oct;64(5):477–487. [PubMed] [Google Scholar]
  21. Winblad S., Niléhn B., Sternby N. H. Yersinia enterocolitica (Pasteurella x) in human enteric infections. Br Med J. 1966 Dec 3;2(5526):1363–1366. doi: 10.1136/bmj.2.5526.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES