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. 1997 Oct 15;16(20):6281–6289. doi: 10.1093/emboj/16.20.6281

Initiation and bidirectional propagation of chromatin assembly from a target site for nucleotide excision repair.

P H Gaillard 1, J G Moggs 1, D M Roche 1, J P Quivy 1, P B Becker 1, R D Wood 1, G Almouzni 1
PMCID: PMC1326312  PMID: 9321407

Abstract

To restore full genomic integrity in a eukaryotic cell, DNA repair processes have to be coordinated with the resetting of nucleosomal organization. We have established a cell-free system using Drosophila embryo extracts to investigate the mechanism linking de novo nucleosome formation to nucleotide excision repair (NER). Closed-circular DNA containing a uniquely placed cisplatin-DNA adduct was used to follow chromatin assembly specifically from a site of NER. Nucleosome formation was initiated from a target site for NER. The assembly of nucleosomes propagated bidirectionally, creating a regular nucleosomal array extending beyond the initiation site. Furthermore, this chromatin assembly was still effective when the repair synthesis step in the NER process was inhibited.

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