Skip to main content
PMC home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1982 Jan;46(1):80–84.

Evaluation of the API 20E system for the identification of gram-negative nonfermenters from animal origin.

J A Devenish, D A Barnum
PMCID: PMC1320201  PMID: 7042055

Abstract

The API 20E system was evaluated on isolates from animals of aerobic nonfermentative and cytochrome oxidase positive Gram-negative rods. An accuracy of identification of 80% (214/268 isolates) was achieved for those organisms included in the 1976-1977 API profile index. Members of the genera Pseudomonas and Acinetobacter were identified with 100% accuracy. Organisms not included in the API profile gave either an unacceptable profile number or were incorrectly identified as Moraxella spp. When the inoculum size was increased there was better identification.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Devenish J. A., Barnum D. A. Evaluation of API 20E System and Encise Enterotube for the identification of Enterobacteriaceae of animal origin. Can J Comp Med. 1980 Jul;44(3):315–319. [PMC free article] [PubMed] [Google Scholar]
  2. Dowda H. Evaluation of two rapid methods for identification of commonly encountered nonfermenting or oxidase-positive, Gram-negative rods. J Clin Microbiol. 1977 Dec;6(6):605–609. doi: 10.1128/jcm.6.6.605-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Finklea P. J., Cole M. S., Sodeman T. M. Clinical evaluation of the minitek differential system for identification of Enterobacteriaceae. J Clin Microbiol. 1976 Nov;4(5):400–404. doi: 10.1128/jcm.4.5.400-404.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gilardi G. L. Nonfermentative gram-negative bacteria encountered in clinical specimens. Antonie Van Leeuwenhoek. 1973;39(2):229–242. doi: 10.1007/BF02578855. [DOI] [PubMed] [Google Scholar]
  5. Hofherr L., Votava H., Blazevic D. J. Comparison of three methods for identifying nonfermenting gram-negative rods. Can J Microbiol. 1978 Oct;24(10):1140–1144. doi: 10.1139/m78-186. [DOI] [PubMed] [Google Scholar]
  6. Nord C. E., Lindberg A. A., Dahlbäck A. Evaluation of five test-kits-API, AuxoTab, Enterotube, PathoTec and R-B-for identification of Enterobacteriaceae. Med Microbiol Immunol. 1974 Mar 22;159(3):211–220. doi: 10.1007/BF02121337. [DOI] [PubMed] [Google Scholar]
  7. Oberhofer T. R. Comparison of the API 20E and Oxi/Ferm systems in identification of nonfermentative and oxidase-positive fermentative bacteria. J Clin Microbiol. 1979 Feb;9(2):220–226. doi: 10.1128/jcm.9.2.220-226.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Oberhofer T. R., Rowen J. W., Cunningham G. F. Characterization and identification of gram-negative, nonfermentative bacteria. J Clin Microbiol. 1977 Feb;5(2):208–220. doi: 10.1128/jcm.5.2.208-220.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Otto L. A., Blachman U. Nonfermentative bacilli: evaluation of three systems for identification. J Clin Microbiol. 1979 Aug;10(2):147–154. doi: 10.1128/jcm.10.2.147-154.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pedersen M. M., Marso E., Pickett M. J. Nonfermentative bacilli associated with man. 3. Pathogenicity and antibiotic susceptibility. Am J Clin Pathol. 1970 Aug;54(2):178–192. doi: 10.1093/ajcp/54.2.178. [DOI] [PubMed] [Google Scholar]
  11. Pickett M. J., Pedersen M. M. Characterization of saccharolytic nonfermentative bacteria associated with man. Can J Microbiol. 1970 May;16(5):351–362. doi: 10.1139/m70-062. [DOI] [PubMed] [Google Scholar]
  12. Shayegani M., Maupin P. S., McGlynn D. M. Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria. J Clin Microbiol. 1978 Jun;7(6):539–545. doi: 10.1128/jcm.7.6.539-545.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith P. B., Tomfohrde K. M., Rhoden D. L., Balows A. API system: a multitube micromethod for identification of Enterobacteriaceae. Appl Microbiol. 1972 Sep;24(3):449–452. doi: 10.1128/am.24.3.449-452.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Swanson E. C., Collins M. T. Use of the API 20E system to identify veterinary Enterobacteriaceae. J Clin Microbiol. 1980 Jul;12(1):10–14. doi: 10.1128/jcm.12.1.10-14.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Warwood N. M., Blazevic D. J., Hofherr L. Comparison of the API 20E and Corning N/F systems for identification of nonfermentative gram-negative rods. J Clin Microbiol. 1979 Aug;10(2):175–179. doi: 10.1128/jcm.10.2.175-179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES